Bioprospecting PGPR Microflora by Novel Immunobased Techniques

Agrawal, Ruchi; Verma, Amit; Satlewal, Alok

doi:10.1016/b978-0-444-63987-5.00024-4

Cited by 5 publications

(5 citation statements)

References 55 publications

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“…Hydrolysate samples were taken periodically and analyzed for cellobiose and total phenols using Waters' HPLC with Aminex HPX-87P column (Bio-Rad, United States) coupled with refractive index detector (Agrawal et al, 2018). Milli-Q water was used as a mobile phase at a flow rate of 0.6 ml/min, with a column temperature of 75 • C (Agrawal et al, 2015b).…”

Section: Cellobiose and Total Phenols Estimationsmentioning

confidence: 99%

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

et al. 2018

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Enzymatic hydrolysis at high solids loading has the potential to reduce both capital and operational expenditures. Here, pretreatment of rice straw (PRS) with dilute acid was carried out at a pilot scale (250 kg per day) at 162 • C for 10 min and 0.35% acid concentration, followed by enzymatic hydrolysis at different total solids loadings. It showed that although the total sugar concentration increased from 48 to 132 g/l, glucan conversion reduced by 27% (84-66.2%) with increasing solids from 5 to 20% in batch mode. Therefore, two different fed-batch approaches were evaluated to improve the glucan conversion by the sequential addition of a substrate and/or enzyme. At 20% solid loadings and a 3 filter paper units/g enzyme dosage, the highest glucan conversion obtained was 66% after 30 h of hydrolysis in batch mode. However, in an optimized fed-batch approach, the glucan yield was improved to 70% by simply dividing the substrate feeding into three batches, that is, 50% at 0 h, 25% each after 4 h, and 8 h of hydrolysis reaction. The addition of surfactant (Ecosurf E6) further improved the conversion to 72% after 30 h. The role of critical factors, that is, inhibitors, enzyme-lignin binding, and viscosity, was investigated during the course of hydrolysis in the batch and fed-batch approaches. This study suggests a sustainable approach for improved hydrolysis at high solids loadings by fine-tuning a simple process.

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Section: Cellobiose and Total Phenols Estimationsmentioning

confidence: 99%

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

et al. 2018

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“…The protein content of the Accellerase 1500 enzyme was determined as 82 mg/mL by a Bicinchoninic Acid (BCA) protein kit. , Protein loading of Accellerase 1500 was 10 and 50 mg/g glucan in the untreated biomass. Aliquots of an equal amount of 0.2 mL were withdrawn after shaking at 4, 24, 48, 72, and 120 h and quenched for 10 min in a boiling water bath followed by centrifugation (MiniSpin Plus, Eppendorf AG, Hauppauge, NY) at 10 000 rpm for 5 min. , The liquid supernatants were then frozen to −20 °C until sugar quantification was accomplished. The supernatants were diluted and injected to high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using a Dionex ICS-3000 (Dionex Corp. in Sunnyvale, CA) equipped with an electrochemical detector, a CarboPac PA1 guard column (2 × 50 mm, Dionex), a CarboPac PA1 column (2 × 250 mm, Dionex), an AS40 automated sampler, and a PC 10 pneumatic controller at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Assessing the Facile Pretreatments of Bagasse for Efficient Enzymatic Conversion and Their Impacts on Structural and Chemical Properties

Satlewal

Agrawal

Das

et al. 2018

ACS Sustainable Chem. Eng.

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Novel and sustainable pretreatment approaches are desired to improve the techno-commercial feasibility of biorefineries in the future. In this study, 10 renewable deep eutectic solvents (DESs) were evaluated for their pretreatment efficiency at facile conditions with sugar cane bagasse as substrate and compared with conventional pretreatment approaches (dilute alkali, dilute acid, and ionic liquid (IL)) for lignin removal, saccharification yield, cellulose accessibility, crystallinity, and physiochemical properties. Although, the highest delignification was obtained with dilute alkali (59.7%) and choline chloride:lactic acid or ChCl:LA (50.6%), the maximum enzymatic conversion of 98.0% and 90.4% was observed with IL (1-butyl-3-methylimidazolium acetate) and ChCl:LA, respectively. uclear magnetic resonance analysis of ChCl:LA-derived lignin showed selective removal of guaiacyl lignin without condensation structure formation observed. Interestingly, unlike IL, the lignin was substantially depolymerized after ChCl:LA pretreatment as determined by gel permeation chromatography. Further, high compatibility of ChCl:LA with cellulase in comparison of IL with easy recyclability and recycling showed that DESs synthesized from a renewable resource are promising “green” solvents for future biorefinery operations.

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“…The traits mentioned above determine the functional characterisation of the PGPR, which include phytostimulation, phytoremediation, bio-fertilisation and biocontrol agents (BCAs) [58]. Successful colonisation of the rhizosphere due to nutrient availability supports bacterial accumulation, growth and metabolism [59]. Among the rhizobacterial communities, the most common and widely explored genera include Pseudomonas, Bacillus, Xanthomonas, Rhizobium, Arthrobacter, Azospirillum, Azobacter and Agrobacterium [59].…”

Section: Rhizosphere Plant-pgpr Communications and Pgpr Mechanisms Of...mentioning

confidence: 99%

“…Successful colonisation of the rhizosphere due to nutrient availability supports bacterial accumulation, growth and metabolism [59]. Among the rhizobacterial communities, the most common and widely explored genera include Pseudomonas, Bacillus, Xanthomonas, Rhizobium, Arthrobacter, Azospirillum, Azobacter and Agrobacterium [59]. Over the years, Pseudomonas and Bacillus species have been widely investigated for plant growth promotion, phytopathogen antagonism and plant priming with successful root colonisation [57].…”

Section: Rhizosphere Plant-pgpr Communications and Pgpr Mechanisms Of...mentioning

confidence: 99%

Rhizosphere Tripartite Interactions and PGPR-Mediated Metabolic Reprogramming towards ISR and Plant Priming: A Metabolomics Review

Mashabela

Piater

Dubery

et al. 2022

Biology

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Plant growth-promoting rhizobacteria (PGPR) are beneficial microorganisms colonising the rhizosphere. PGPR are involved in plant growth promotion and plant priming against biotic and abiotic stresses. Plant–microbe interactions occur through chemical communications in the rhizosphere and a tripartite interaction mechanism between plants, pathogenic microbes and plant-beneficial microbes has been defined. However, comprehensive information on the rhizosphere communications between plants and microbes, the tripartite interactions and the biochemical implications of these interactions on the plant metabolome is minimal and not yet widely available nor well understood. Furthermore, the mechanistic nature of PGPR effects on induced systemic resistance (ISR) and priming in plants at the molecular and metabolic levels is yet to be fully elucidated. As such, research investigating chemical communication in the rhizosphere is currently underway. Over the past decades, metabolomics approaches have been extensively used in describing the detailed metabolome of organisms and have allowed the understanding of metabolic reprogramming in plants due to tripartite interactions. Here, we review communication systems between plants and microorganisms in the rhizosphere that lead to plant growth stimulation and priming/induced resistance and the applications of metabolomics in understanding these complex tripartite interactions.

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Bioprospecting PGPR Microflora by Novel Immunobased Techniques

Cited by 5 publications

References 55 publications

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

Assessing the Facile Pretreatments of Bagasse for Efficient Enzymatic Conversion and Their Impacts on Structural and Chemical Properties

Rhizosphere Tripartite Interactions and PGPR-Mediated Metabolic Reprogramming towards ISR and Plant Priming: A Metabolomics Review

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