Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections

Hage, Salim; Morlock, Gertrud E.

doi:10.1016/j.chroma.2017.02.019

Cited by 49 publications

(24 citation statements)

References 62 publications

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“…Moreover, the biomass of different willow species may be a source of other bioactive compounds, especially lignans, flavonoids and tannins. They have various biological activities, especially antioxidant, anticancer and antibacterial (Brereton et al 2017;Dimkić et al 2016;Hage and Morlock 2017;Koirala et al 2016). The species, clones and varieties cultivated in short rotation coppice are characterized by a great potential in this respect.…”

Section: Willowmentioning

confidence: 99%

“…The smallest amount of the compound was observed in S. purpurea extract (Pobłocka-Olech and Krauze-Baranowska 2008). The HPTLC method was also used to separate bioactive compounds from S. alba buds, but also to test their antibacterial and biochemical activity (Hage and Morlock 2017). Phenolic acids, flavonoids as well as non-salicylic glucosides were analyzed in methanolic extracts of willow (S. myrsinifolia) leaves and bark with the use of HPLC-DAD method.…”

Section: Willow Non-salicylic Bioactive Compoundsmentioning

confidence: 99%

“…It was also found that the poplar resins contained much higher concentrations of flavonoids and phenolic acids than S. alba resins. Hage and Morlock (2017) used HPTLC method for bioprofiling of poplar bud extracts. TLC plate silica gel with separated phenolic compounds was subjected to antimicrobial, estrogen-like activity and esterase assays.…”

Section: Poplarmentioning

confidence: 99%

“…They are important substances as ingredients of food, herbal raw materials as well as medicines and dietary supplements produced from vegetable raw materials. In general, the antioxidant as well as antibacterial and antifungal properties are assigned to natural antioxidants (Aguirre and Borneo 2013;Dimkić et al 2016;Hage and Morlock 2017;Marinas et al 2014). The spectrophotometric and fluorimetric methods of TAC determination are used to assess the biological activity of raw materials and plant products.…”

Section: Total Antioxidant Capacity (Tac) Total Phenolic Content (Tpmentioning

confidence: 99%

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Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Tyśkiewicz

Konkol

Kowalski

et al. 2019

Trees

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Key message Liquid chromatography is the main method for assaying bioactive compounds in biomass. Total phenolic content and antioxidant capacity are measured with spectroscopy. Phenolics are the most important bioactive substances in short rotation coppice. Secondary metabolites of black locust, willow and poplar have many different activities. The richest source of bioactive compounds are flowers, bark and buds. Abstract The black locust (Robinia pseudoacacia), willow (Salix spp.) and poplar (Populus spp.) may be the source of the biomass obtained from natural habitats as well as intentionally established plantations in the system of short rotation coppice. These species are promising candidates as an alternative source of bioactive compounds in researches for developing new bioproducts for multidirectional uses, including polymers, lubricants, construction materials, pharmaceuticals, as well as bioenergy and fuels. Various parts of plants, such as leaves, flowers, seeds, bark, woods, buds, catkins, whole tree material and bee products (honey, propolis) are a source of bioactive compounds. The characterization of these compounds, especially phenolic compounds (flavonoids, stilbenes, lignans, phenolic acids, tannins and phenolic glucosides), fatty acids, sterols, etc. may be determined by a variety of analytical (spectroscopy, chromatography) and extraction methods. This review contains 131 references and systematize all available data on the characterization of the bioactive compounds in the biomass of black locust, poplar and willow by analytical and extraction methods.

