Biopolymer Hydrogel Scaffolds Containing Doxorubicin as A Localized Drug Delivery System for Inhibiting Lung Cancer Cell Proliferation

Abstract

A hydrogel scaffold is a localized drug delivery system that can maintain the therapeutic level of drug concentration at the tumor site. In this study, the biopolymer hydrogel scaffold encapsulating doxorubicin was fabricated from gelatin, sodium carboxymethyl cellulose, and gelatin/sodium carboxymethyl cellulose mixture using a lyophilization technique. The effects of a crosslinker on scaffold morphology and pore size were determined using scanning electron microscopy. The encapsulation efficiency and the release profile of doxorubicin from the hydrogel scaffolds were determined using UV-Vis spectrophotometry. The anti-proliferative effect of the scaffolds against the lung cancer cell line was investigated using an MTT assay. The results showed that scaffolds made from different types of natural polymer had different pore configurations and pore sizes. All scaffolds had high encapsulation efficiency and drug-controlled release profiles. The viability and proliferation of A549 cells, treated with gelatin, gelatin/SCMC, and SCMC scaffolds containing doxorubicin significantly decreased compared with control. These hydrogel scaffolds might provide a promising approach for developing a superior localized drug delivery system to kill lung cancer cells.

Research progress on gel-based nanocomposites for diagnosis and treatment of respiratory diseases

“…These findings suggest that these hydrogel scaffolds may provide a promising method for developing an improved local drug delivery system for eradicating lung cancer cells. 110 Gel materials play a significant role in targeted therapy of lung cancer (Fig. 11).…”

Gene Regulations upon Hydrogel-Mediated Drug Delivery Systems in Skin Cancers—An Overview

“…Unique characteristics of hydrogel such as tunable porous structure, high strength and stimulus response have made them widely used as carriers or vehicles for different biomolecules such as drugs, nucleic acids, antibodies, metal ions and enzymes, and are reported to be effective against different types of cancers such as lung cancer [ 111 ], leukemia [ 112 ], colon cancer [ 113 ], breast cancer [ 114 ], melanoma [ 115 , 116 ], hepatocellular carcinoma [ 116 , 117 ], etc. In addition, it is applied in the delivery of antibodies and other immune modulatory molecules at tumor sites in immunotherapy [ 118 ], drug carriers for the eye [ 119 ] and drug-delivering contact lenses for glaucoma therapy, in tissue engineering and mesenchymal stem/stromal cells [ 120 ].…”