Biopolymeric Film Containing Bioactive Naphthoquinone (Shikonin) in Combined Therapy of Inflammatory Destructive Lesions in the Buccal Mucosa

Zagorodnyaya, E. B.; Оскольский, Г. И.; Basharov, A. Ya .; Лушникова, Е. Л.; Nepomnyashchikh, L. M.; Zagorodnii, A. S.

doi:10.1007/s10517-013-2318-7

Cited by 13 publications

(5 citation statements)

References 3 publications

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“…Shikonin is also a potent and specific PKM2 inhibitor 21 . It has been demonstrated that shikonin has interesting pharmacological properties, including anti-inflammatory, anticancer, antimicrobial, and wound healing effects 15 , also proven to be safe in clinical trials 31 , 32 . Furthermore, shikonin has the additional effect of antagonizing hepatic, renal, skin and other tissue fibrosis 32 - 34 .…”

Section: Discussionmentioning

confidence: 99%

Integrin β3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis

Mei¹,

Xu²,

Hu³

et al. 2022

Theranostics

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Background: Mechanical ventilation (MV) can induce pulmonary fibrosis. This study aims to investigate whether MV-induced pulmonary fibrosis is associated with aerobic glycolysis and seeks to uncover the underlying mechanisms mediated by integrin β3-pyruvate kinase M2 (PKM2) pathway. Methods: PKM2 knockdown or inhibition, integrin β3 knockout or inhibition and wild-type mice were exposed to MV (20 mL/kg) for 2 h. Results: Mice exposed to MV exhibited increased expression of collagen deposition, and upregulation of α-smooth muscle actin and collagen I in lung tissues. Single cells analysis showed that MV-induced pulmonary fibrosis was associated with increased gene expression of integrin and glycolysis in pulmonary fibroblasts, as well as upregulation of glycolytic products tested by metabolomics. Meanwhile, increased protein level of integrin β3 and PKM2 was confirmed by western blot and immunohistochemistry. Double immunofluorescence staining and flow cytometric analysis showed increased number of fibronectin+/integrin β3+ and fibronectin+/PKM2+ fibroblasts in lung tissues. Furthermore, MV-induced aerobic glycolysis and pulmonary fibrosis were ameliorated after treatment with PKM2 knockdown-AAV and inhibition, or in integrin β3 knockout and inhibition mice. Conclusions : Integrin β3-PKM2 pathway-mediated aerobic glycolysis contributes to MV-induced pulmonary fibrosis. The inhibition of aerobic glycolysis targeting integrin β3-PKM2 pathway may be a promising treatment for MV-induced pulmonary fibrosis.

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Section: Discussionmentioning

confidence: 99%

Integrin β3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis

Mei¹,

Xu²,

Hu³

et al. 2022

Theranostics

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“…En la misma línea, Mansour et al (2014) evaluaron el uso de Aloe vera o Commiphora myrrha en úlceras recurrente oral, reportando que Aloe vera mejoró signos clínicos (eritema, exudado) mientras que el uso de Commiphora myrrha disminuyó la sintomatología dolorosa. Así también, para lesiones de liquen plano erosivo y leucoplasia erosiva, se ha estudiado el uso de Lithospermum erythrorhizon (Zagorodnyaya et al, 2013); para Liquen plano erosivo y no erosivo, el uso de Vitis vinifera (Rivarola de Gutierrez et al, 2014); y para Liquen plano atrófico y erosivo, el uso de Curcuma longa, (Chainani-Wu et al, 2011) reportando todos efectos positivos en los parámetros clínicos de las lesiones y en la sintomatología relatada por los pacientes.…”

Section: Lesiones Orales Ulcerativas (Uro Lpe Lce)unclassified

Fitoterapias en Lesiones de Mucosa Oral: Propiedades Reparativas y Aplicación Clínica. Revisión Sistemática de la Literatura

Fuentes

Faúndez

Roa

2016

Int. J. Odontostomat.

