Biophysical regulation of mouse embryonic stem cell fate and genomic integrity by feeder derived matrices

Sthanam, Lakshmi Kavitha; Barai, Amlan; Rastogi, Anuj; Mistari, Vijay Krushna; María, Ana; Kauthale, Rahul R.; Gatne, M. M.; Sen, Shamik

doi:10.1016/j.biomaterials.2016.12.006

Cited by 22 publications

(19 citation statements)

References 59 publications

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“…An advantage of the feeder layer technique demonstrated in our study is that adipogenesis worked efficiently on HFF feeder even when ASCs are seeded at very low density, suggesting that confluent HFFs provide features that support adipogenesis in ASCs. Although further studies are necessary to support this assumption, our data are in keeping with previous findings suggesting that fibroblast feeder monolayers recapitulate biophysical and biochemical features of niche microenvironment [52]. It is well known that the SVF comprises a mixture of different cell types with different phenotypic signatures and differentiation capacity.…”

Section: Discussionsupporting

confidence: 90%

“…In the present study, we employed an HFF feeder monolayer as surrogate for niche cells for human ASCs to mimic both biophysical and biochemical effects of the adipose microenvironment. Although fibroblast or stromal feeder layers represent a widely used model system to provide a supportive cellular microenvironment for stem/progenitor cells [51,52], little is known about the employment of fibroblast feeder layers as niche surrogate for human ASCs. HFFs were used for the feeder layer of human ASCs because they are easily obtained from foreskins and, due to their long replicative lifespan, they are highly expandable [39].…”

Section: Discussionmentioning

confidence: 99%

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Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells

et al. 2019

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Adipose stromal/progenitor cells (ASCs) can differentiate into adipocytes in the course of adipogenesis. This process is governed by systemic factors and signals of the adipose stem cell niche. ASCs isolated from fat tissues and amplified in vitro provide an essential and reliable model system to study adipogenesis. However, current cell culture models routinely grow ASCs on plastic surfaces largely missing niche parameters. In the present communication, we employed human foreskin fibroblasts (HFFs) monolayers as feeder cells for ASCs, which were isolated from human subcutaneous white adipose tissue and amplified in vitro. We found that PPARγ2 and several adipocyte markers were significantly higher expressed in differentiated ASCs growing on feeder layers relative to plastic dishes. Moreover, a significant higher number of adipocytes was generated from ASCs cultured on feeder layer and these adipocytes contained larger fat droplets. Insulin strongly stimulated glucose uptake into adipocytes produced on feeder layer suggesting that these cells show characteristic metabolic features of fat cells. Finally, we show that the HFF feeder layer allows adipogenic differentiation of low-density-seeded ASCs. In conclusion, we demonstrate that the HFF feeder layer increases adipocyte differentiation of ASCs and allows differentiation of low density seeded progenitor cells into functional adipocytes.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells

et al. 2019

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“…Here, we addressed whether tilapia collagen gels that mimic brain stiffness have an effect on the differentiation of pluripotent stem cells. HiPSCs were maintained in plastic dishes without feeder cells to avoid the stiffness effect of feeder-derived matrices 35 . Subsequently, hiPSC-colonies were enzymatically dissociated into single-cell suspensions and seeded at equal densities for passaging either on plastic dishes or on chemically crosslinked gels; the medium was then changed to a neural differentiation medium that has been reported to result in a high rate of production of dorsal cortical neurons 36 .…”

Section: Resultsmentioning

confidence: 99%

Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells

Iwashita

Ohta

Fujisawa

et al. 2019

Sci Rep

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The mechanical properties of the extracellular microenvironment, including its stiffness, play a crucial role in stem cell fate determination. Although previous studies have demonstrated that the developing brain exhibits spatiotemporal diversity in stiffness, it remains unclear how stiffness regulates stem cell fate towards specific neural lineages. Here, we established a culture substrate that reproduces the stiffness of brain tissue using tilapia collagen for in vitro reconstitution assays. By adding crosslinkers, we obtained gels that are similar in stiffness to living brain tissue (150–1500 Pa). We further examined the capability of the gels serving as a substrate for stem cell culture and the effect of stiffness on neural lineage differentiation using human iPS cells. Surprisingly, exposure to gels with a stiffness of approximately 1500 Pa during the early period of neural induction promoted the production of dorsal cortical neurons. These findings suggest that brain-stiffness-mimicking gel has the potential to determine the terminal neural subtype. Taken together, the crosslinked tilapia collagen gel is expected to be useful in various reconstitution assays that can be used to explore the role of stiffness in neurogenesis and neural functions. The enhanced production of dorsal cortical neurons may also provide considerable advantages for neural regenerative applications.

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“…28 Decellularized matrices derived from lung or kidney were reported to promote the commitment of ESCs and iPSCs into epithelial tubules or renal cells. [100][101][102][103] The ECMs derived from the fibroblast feeders were also shown to support the self-renewal of hPSCs during long-term culture, 52,59,86,104 while the ECM of ARPE19 cells increased hPSC differentiation toward retinal pigment epithelial cells. 105 Proteomics analysis identified heparin sulfate proteoglycan as a core ECM component responsible for hPSC self-renewal.…”

Section: Pluripotent Stem Cell Proliferation and Differentiation On Tmentioning

confidence: 97%

“…Recently, the mechanical property of ECMs was found to play a critical role in lineage commitment of stem cells. 52 The increased matrix stiffness (e.g., 34 kPa) was shown to enhance osteogenic differentiation of MSCs, while soft substrates (e.g., 1 kPa) favored chondrogenesis or adipogenesis. [53][54][55] This indicates that MSC differentiation due to mechanical cues (i.e., elastic modulus) primarily corresponds to in vivo localization of the cells.…”

Section: Fig 1 Regulation Of Stem Cell Fate Decisions By Ecm Cues (A)mentioning

confidence: 99%

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

Sart

Jeske

Chen

et al. 2020

Tissue Engineering Part B: Reviews

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Stem cells, including mesenchymal stem cells and pluripotent stem cells, have attracted considerable attention in tissue engineering and regenerative medicine primarily because of their unique ability in self-renewal and multilineage differentiation. However, stem cells also have important secretory functions that form a specialized in vivo microenvironment and direct tissue development and regeneration. Extracellular matrices (ECMs) derived from stem cells retain the functional properties of their native environment and exhibit unique signaling that mediates stem cell self-renewal and lineage commitment. Stem cell-derived ECMs (scECMs) also have tunable properties corresponding to their developmental stages, suggesting that their lineage-and developmental specificity can be engineered for a wide range of applications. Hence, there is a growing interest in reconstructing stem cell microenvironment through decellularization and obtaining decellularized matrices that exhibit unique biological properties. This article summarizes recent advances in the use and understanding of scECMs. Moreover, future directions to extend the spectrum of applications of stem-derived ECMs in tissue engineering by comprehensively elucidating and engineering their regulatory function is highlighted.

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Biophysical regulation of mouse embryonic stem cell fate and genomic integrity by feeder derived matrices

Cited by 22 publications

References 59 publications

Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells

Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells

Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells

Engineering Stem Cell-Derived Extracellular Matrices: Decellularization, Characterization, and Biological Function

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