Bioautography‐guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species

Anokwuru, Chinedu P.; Chen, Weiyang; Vuuren, Sandy van; Combrinck, Sandra; Viljoen, Alvaro

doi:10.1002/pca.3167

Cited by 6 publications

(4 citation statements)

References 43 publications

(95 reference statements)

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“…They are first isolated from the South African Combretum caffrum specie [59]. Other Combretum species also contain these molecules [60,61]. Combretastatins are simple compounds formed of two phenyl rings linked by a chain of two carbon atoms.…”

Section: Potential Anticancer Compounds Isolated From Combretum Speciesmentioning

confidence: 99%

Traditional Uses and Anticancer Potential of the Combretum Genus: A Literature Review

Coulidiati

2023

EJMP

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Background: Cancer incidence is increasing annually in all countries. So, it is nowadays a great burden for the different nations of the world. Research for new therapeutics is becoming an urgent need, particularly for intractable and chemoresistant cancer cases. The solutions can still be found by investigating natural products which are recognized as promising sources of bioactive compounds with a potential for the discovery of new preventive and therapeutic anticancer agents. Methodology: The present work used databases such as Pubmed, Science Direct and Google scholar to investigate the ethnobotanical uses of some Combretum species in the literature. It also allowed us to summarize some pharmacological studies on Combretum species. Results: This review gathers all available traditional uses and cytotoxicity studies of Combretum species in the literature. Special focus is given to pharmacological studies highlighting isolated potential anticancer molecules. These molecules present potent cytotoxic effect on various cancer cell lines and may contribute to improving the health of people suffering from various cancer diseases. Conclusion: The Combretum species are widely used in folk medicine for the treatment of several pathologies including cancers. This study is of fundamental importance in highlighting Combretum species as a potential source for research of new anticancer compounds.

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Section: Potential Anticancer Compounds Isolated From Combretum Speciesmentioning

confidence: 99%

Traditional Uses and Anticancer Potential of the Combretum Genus: A Literature Review

Coulidiati

2023

EJMP

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“…Therefore, it is essential to develop a rapid and effective analytical method for identifying the chemical components of GS systematically and comprehensively. Ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight–tandem mass spectrometry (UHPLC–QTOF–MS/MS) coupled with data mining strategy allows rapid identification of Chinese herbal medicine constituents and detection of potential new compounds 13–18 ; due to these advantages over traditional extraction and separation methods, the technology is widely utilized in the manifestation of TCM ingredients 19–24 …”

Section: Introductionmentioning

confidence: 99%

“…Ultra-high-performance liquid chromatography-quadrupole time-offlight-tandem mass spectrometry (UHPLC-QTOF-MS/MS) coupled with data mining strategy allows rapid identification of Chinese herbal medicine constituents and detection of potential new compounds [13][14][15][16][17][18] ; due to these advantages over traditional extraction and separation methods, the technology is widely utilized in the manifestation of TCM ingredients. [19][20][21][22][23][24] At present, only a few comprehensive profiles of GS chemical composition have been reported.…”

mentioning

confidence: 99%

Comprehensive profiling of chemical composition of Gleditsiae spina using ultra‐high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry

Bai

Jing

Yang

et al. 2023

Rapid Comm Mass Spectrometry

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RationaleGleditsiae spina (GS) is an important herb used in traditional and folk medicinal systems of East Asian countries for its various medicinal properties. In China, it has been traditionally used through the centuries for its anticancer, detoxication, detumescence, apocenosis, and antiparasitic effects. Although some of its ingredients have been isolated and identified, most active constituents remain unknown. Past research mostly exploited nuclear magnetic resonance for the identification of compounds, which is suitable for monomers only. Moreover, the extraction and isolation procedures for obtaining purified molecules are time consuming. Therefore, establishing an efficient approach will assist in rapid discovery of the potential active ingredients of GS. The present study aimed to identify the chemical constituents in GS by a data analysis strategy using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight tandem mass spectrometry.MethodsFirst, the theoretical formula of the candidate compound was calculated using the accurate mass of the precursor/adduct ions. Second, the compounds were classified by the diagnostic ions from the MS/MS data. Third, characteristic ion filtering was used to identify the structures. Finally, the diverse skeletons and substitutions were further identified through the neutral loss in the GS.ResultsA total of 277 compounds were identified in GS, comprising 169 flavonoids, 70 lignans, and 38 other compounds. At least 43 potential new compounds were represented.ConclusionsThis experiment devised an efficient and systematic method for detecting complex compounds and provided a foundation for future research into bioactive ingredients and quality control of GS.

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“…These compounds are common for the Combretaceae family in general and for L. racemosa in particular. Recently, triterpene acids were identified as anti-bacterial compounds in Combretaceae [28], and their effect against Staphyllococcus aureus was proposed to be a result of synergism with epicatechin [28]. However, not all bioactive compounds of mangroves originate from the plant itself, with many produced by associated microorganisms [6,[29][30][31], including various fungal endophytes.…”

Section: Introductionmentioning

confidence: 99%

Challenging Structure Elucidation of Lumnitzeralactone, an Ellagic Acid Derivative from the Mangrove Lumnitzera racemosa

et al. 2023

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The previously undescribed natural product lumnitzeralactone (1), which represents a derivative of ellagic acid, was isolated from the anti-bacterial extract of the Indonesian mangrove species Lumnitzera racemosa Willd. The structure of lumnitzeralactone (1), a proton-deficient and highly challenging condensed aromatic ring system, was unambiguously elucidated by extensive spectroscopic analyses involving high-resolution mass spectrometry (HRMS), 1D 1H and 13C nuclear magnetic resonance spectroscopy (NMR), and 2D NMR (including 1,1-ADEQUATE and 1,n-ADEQUATE). Determination of the structure was supported by computer-assisted structure elucidation (CASE system applying ACD-SE), density functional theory (DFT) calculations, and a two-step chemical synthesis. Possible biosynthetic pathways involving mangrove-associated fungi have been suggested.

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Bioautography‐guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species

Cited by 6 publications

References 43 publications

Traditional Uses and Anticancer Potential of the Combretum Genus: A Literature Review

Traditional Uses and Anticancer Potential of the Combretum Genus: A Literature Review

Comprehensive profiling of chemical composition of Gleditsiae spina using ultra‐high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry

Challenging Structure Elucidation of Lumnitzeralactone, an Ellagic Acid Derivative from the Mangrove Lumnitzera racemosa

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