Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

Agrawal, Ruchi; Bhadana, Bharti; Mathur, Anshu S.; Kumar, Ravindra; Gupta, Ravi P.; Satlewal, Alok

doi:10.3389/fenrg.2018.00115

Cited by 38 publications

(20 citation statements)

References 51 publications

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“…through a reduction in the enzyme dosage required for achieving similar cellulose conversion on pretreated spruce, where 6 g/100 g red saponin dosage combined with 7.5 FPU/g cellulase loading gave a conversion comparable to that achieved by using 4 g/100 g saponins and 10 FPU/g. (A) (B) The concentrations of glucose obtained in this study are higher than those achieved by Nogueira et al [42] and Li [43] using PEG4000 as surfactant; however, the values are lower compared with the studies reported by Vignesh et al [42] and Agrawal et al [44]. Some of the works reported in Table 6 used high solid pretreated loading in the enzymatic hydrolysis process (20-35%, w/v); therefore, the operation of high solid loading is an important operative strategy that together with the use of surfactants can overcome the development of second-generation biorefineries producing high concentrations of fermentable sugars.…”

Section: Enzyme Loading Evaluationcontrasting

confidence: 69%

Hot Compressed Water Pretreatment and Surfactant Effect on Enzymatic Hydrolysis Using Agave Bagasse

et al. 2021

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Agave bagasse is a residual biomass in the production of the alcoholic beverage tequila, and therefore, it is a promising raw material in the development of biorefineries using hot compressed water pretreatment (hydrothermal processing). Surfactants application has been frequently reported as an alternative to enhance monomeric sugars production efficiency and as a possibility to reduce the enzyme loading required. Nevertheless, the surfactant’s action mechanisms in the enzymatic hydrolysis is still not elucidated. In this work, hot compressed water pretreatment was applied on agave bagasse for biomass fractionation at 194 °C in isothermal regime for 30 min, and the effect of non-ionic surfactants (Tween 20, Tween 80, Span 80, and Polyethylene glycol (PEG 400)) was studied as a potential enhancer of enzymatic saccharification of hydrothermally pretreated solids of agave bagasse (AGB). It was found that non-ionic surfactants show an improvement in the conversion yield of cellulose to glucose (100%) and production of glucose (79.76 g/L) at 15 FPU/g glucan, the highest enhancement obtained being 7% regarding the control (no surfactant addition), using PEG 400 as an additive. The use of surfactants allows improving the production of fermentable sugars for the development of second-generation biorefineries.

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Section: Enzyme Loading Evaluationcontrasting

confidence: 69%

Hot Compressed Water Pretreatment and Surfactant Effect on Enzymatic Hydrolysis Using Agave Bagasse

et al. 2021

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“…The VS content is an indicator of the biodegradability of the samples and represents the portion of substrate that can be converted into biogas and biomethane. Research has shown that high VS content is a desirable condition in the anaerobic digestion process since it leads to higher biogas and biomethane yields [51,52].…”

Section: Chemical Compositionmentioning

confidence: 99%

Bioenergy Yields from Sequential Bioethanol and Biomethane Production: An Optimized Process Flow

