Biopolymers/poly(ε-caprolactone)/polyethylenimine functionalized nano-hydroxyapatite hybrid cryogel: Synthesis, characterization and application in gene delivery

Simionescu, Bogdan C.; Drobotă, Mioara; Ţîmpu, Daniel; Vasiliu, Tudor; Constantinescu, Cristina Ana; Rebleanu, Daniela; Calin, Manuela; David, Geta

doi:10.1016/j.msec.2017.07.031

Cited by 22 publications

(12 citation statements)

References 23 publications

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“…Szymonoxicz et al [31] using nHAp in powder form, obtained by the wet chemistry method at temperatures of 800 • C, 900 • C, and 1000 • C, also found that these materials show no cytotoxic effects on fibroblastic cells (L929) [30]. Simionescu et al [32] also verified that biocomposites obtained by combining functionalized nanoparticles of HAp with biopolymers and PCL were non-cytotoxic to human embryonic kidney 293 cells (HEK 293T cells) [32].…”

Section: Discussionmentioning

confidence: 95%

Production and Characterization of Porous Polymeric Membranes of PLA/PCL Blends with the Addition of Hydroxyapatite

et al. 2019

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Polymer membranes have been widely used in guided tissue regeneration (GTR) and guided bone regeneration (GBR). The literature recognizes that poly (lactic acid) (PLA)/poly (ε-caprolactone) (PCL) blends have better physicochemical properties and that a porous polymer surface facilitates cell adhesion and proliferation. In addition, hydroxyapatite (HAp) incorporated into the polymer matrix promotes osteoinduction properties and osteoconduction to the polymer-ceramic biocomposite. Therefore, polymer membranes of PLA/PCL blend with the addition of HAp could be an alternative to be used in GBR. HAp was obtained by precipitation using the mixture of solutions of tetrahydrate calcium nitrate and monobasic ammonium phosphate salts. The porous membranes of the PLA/PCL (80/20) blend with the addition of HAp were obtained by solvent casting with a controlled humidity method, with the dispersion of HAp in chloroform and subsequent solubilization with the components of the blend. The solution was poured into molds for solvent evaporation under a controlled humidity atmosphere. The membranes showed the formation of pores on their surface, together with dispersed HAp particles. The results showed an increase in the surface porosity and improved bioactivity properties with the addition of HAp. Moreover, in biological studies with cell culture, it was possible to observe that the membranes with HAp have no cytotoxic effect on MC3T3 cells. These results indicate a promising use of the new biomaterial for GBR.

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Section: Discussionmentioning

confidence: 95%

Production and Characterization of Porous Polymeric Membranes of PLA/PCL Blends with the Addition of Hydroxyapatite

et al. 2019

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“…The advantage of HA in bone is attributed to its structure and function similar to the mineral composition in bones and teeth. Due to its nano-structure characteristics and chemical affinity to molecules, ions and metal, HA is considered as a delivery system for peptides, proteins, antibiotics, drugs, and genes [59].…”

Section: Hamentioning

confidence: 99%

Local delivery of insulin/IGF-1 for bone regeneration: carriers, strategies, and effects

Zhang¹,

Huan²,

Qi³

et al. 2020

Nanotheranostics

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Bone defects caused by trauma, tumor resection, congenital malformation and infection are still a major challenge for clinicians. Biomimetic bone materials have attracted more and more attention in science and industry. Insulin and insulin-like growth factor-1 (IGF-1) have been increasingly recognized as an inducible factor for osteogenesis and angiogenesis. Spatiotemporal release of insulin may serve as the promising strategy. Considering the successful application of nanoparticles in drug loading, various insulin delivery systems have been developed, including (poly (lactic-co-glycolic acid), PLGA), hydroxyapatite (HA), gelatin, chitosan, alginate, and (γ-glutamic acid)/β-tricalcium phosphate, γ-PGA/β-TCP). Here, we have reviewed the progress on nanoparticles carrying insulin/IGF for bone regeneration. In addition, the key regulatory mechanism of insulin in bone regeneration is also summarized. The future application strategies and the challenges in bone regeneration are also discussed.

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“…A potential silver lining in non-viral vectors research is the use of polycationic macromolecular segments that efficiently complex nucleic acids and gene plasmids into polyplexes while maintaining a good barrier penetration yield [111,112]. Some of the best players in the field, judging by the number of available studies, are linear or branched poly(L-Lysine) (PLL) and poly (ethylene imine) (PEI) [113,114].…”

Section: Polymeric Non-viral Vectors For Gene Deliverymentioning

confidence: 99%

“…The inclusion of such polyplexes into the biocompatible, macroporous matrix of a hybrid cryogel (based on biopolymers, poly(ε-caprolactone, and PEI-functionalized hydroxyapatite) ensures a non-toxic, constant DNA delivery along 26 days and maximum expression in the 5th day [112,119]. Time-dependent assessment of DNA-and vector-release from the 3D matrix evidenced a modulation of the release kinetics based on electrostatic and hydrophobic interplays involving free carrier in excess, polyplexes and the hybrid cryogel, depending on their specific structural features and the degradation of the microporous network.…”

Section: Polymeric Non-viral Vectors For Gene Deliverymentioning

confidence: 99%

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

2020

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Stimuli-responsive, “smart” polymeric materials used in the biomedical field function in a bio-mimicking manner by providing a non-linear response to triggers coming from a physiological microenvironment or other external source. They are built based on various chemical, physical, and biological tools that enable pH and/or temperature-stimulated changes in structural or physicochemical attributes, like shape, volume, solubility, supramolecular arrangement, and others. This review touches on some particular developments on the topic of stimuli-sensitive molecular tools for biomedical applications. Design and mechanistic details are provided concerning the smart synthetic instruments that are employed to prepare supra- and macro-molecular architectures with specific responses to external stimuli. Five major themes are approached: (i) temperature- and pH-responsive systems for controlled drug delivery; (ii) glycodynameric hydrogels for drug delivery; (iii) polymeric non-viral vectors for gene delivery; (iv) metallic nanoconjugates for biomedical applications; and, (v) smart organic tools for biomedical imaging.

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Biopolymers/poly(ε-caprolactone)/polyethylenimine functionalized nano-hydroxyapatite hybrid cryogel: Synthesis, characterization and application in gene delivery

Cited by 22 publications

References 23 publications

Production and Characterization of Porous Polymeric Membranes of PLA/PCL Blends with the Addition of Hydroxyapatite

Production and Characterization of Porous Polymeric Membranes of PLA/PCL Blends with the Addition of Hydroxyapatite

Local delivery of insulin/IGF-1 for bone regeneration: carriers, strategies, and effects

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

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