Bioprospecting highly diverse endophytic Pestalotiopsis spp. with antibacterial properties from Maytenus ilicifolia, a medicinal plant from Brazil

Figueiredo, Josiane Aparecida Gomes; Pimentel, Ida Chapaval; Vicente, Vânia A.; Pie, Márcio R.; Kava, Vanessa; Galli-Terasawa, Lygia Galli-TerasawaL.; Pereira, José Odair; Souza, Antonia de SouzaA.Q.L. de Q.L.; Glienke, Chirlei

doi:10.1139/w07-078

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…Other studies analyzing the interactions between endophytic isolates and different pathogens demonstrated a reduction in pathogens growth ranging from 28.5 to 78.8% (Gomes-Figueiredo et al, 2007;Campanile et al, 2007;Sempere andSantamarina, 2007, Bailey et al, 2008). Crozier et al (2006), also studying the phytopathogens Moniliophthora roreri and M. perniciosa, the causative agents of fresh green bean rot and witches' broom disease, respectively, used endophytic fungi to combat pathogens and showed that the endophytic fungi of this plant could prevent the initial colonization of the pathogens competing for the same ecological niche and invading them.…”

Section: Discussionmentioning

confidence: 98%

Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria

Bernardi-Wenzel¹,

García²,

Azevedo³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Luehea divaricata is an important plant in popular medicine; it is used for its depurative, anti-inflammatory, and other therapeutic activities. We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this evaluation of antimicrobial activity, we extracted secondary metabolites from nine endophytic fungi by partitioning in ethyl acetate and methanol. These were tested against the phytopathogens A. alternata, Colletotrichum sp and Moniliophthora perniciosa, and against the human pathogenic bacteria Escherichia coli and Staphylococcus aureus. Molecular characterization by ARDRA technique was used for phylogenetic analysis, based on comparison with sequences in GenBank. The endophytes had varied effects on A. alternata. One isolate produced an inhibition halo against M. perniciosa and against E. coli. This antibiosis activity indicates a role in the protection of the plant against microbial pathogens in nature, with potential for pharmaceutical and agricultural applications. Based on ARDRA, the 13 isolates were grouped. We found three different haplotypes of Phomopsis sp, showing interspecific variability. It appears that examination of the microbial community associated with medicinal plants of tropical regions has potential as a useful strategy to look for species with biotechnological applications.

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Section: Discussionmentioning

confidence: 98%

Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria

Bernardi-Wenzel¹,

García²,

Azevedo³

et al. 2013

Genet. Mol. Res.

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“…Endophytic fungi inhabiting plants with a well-established ethnobotanical history represent a unique ecological group, since the medicinal effects of the plant could also be related to the resident endophytes (Kusari, Pandey, et al 2013). In the last 10 years, there has been an upsurge of studies that have targeted fungal endophytes from medicinal plants for the isolation and characterization of novel metabolites (Wang et al 2002; Tejesvi et al 2006; Gomes-Figueiredo et al 2007; Huang et al 2008; Hyde and Soytong 2008; Naik et al 2008; Krishnamurthy et al 2009; Bills et al 2012; Gond et al 2012; Mishra et al 2012; Vieira et al 2012; Zubek et al 2012; Miller, Qing, Sze, Neilan, et al 2012; Aly et al 2013; Chen et al 2013; Ellsworth et al 2013; Kusari, Kusari, et al 2013). In the present study, we have isolated four new secondary metabolites [biscognin A ( 1 ), biscognin B ( 3 ), monascuskaoliaone B ( 4 ), and epi -pestalamide A ( 6 )] from milk thistle endophytes, in addition to scores of known compounds that are members of a range of different classes of natural products.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and chemical diversity of fungal endophytes isolated fromSilybum marianum(L) Gaertn. (milk thistle)

et al. 2015

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Use of the herb milk thistle (Silybum marianum) is widespread, and its chemistry has been studied for over 50 years. However, milk thistle endophytes have not been studied previously for their fungal and chemical diversity. We examined the fungal endophytes inhabiting this medicinal herb to determine: (1) species composition and phylogenetic diversity of fungal endophytes; (2) chemical diversity of secondary metabolites produced by these organisms; and (3) cytotoxicity of the pure compounds against the human prostate carcinoma (PC-3) cell line. Forty-one fungal isolates were identified from milk thistle comprising 25 operational taxonomic units based on BLAST search via GenBank using published authentic sequences from nuclear ribosomal internal transcribed spacer sequence data. Maximum likelihood analyses of partial 28S rRNA gene showed that these endophytes had phylogenetic affinities to four major classes of Ascomycota, the Dothideomycetes, Sordariomycetes, Eurotiomycetes, and Leotiomycetes. Chemical studies of solid–substrate fermentation cultures led to the isolation of four new natural products. In addition, 58 known secondary metabolites, representing diverse biosynthetic classes, were isolated and characterized using a suite of nuclear magnetic resonance and mass spectrometry techniques. Selected pure compounds were tested against the PC-3 cell line, where six compounds displayed cytotoxicity.

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“…They observed strong in vitro cytotoxicity for KB cancer cells (human epidermal carcinoma), as well as for P388 cells (murine leukemia). In a study in Brazil, Gomes-Figueiredo et al (2007) characterized endophytic Pestalotiopsis from Maytenus ilicifolia (espinheira-santa), which showed antimicrobial activity against various human pathogens.…”

Section: Introductionmentioning

confidence: 99%

Molecular and morphological markers for rapid distinction between 2 Colletotrichum species

et al. 2009

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Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, however, has been a problem in this type of study. Several authors have reported the presence of endophytic fungi from the medicinal plant Maytenus ilicifolia ("espinheira-santa") in Brazil that has antimicrobial activity against various pathogens. Therefore, Colletotrichum strains were isolated from M. ilicifolia and identified based on morphology, RAPD markers, sequence data of the internal transcribed spacer regions (ITS-1 and ITS-2), the 5.8S gene, and species-specific PCR. The analyses suggested the presence of 2 species, Colletotrichum gloeosporioides and Colletotrichum boninense. Two morphological markers were characterized to allow C. gloeosporioides and C. boninense to be distinguished quickly and accurately. The molecular diagnosis of C. boninense was confirmed by using Col1 and ITS4 primers. This species of Colletotrichum is reported for the first time in M. ilicifolia.

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Bioprospecting highly diverse endophytic Pestalotiopsis spp. with antibacterial properties from Maytenus ilicifolia, a medicinal plant from Brazil

Cited by 16 publications

References 28 publications

Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria

Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria

Phylogenetic and chemical diversity of fungal endophytes isolated fromSilybum marianum(L) Gaertn. (milk thistle)

Molecular and morphological markers for rapid distinction between 2 Colletotrichum species

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