Biophysical microenvironment and 3D culture physiological relevance

Asthana, Amish; Kisaalita, William S.

doi:10.1016/j.drudis.2012.12.005

Cited by 35 publications

(36 citation statements)

References 74 publications

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“…a hydrogel and clinically relevant human cell type [5]. In addition to therapeutic use in TE, hydrogels as neural scaffolds can also be used for in vitro disease modeling, drug testing and developmental biology studies [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

et al. 2017

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Neural tissue engineering and three-dimensional in vitro tissue modeling require the development of biomaterials that take into account the specified requirements of human neural cells and tissue. In this study, an alternative method of producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with the bioamines spermidine and spermine. These bioamines were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing for the encapsulation of human neurons. We studied the mechanical and rheological properties of the formed hydrogels, which showed biomimicking properties comparable to naïve rabbit brain tissue under physiologically relevant stress and strain. Human pluripotent stem cell-derived neuronal cells demonstrated good cytocompatibility in the GG-based hydrogels. Moreover, functionalization of GG hydrogels with laminin resulted in cell type-specific behavior: neuronal cell maturation and neurite migration.

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Section: Introductionmentioning

confidence: 99%

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

et al. 2017

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“…These results suggest that the stiffness and hydrogel crosslinking density of hydrogel matrix are all important in guiding cell viability and growth, which can be enhanced in a matrix microenvironment that permits the diffusion of both nutrients and wastes (Huang et al, 2011). Such processes are mainly influenced by the permeability and porosity of hydrogel matrix, which can be easily regulated by changing hydrogel crosslinking density (Asthana & Kisaalita, 2013). They subsequently fabricated alginate hydrogel with gradients of RGD peptides (2-18 mg/g).…”

Section: Engineering 3d Cellular Gradient Microenvironmentmentioning

confidence: 96%

Hydrogel-based methods for engineering cellular microenvironment with spatiotemporal gradients

Wang

Huang

et al. 2015

Critical Reviews in Biotechnology

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Natural cellular microenvironment consists of spatiotemporal gradients of multiple physical (e.g. extracellular matrix stiffness, porosity and stress/strain) and chemical cues (e.g. morphogens), which play important roles in regulating cell behaviors including spreading, proliferation, migration, differentiation and apoptosis, especially for pathological processes such as tumor formation and progression. Therefore, it is essential to engineer cellular gradient microenvironment incorporating various gradients for the fabrication of normal and pathological tissue models in vitro. In this article, we firstly review the development of engineering cellular physical and chemical gradients with cytocompatible hydrogels in both two-dimension and three-dimension formats. We then present current advances in the application of engineered gradient microenvironments for the fabrication of disease models in vitro. Finally, concluding remarks and future perspectives for engineering cellular gradients are given.

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“…If the matrix is rigid, it is more difficult for the cells to contract, followed by stress fiber formation in cells and different cell function. Based on their rigidity, 3D materials can be classified into three categories: hydrogels, microporous "spongy" scaffolds and scaffold-free 3D spheroids or cellular aggregates (8).…”

Section: D Cell Culture Technologiesmentioning

confidence: 99%

Three-dimensional cell cultures as a new tool in drug discovery

et al. 2016

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in 3D format will be shown. Conclusions: Although biological significance of obtained data from 2D and 3D cell cultures is still poorly understood, discrepancy of compunds activity illustrated importance of implementation 3D cell culture assays in early part of drug discovery process.IntroductIon T he costs for successfully introduction a NCE (new chemical entity) drug compound to the market is between $800 million and $1,2 billion, with an average duration of about 10 -15 years. Expenses are even much higher in the case of late stage failure (or failure of already marketed drug) (1). Analyses of clinical trials reveal that about 67% of drug lead molecules fail in late clinical trials stages, most of them due to poor efficacy and safety issues. This high attrition rate indicate that current pre-clinical screening and in vitro models do not adequately provide critical information required for prediction of efficacy and safety issues, which remain the main reasons for drug failure (2). The ability of an assay to produce reliable information about tested NCEs is essential in drug development. Therefore, one way to improve drug dis-

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Biophysical microenvironment and 3D culture physiological relevance

Cited by 35 publications

References 74 publications

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering

Hydrogel-based methods for engineering cellular microenvironment with spatiotemporal gradients

Three-dimensional cell cultures as a new tool in drug discovery

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