Bioprinting and regeneration of auricular cartilage using a bioactive bioink based on microporous photocrosslinkable acellular cartilage matrix

Jia, Litao; Hua, Yujie; Zeng, Jinshi; Liu, Wenshuai; Wang, Di; Zhou, Guangdong; Liu, Xia; Jiang, Haiyue

doi:10.1016/j.bioactmat.2022.02.032

Cited by 45 publications

(45 citation statements)

References 58 publications

(71 reference statements)

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“…Current studies on macroporous hydrogels mainly focus on exploring the pluripotency of hydrogel precursors and topological advantages. Due to the risk of foreign body recognition and immune rejection in grafts, autologous biocomponents are an excellent choice for the host or modified macroporous hydrogels [ 242 ]. More autologous bioactive components need to be developed to incorporate macroporous hydrogels to mimic the target ECM more accurately in the future.…”

Section: Discussionmentioning

confidence: 99%

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Wang

et al. 2022

Gels

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Hydrogels have been extensively used as scaffolds in tissue engineering for cell adhesion, proliferation, migration, and differentiation because of their high-water content and biocompatibility similarity to the extracellular matrix. However, submicron or nanosized pore networks within hydrogels severely limit cell survival and tissue regeneration. In recent years, the application of macroporous hydrogels in tissue engineering has received considerable attention. The macroporous structure not only facilitates nutrient transportation and metabolite discharge but also provides more space for cell behavior and tissue formation. Several strategies for creating and functionalizing macroporous hydrogels have been reported. This review began with an overview of the advantages and challenges of macroporous hydrogels in the regulation of cellular behavior. In addition, advanced methods for the preparation of macroporous hydrogels to modulate cellular behavior were discussed. Finally, future research in related fields was discussed.

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Section: Discussionmentioning

confidence: 99%

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Wang

et al. 2022

Gels

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“…By controlling the composition and arrangement of seed cells and carriers to design and produce specific bioactive scaffolds with different structures, full-thickness cartilage that mimics the natural structure of cartilage tissue can be constructed. In a previous study, the cartilage acellular matrix has been successfully prepared into a bioink suitable for bioprinting [ 57 ]. In the following studies, according to the natural fiber distribution in natural cartilage, the geometric modules and unit compactness will be precisely pre-designed to simulate the cartilage micromorphology as perfectly as possible, and finally, the biomimetic structure and mechanical cartilage tissue scaffold will be constructed.…”

Section: Discussionmentioning

confidence: 99%

Acellular cartilage matrix biomimetic scaffold with immediate enrichment of autologous bone marrow mononuclear cells to repair articular cartilage defects

Jia

Zhang

et al. 2022

Materials Today Bio

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“…Its unsatisfactory clinical efficacy is due to reconstruction constructs easily causing inflammation and deformation. As one of the last approaches to solving this issue, Jia et al, used auricular chondrocytes in conjunction with a bioactive bioink based on a biomimetic microporous methacrylate modified acellular cartilage matrix (ACMMA) to produce biological auricle equivalents with precise shapes and low immunogenicity [21]. To date, only six patients have been published with good aesthetic results and without resorption [22].…”

Section: Discussionmentioning

confidence: 99%

Microtia Ear Reconstruction with Patient-Specific 3D Models—A Segmentation Protocol

Arias

Venturini

Martínez

et al. 2022

JCM

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(1) Background: In recent years, three-dimensional (3D) templates have replaced traditional two-dimensional (2D) templates as visual guides during intra-operative carving of the autogenous cartilage framework in microtia reconstruction. This study aims to introduce a protocol of the fabrication of patient-specific, 3D printed and sterilizable auricular models for autogenous auricular reconstruction. (2) Methods: The patient’s unaffected ear was captured with a high-resolution surface 3D scan (Artec Eva) and post-processed in order to obtain a clean surface model (STL format). In the next step, the ear was digitally mirrored, segmented and separated into its component auricle parts for reconstruction. It was disassembled into helix, antihelix, tragus and base and a physical model was 3D printed for each part. Following this segmentation, the cartilage was carved in the operating room, based on the models. (3) Results: This segmentation technique facilitates the modeling and carving of the scaffold, with adequate height, depth, width and thickness. This reduces both the surgical time and the amount of costal cartilage used. (4) Conclusions: This segmentation technique uses surface scanning and 3D printing to produce sterilizable and patient-specific 3D templates.

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Bioprinting and regeneration of auricular cartilage using a bioactive bioink based on microporous photocrosslinkable acellular cartilage matrix

Cited by 45 publications

References 58 publications

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering

Acellular cartilage matrix biomimetic scaffold with immediate enrichment of autologous bone marrow mononuclear cells to repair articular cartilage defects

Microtia Ear Reconstruction with Patient-Specific 3D Models—A Segmentation Protocol

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