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FAÚNDEZ, F. & ROA, I. Fitoterapias en lesiones de mucosa oral: propiedades reparativas y aplicación clínica. Revisión sistemática de la literatura. Int. J. Odontostomat., 10(3):539-545, 2016. RESUMEN:Dada la exposición constante a injurias, las lesiones de mucosa oral (LMO) y su cicatrización son asunto de importancia para la práctica odontológica, siendo relevante contribuir al restablecimiento de la integridad del área afectada. Se plantea la utilización de fitofármacos como coadyuvantes en la cicatrización de LMO, pero aun cuando los fitofármacos están socialmente valorados, no siempre existe evidencia que los respalde como alternativa terapéutica. Es por esto que consideramos necesario determinar que fitofármacos efectivamente ejercen acción sobre las LMO, para una práctica odontológica basada en evidencia. Se realizó un estudio descriptivo exploratoria pre-sistemático (scoping review), para lo cual se realizaron búsquedas en las bases de datos MEDLINE, Scopus, Web of Science Core Collection, Korean Journal Database, ProQuest Central, SciELO, Biblioteca Cochrane, CUMED, IBECS y LILACS, utilizando los términos MeSH "phytotherapy", "plant extracts", "medicinal plants", "ethnopharmacology", "mouth mucosa" y "oral mucosa". Se incluyeron artículos de los últimos 15 años escritos en español, inglés o portugués, siendo requisito el estudio de heridas o lesiones ulcerativas de la mucosa oral y la evaluación de fitofármacos como alternativa terapéutica para la resolución de las mismas. Resultados: Se seleccionaron mediante lectura de resumen un total de 21 artículos para revisión, correspondiendo 6 a estudios experimentales realizados en animales, 3 a estudios en cultivos celulares, 11 a ensayos clínicos y 1 a revisión Cochrane. Acorde a lo obtenido, los fitofármacos entregan una amplia gama de evidencia de mejorías clínicas e histológicas; existiendo un gran número de artículos científicos que avalan las propiedades benéficas de las plantas y sus componentes activos, sin embargo en cuanto a la cavidad oral, existe un menor número de ensayos clínicos en donde se observen resultados específicos para la mucosa oral. Es necesario ampliar el estudio e inversión en el campo de la fitoterapia, especilamente en lo que concierne a la cicatrización en cavidad oral y sus efectos en la regeneración celular, con el fin de avalar este tipo de terapias.PALABRAS CLAVES: Fitofármacos, fitoterapias, etnofarmacología, cicatrización, mucosa oral. INTRODUCCIÓNLa mucosa oral se encuentra expuesta a constantes noxas, y su capacidad de respuesta está determinada por una serie de eventos destinados a restablecer la integridad del área afectada, proceso conocido como cicatrización (Felzani, 2005). Las lesiones orales se caracterizan por poseer un ciclo de evolución corto, que pueden presentan dolor e incomodidad al paciente, ciclo que puede ser abreviado por la atención médica o si son tratadas (Barrera & Kindelán, 2014).Hoy en día existen diversos métodos, ya sea medicina tradicional o complementaria, que están enfocados a acelerar o mejora...

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“…Shikonin and alkannin, respectively the S‐ and R‐isomers of naphthoquinone, (Figure ) displayed no difference in pharmacological activity to the synthetic product (Kourounakis, Assimopoulou, Papageorgiou, Gavalas, & Kourounakis, ). Shikonin and its derivatives have also been used as natural pigments in cosmetics, as a biological active ingredient in anti‐inflammatories (Lu et al, ), abirritations (Gupta et al, ), antimicrobials (Miao et al, ), and antioxidatives (Z. H. Wang et al, ), as a wound healing agent, and as the main component of Lithospermum oil and film agent, for the treatment of burns (Zagorodnyaya et al, ). Furthermore, shikonin has been reported to have potent antitumor effects against various tumors, including osteosarcoma, leukemia, breast cancer, and carcinoma of salivary gland (Chang, Huang, Chiang, Yeh, & Hsu, ; Mao, Yu, Li, & Li, ; Yao & Zhou, ).…”

Section: Introductionmentioning

confidence: 99%

Evidence for Shikonin acting as an active inhibitor of human carboxylesterases 2: Implications for herb–drug combination

Cao

et al. 2018

Phytotherapy Research

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Shikonin, a natural naphthoquinone compound derived from the herb Lithospermum erythrorhizon, is widely used for its various pharmacological activities. However, its potential interactions with other medications by inhibiting human carboxylesterases 2 (hCE2) remain unknown. In this study, the inhibitory effects of shikonin on the activity of hCE2 in human liver microsomes are investigated by using fluorescein diacetate (FD), N-(2-butyl-1,3-dioxo-2,3-dihydro-1H-phenalen-6-yl)-2-chloroacetamide (NCEN), and CPT-11 as substrates of hCE2. The results demonstrate that shikonin significantly inhibits the activity of hCE2 when FD and NCEN are used as substrates, whereas the half inhibition concentration value of shikonin increased by 5-30 times when CPT-11 was used as the substrate. The inhibition types of shikonin against hCE2 activity reflected by 3 substrates were all best fit to noncompetitive manners. In addition, shikonin was found to distinctly suppress endogenous hCE2 activity, characterized with attenuated fluorescence. Furthermore, for drugs metabolized by hCE2 with the similar binding sites with FD or NCEN, the estimated magnitudes of area under the curve variation were approximately 9-357% in the presence of shikonin. Also, the area under the curve of CPT-11 could be increased by 1-14% following administration of shikonin. These findings have clear clinical implications for the combination of shikonin and hCE2-metabolizing prodrugs.

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Biopolymeric Film Containing Bioactive Naphthoquinone (Shikonin) in Combined Therapy of Inflammatory Destructive Lesions in the Buccal Mucosa

Cited by 13 publications

References 3 publications

Integrin β3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis

Integrin β3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis

Fitoterapias en Lesiones de Mucosa Oral: Propiedades Reparativas y Aplicación Clínica. Revisión Sistemática de la Literatura

Evidence for Shikonin acting as an active inhibitor of human carboxylesterases 2: Implications for herb–drug combination

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