et al. 2019

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This study investigates the potential of different stages of the bioethanol production process (pretreatment, hydrolysis, and distillation) for bioethanol and biomethane production, and studies the critical steps for the liquid and the solid fractions to be separated and discarded to improve the efficiency of the production chain. For this, Napier grass (a fast-growing grass) from Effurun town of Delta State in Nigeria was used and the novel pretreatment method, nitrogen explosive decompression (NED), was applied at different temperatures. The results show that the lowest glucose (13.7 g/L) and ethanol titers (8.4 g/L) were gained at 150 • C. The highest glucose recovery (31.3 g/L) was obtained at 200 • C and the maximum ethanol production (10.3 g/L) at 170 • C. Methane yields are higher in samples pretreated at lower temperatures. The maximum methane yields were reported in samples from the solid fraction of post-pretreatment (pretreated at 150 • C, 1.13 mol CH4/100 g) and solid fraction of the post-hydrolysis stage (pretreated at 150 • C, 1.00 mol CH4/100 g). The lowest biomethane production was noted in samples from the liquid fraction of post-pretreatment broth (between 0.14 mol CH4/100 g and 0.24 mol CH4/100 g). From the process point of view, samples from liquid fraction of post-pretreatment broth should be separated and discarded from the bioethanol production process, since they do not add value to the production chain. The results suggest that bioethanol and biomethane concentrations are influenced by the pretreatment temperature. Napier grass has potential for bioethanol and further biomethane production and it can be used as an alternative source of energy for the transportation sector in Nigeria and other countries rich in grasses and provide energy security to their population.alternative sources of energy that will decrease the share of fossil fuels utilised in the transportation sector and productive uses, reduce environmental concerns caused by the extensive utilization of these resources (e.g., climate change and global warming), increase energy security, and improve the access of the population to electricity. Renewable energy sources from lignocellulosic biomass have been reported as promising solutions to these problems [4]. Nowadays, biomass itself is responsible for 10% primary energy consumption worldwide [5] and it can be used as a promising feedstock for biofuel production. The sustainability of wastes and by-products as a biofuel for the transportation sector in a circular economy has been studied in the literature, in order to make the biogas-biomethane chain more sustainable [6][7][8]. These studies show the positive impact of biofuels in the transportation section in Europe. However, further research needs to be done in order to apply circular economy models to emerging economies.Sub-Saharan Africa has great potential to develop renewable energy sources, such as wind, biomass, solar, and hydro. Just in Nigeria, the biomass potential is about 144 million tonnes per year and the potenti...

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“…Concerning the TS composition, in both fractions (solid and liquid), the samples taken from the hydrolysis step had the highest levels of TS content, probably due to the added enzyme which works as a catalyst, improving hydrolysis efficiency and leading to higher TS contents [20]. In almost all situations, samples from the pretreatment stage had the lowest TS values.…”

Section: Chemical Compositionmentioning

confidence: 99%

Enhancing Bioenergy Yields from Sequential Bioethanol and Biomethane Production by Means of Solid–Liquid Separation of the Substrates

et al. 2019

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The production of second-generation ethanol using lignocellulosic feedstock is crucial in order to be able to meet the increasing fuel demands by the transportation sector. However, the technology still needs to overcome several bottlenecks before feasible commercialization can be realized. These include, for example, the development of cost-effective and environmentally friendly pretreatment strategies and valorization of the sidestream that is obtained following ethanol distillation. This work uses two chemical-free pretreatment methods—nitrogen explosive decompression (NED) and synthetic flue gas explosive decompression—to investigate the potential of a bioethanol production sidestream in terms of further anaerobic digestion. For this purpose, samples from different stages of the bioethanol production process (pretreatment, hydrolysis, and fermentation) and the bioethanol sidestream went through a separation process (involving solid–liquid separation), following which a biomethane potential (BMP) assay was carried out. The results show that both factors being studied in this article (involving the pretreatment method and the separation process) served to influence methane yields. Liquid fractions that were obtained during the process with NED gave rise to methane yields that were 8% to 12% higher than when synthetic flue gas was used; fermented and distillation sidestream gave rise to the highest methane yields (0.53 and 0.58 mol CH4/100 g respectively). The methane yields from the liquid fractions were between 60–88% lower than those that were obtained from solid fractions. Samples from the bioethanol sidestream (solid fraction) that were pretreated with NED had the highest methane yield (1.7 mol CH4/100 g). A solid–liquid separation step can be a promising strategy when it comes to improving the energy output from lignocellulosic biomass and the management of the ethanol distillation sidestream.

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Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

Cited by 38 publications

References 51 publications

Hot Compressed Water Pretreatment and Surfactant Effect on Enzymatic Hydrolysis Using Agave Bagasse

Hot Compressed Water Pretreatment and Surfactant Effect on Enzymatic Hydrolysis Using Agave Bagasse

Bioenergy Yields from Sequential Bioethanol and Biomethane Production: An Optimized Process Flow

Enhancing Bioenergy Yields from Sequential Bioethanol and Biomethane Production by Means of Solid–Liquid Separation of the Substrates

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