Bioprospecting and biotechnological applications of fungal laccase

Abstract

Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin. Laccases can degrade both phenolic and non-phenolic compounds. They also have the ability to detoxify a range of environmental pollutants. Due to their property to detoxify a range of pollutants, they have been used for several purposes in many industries including paper, pulp, textile and petrochemical industries. Some other application of laccase includes in food processing industry, medical and health care. Recently, laccase has found applications in other fields such as in the design of biosensors and nanotechnology. The present review provides an overview of biological functions of laccase, its mechanism of action, laccase mediator system, and various biotechnological applications of laccase obtained from endophytic fungi.

Enhanced delignification of lignocellulosic substrates by <i>Pichia</i> GS115 expressed recombinant laccase

“…There is a continuous quest for isolating and screening laccase producers with desirable yields and properties [Olajuyigbe and Fatokun, 2017]. Hatomoto et al [1999] expressed S. commune laccase in Aspergillus sajae strain 1860 (with low protease levels) suggesting protein engineering strategies for improved production [Upadhyay et al, 2016]. In the present study, the laccase gene was codon optimized as per the codon bias of P.pastoris [Wang et al, 2012;Mellitzer et al, 2012].…”

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

“…These enzymes are generally extracellular and catalyze the oxidation of several aromatic amines, phenolic compounds, thiols and some inorganic compounds using molecular oxygen as electron acceptor (Madhavi and Lele 2009). With these properties, they have attracted the attention of many researchers; they have potential applications and used in many fields, including the environmental and industrial sectors such as pharmaceutical, nano-biotech, textile, paper and pulp, food, chemistry, cosmetic due to their low substrate specificities and high redox potentials (Madhavi and Lele 2009;Upadhyay et al 2016). Moreover, several dyes are commonly use as a colorant in these textile, food, pharmaceutical, paper printing, and photographic, cosmetic, and leather industries (Rodriguez-Couto 2009).…”

Evaluation of Catalytic Efficiency of Coriolopsis caperata DN Laccase to Decolorize and Detoxify RBBR Dye

“…Hence, laccase and laccase-mediated system (LMS)-assisted dye decolorization may provide a reasonable basis for the development of biotechnological processes for removal of color and aromatic compounds from various industrial effluents at large scale [8][9][10]. For development of sustainable technology, it is necessary to isolate new microorganisms and enzymes, with broader substrate specificity and higher activity to control pollution [6].…”