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Section: Willowmentioning

confidence: 99%

Section: Willow Non-salicylic Bioactive Compoundsmentioning

confidence: 99%

Section: Poplarmentioning

confidence: 99%

Section: Total Antioxidant Capacity (Tac) Total Phenolic Content (Tpmentioning

confidence: 99%

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Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Tyśkiewicz

Konkol

Kowalski

et al. 2019

Trees

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“…For example, Cai et al reported that 1,4‐dihydropyridines could form [M − H] + ion by an electrochemical oxidation during the electrospray process. The second type is the compounds with rich π‐electron cloud . For this type of compounds, chiral C‐3 alkyl‐substituted indoles, diarylmethylamine, cyclopenta[b] indoles, and bisindolylmethanes were designed for the formation of [M − H] + ion in gas phase.…”

Section: Introductionmentioning

confidence: 99%

Hydralazine derivative of aldehyde: A new type of [M − H]⁺ ion formed in electrospray ionization mass spectrometry

et al. 2019

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Hydralazine has been widely employed in the development of drugs, derivatization reagents, and ligands. In the present work, we reported a new type of dehydrogenated ion [M − H] + that was produced from the hydralazine derivative of hexanal in electrospray ionization mass spectrometry (ESI-MS). The formation of [M − H] + ions in the ESI-MS was found to be independent on the mobile phase composition of the liquid chromatography and ESI source parameters. A series of hydralazine derivatives of aldehyde were investigated to confirm this phenomenon. The results showed that hydralazine derivatives of aldehydes that contained an sp 3 hybridization carbon with a hydrogen at the α-position of aldehydes could form the unexpected [M − H] + ions, whereas hydralazine derivative of acetone could only generate [M + H] + ion in the ESI-MS. We proposed the possible formation mechanism of [M − H] + ion for the hydralazine derivatives of aldehydes: the [M − H] + ion was possibly formed by the loss a hydrogen molecule (H 2 ) from the protonated ion [M + H] + . The results obtained from density functional theory (DFT) calculations supported this proposed formation mechanism of [M − H] + ion.

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Bioautography‐guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species

Anokwuru

Chen

Vuuren

et al. 2022

Phytochemical Analysis

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Introduction: Many species within Combretaceae are traditionally used for the treatment of bacterial infections. The similarity in chemistry and antimicrobial activities within the family pose a challenge in selecting suitable species for herbal drug development.Objective: This study aimed at rapidly identifying antimicrobial compounds using bioautography-guided high-performance thin-layer chromatography coupled with mass spectrometry (HPTLC-MS).Methods: Hierarchical cluster analysis of ultra-performance liquid chromatographymass spectrometry data from the methanol extracts of 77 samples, representing four genera within Combretaceae, was carried out. Based on groupings on the dendrogram, 15 samples were selected for bioautography analysis against four pathogens (Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhimurium).Active compounds were identified using HPTLC-MS analysis of bands corresponding to the inhibition zones.Results: Bioautography revealed 15 inhibition zones against the four pathogens, with the most prominent present for Combretum imberbe. Analysis of the active bands, using HPTLC-MS indicated that flavonoids, triterpenoids and combretastatin B5 contributed to the antibacterial activity. The compounds corresponding to molecular ions m/z 471 (Combretum imberbe) and 499 (Combretum elaeagnoides) inhibited all four pathogens, and were identified as imberbic acid and jessic acid, respectively. Chemotaxonomic analysis indicated that arjunic acid, ursolic acid and an unidentified triterpenoid (m/z 471) were ubiquitous in the Combretaceae species and could be responsible for their antibacterial activities. Conclusion:Application of HPTLC-MS enabled the rapid screening of extracts to identify active compounds within taxonomically related species. This approach allows for greater efficiency in the natural product research workflow to identify bioactive compounds in crude extracts.

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Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections

Cited by 49 publications

References 62 publications

Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Hydralazine derivative of aldehyde: A new type of [M − H]⁺ ion formed in electrospray ionization mass spectrometry

Bioautography‐guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species

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Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections

Cited by 49 publications

References 62 publications

Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Characterization of bioactive compounds in the biomass of black locust, poplar and willow

Hydralazine derivative of aldehyde: A new type of [M − H]+ ion formed in electrospray ionization mass spectrometry

Bioautography‐guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species

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Hydralazine derivative of aldehyde: A new type of [M − H]⁺ ion formed in electrospray ionization mass spectrometry