Bioprinting a novel glioblastoma tumor model using a fibrin-based bioink for drug screening

Additive Manufacturing of Precision Biomaterials

“…AM or bioprinting offer a complementary approach to fabricate microscale tissue units, as discussed above for large‐scale tissue formation . Recently, bioprinting has been used in parallel to microfabrication to engineer microtissues for in vitro drug screening and disease modeling …”

“…At higher throughput, robotic dispensing has also been applied to model muscle and tendon tissues for improved drug screening and disease modeling . Recently, bioprinting has been adapted to model glioblastoma development, immune cell interactions, and therapy in miniaturized brain models (Figure b) . Laser assisted bioprinting is an additional technology that provides control over the spatial location of cells and materials for microscale tissue fabrication …”

From Dermal Patch to Implants—Applications of Biocomposites in Living Tissues

“…Lee et al [68] developed a fibrin-based biocomposite that was used to create a 3D printed glioblastoma multiforme (GBM) model. The biocomposite ink was composed of fibrin, alginate and genipin.…”

“…Nanohydroxyapatite and glycol chitosan [10] Hydroxyapatite and polymeric blend (fibroin, chitosan and agarose) [11] Calcium silicate, zinc silicate and graphene oxide [15] Collagen, silk fibroin and dECM [26] Boron nitride and boron trioxide [28] Nanohydroxyapatite, calcium sulfate and bioactive molecules [32] Orthopedic Implants PEEK and graphene oxide [39] CFRPEEK, nanohydroxyapatite, carboxymethyl, chitosan and bone forming peptide [41] Polyphenylene sulfide and nanohydroxyapatite [42] Polyimide and tantalum pentaoxide [43] Hydroxyapatite, ceria nanoparticles and silver nanoparticles [44] Wound Healing Polycaprolactone and gelatin [54] Chitosan, polyethylene oxide and fibrinogen [57] Collagen, alginate and silver nanoparticles [60] Polyurethane, keratin and silver nanoparticles [63] Collagen and dextran [65] Tissue Engineering Fibrin, alginate and genipin [68] PEDOT, chitosan and gelatin [70] Polycaprolactone, silk fibroin and carbon nanotubes [71] Silk fibroin and melanin [78] Polycaprolactone and collagen [82] Gelatin, alginate and fibrinogen [88] Collagen type I and gelatin methacryloyl [89] Author Contributions: Conceptualization, K.P.V., A.B., and A.S.; writing-original draft preparation, K.P.V. ; writing-review and editing, K.P.V., A.B., and A.S.; supervision, A.B.…”

“…Applications of Biocomposites to Tissue Engineering: (a) Aspect biosystems' DUO-1TM printhead (top) 3D printed glioblastoma multiforme (GBM) structure (bottom). Reproduced with permission from Reference[68]. (b) Schematic of the synthetic route to the PEDOT/Chitosan/Gelatin scaffold.…”