Bioprinted fibrin-factor XIII-hyaluronate hydrogel scaffolds with encapsulated Schwann cells and their in vitro characterization for use in nerve regeneration

England, Stephanie; Rajaram, Ajay; Schreyer, David J.; Chen, Daniel

doi:10.1016/j.bprint.2016.12.001

Cited by 115 publications

(85 citation statements)

References 61 publications

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“…It is typically mixed with other materials to improve printability, such as gelatin, alginate, or genipin. By extruding fibrinogen mixed with hyaluronic acid and PVA into thrombin solution, fibrin crosslinking was improved and scaffolds with~100 µm features were printed [303]. One advantage of the low viscosity of fibrin is that it can be inkjet-printed.…”

Section: Process Material and Structural Propertiesmentioning

confidence: 99%

“…Fibrin hydrogel encapsulated bone marrow stromal cells were inkjet printed and showed cytocompatibility of the encapsulation material [307]. Fibrin has been used as the base bioink for the printing of neural tissue as a glioblastoma model for drug screening [308]; human dental pulp stem cells for tooth tissue engineering [309]; Schwann cells for nerve tissue engineering [219,303]; human umbilical vein endothelial cells, and hMSCs for bone tissue engineering and neovascularization [310]; as well as human induced pluripotent stem cells for neurological diseases [311].…”

Section: Biocompatibility Biodegradability and Bioactivitymentioning

confidence: 99%

“…Inclusion of genipin, feature size 400 µm, compressive E: 17 kPa to 1.4 MPa [295] Indirect coating of collagen onto TPP scaffold, feature size 30 µm [290] Osteoblast cells, human adipose stem cells [295]; tissue spheroids [296]; keratinocytes and fibroblasts [297], hMSCs [298]; human corneal epithelial cells [257]; osteocytes [300][301][302]; 3D liver microenvironments [209] Fibrin (4.4.2) Mixed with PVA, feature size 100 µm [303] Indirect methods of coating [306], micro-molding with feature size 20 µm [61] Bone marrow stromal cells [307]; neural tissue [308]; dental pulp stem cells [309]; Schwann cells [219,303]; human umbilical vein endothelial cells, hMSCs [310] Gelatin (4.4.3)…”

Section: ) Applicationsmentioning

confidence: 99%

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Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Section: Process Material and Structural Propertiesmentioning

confidence: 99%

Section: Biocompatibility Biodegradability and Bioactivitymentioning

confidence: 99%

Section: ) Applicationsmentioning

confidence: 99%

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Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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“…Extrusion‐based bioprinting is particularly suitable for nerve TE due to the possibility of inducing cell/material alignment along a preferential axis, thus recapitulating the anatomy of neural networks. Along this line, Chen and co‐workers developed a hybrid bioink composed of fibrin, thrombin, hyaluronic acid, and polyvinyl alcohol (HA/PVA) to encapsulate Schwann cells . The anisotropic organization of the resulting 140 mm 3 construct induced preferential cell alignment and elongation of neuronal processes along the fibrin fiber, that is, along the extrusion axis .…”

Section: Macrobioprinting For Large Organ Regenerationmentioning

confidence: 99%

“…Along this line, Chen and co‐workers developed a hybrid bioink composed of fibrin, thrombin, hyaluronic acid, and polyvinyl alcohol (HA/PVA) to encapsulate Schwann cells . The anisotropic organization of the resulting 140 mm 3 construct induced preferential cell alignment and elongation of neuronal processes along the fibrin fiber, that is, along the extrusion axis . Similarly, Owens et al engineered nerve grafts hosting a central lumen to facilitate axon regrowth .…”

Section: Macrobioprinting For Large Organ Regenerationmentioning

confidence: 99%

Micro‐ and Macrobioprinting: Current Trends in Tissue Modeling and Organ Fabrication

2018

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The recapitulation of human anatomy and physiology is critical for organ regeneration. Due to this fundamental requirement, bioprinting holds great promise in tissue engineering and regenerative medicine due to the possibility of fabricating complex scaffolds that host cells and biochemical cues in a physiologically relevant fashion. The ever-growing research in this field has been proceeding along two different, yet complementary, routes: on the one hand, the development of bioprinting to fabricate large tissue surrogates for transplantation purposes in vivo (macrobioprinting), and on the other the spread of bioprinting-based miniaturized systems to model the tissue microenvironment in vitro (microbioprinting). The latest advances in both macro- and microbioprinting are reviewed, emphasizing their impact on specific areas of tissue engineering. Additionally, a critical comparison of macro- versus microbioprinting is presented together with advantages and limitations of each approach. Ultimately, findings obtained both at the macro-and microscale are expected to provide a deeper insight in tissue biology and offer clinically relevant solutions for organ regeneration.

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3D Printed Neural Regeneration Devices

Joung

Lavoie

Guo

et al. 2019

Adv Funct Materials

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Neural regeneration devices interface with the nervous system and can provide flexibility in material choice, implantation without the need for additional surgeries, and the ability to serve as guides augmented with physical, biological (e.g., cellular), and biochemical functionalities. Given the complexity and challenges associated with neural regeneration, a 3D printing approach to the design and manufacturing of neural devices can provide next-generation opportunities for advanced neural regeneration via the production of anatomically accurate geometries, spatial distributions of cellular components, and incorporation of therapeutic biomolecules. A 3D printing-based approach offers compatibility with 3D scanning, computer modeling, choice of input material, and increasing control over hierarchical integration. Therefore, a 3D printed implantable platform can ultimately be used to prepare novel biomimetic scaffolds and model complex tissue architectures for clinical implants in order to treat neurological diseases and injuries. Further, the flexibility and specificity offered by 3D printed in vitro platforms have the potential to be a significant foundational breakthrough with broad research implications in cell signaling and drug screening for personalized healthcare. This progress report examines recent advances in 3D printing strategies for neural regeneration as well as insight into how these approaches can be improved in future studies.spinal cord, and the peripheral nervous system (PNS), composed of the cranial and spinal nerves along with their associated ganglia that connect the CNS to the body. These systems are interconnected via an extensive network of nerves and neural cells (i.e., neurons and supporting glial cells) to facilitate communication and relay information (sensorimotor signals) to and from all parts of the body.

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Bioprinted fibrin-factor XIII-hyaluronate hydrogel scaffolds with encapsulated Schwann cells and their in vitro characterization for use in nerve regeneration

Cited by 115 publications

References 61 publications

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Micro‐ and Macrobioprinting: Current Trends in Tissue Modeling and Organ Fabrication

3D Printed Neural Regeneration Devices

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