Biophysical characterization of ovine forestomach extracellular matrix biomaterials

Floden, Evan; Malak, S.; Basil-Jones, Melissa M.; Negron, Leonardo; Fisher, James N.; Lun, S.; Dempsey, Sandi G.; Haverkamp, Richard G.; Ward, Brian R.; May, Barnaby C. H.

doi:10.1002/jbm.b.31740

Cited by 33 publications

(43 citation statements)

References 35 publications

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“…The single-ply lyophilized OFM, used in this study, has relatively low mechanical strength compared to the native tendon (load to failure of 15.07 N and suture pull-out of 5.91 N). However, OFM biomaterial has been fabricated into multi-ply presentations, with load to failures of over 65 N [ 37 ], allowing scope for a more mechanically robust augment through tailoring of the fabrication procedure. However, these were not practical to use in this study due to the relative size of the rat supraspinatus.…”

Section: Discussionmentioning

confidence: 99%

Augmentation with an ovine forestomach matrix scaffold improves histological outcomes of rotator cuff repair in a rat model

et al. 2015

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BackgroundRotator cuff tears can cause significant pain and functional impairment. Without surgical repair, the rotator cuff has little healing potential, and following surgical repair, they are highly prone to re-rupture. Augmenting such repairs with a biomaterial scaffold has been suggested as a potential solution. Extracellular matrix (ECM)-based scaffolds are the most commonly used rotator cuff augments, although to date, reports on their success are variable. Here, we utilize pre-clinical in vitro and in vivo assays to assess the efficacy of a novel biomaterial scaffold, ovine forestomach extracellular matrix (OFM), in augmenting rotator cuff repair.MethodsOFM was assessed in vitro for primary tenocyte growth and adherence, and for immunogenicity using an assay of primary human dendritic cell activation. In vivo, using a murine model, supraspinatus tendon repairs were carried out in 34 animals. Augmentation with OFM was compared to sham surgery and unaugmented control. At 6- and 12-week time points, the repairs were analysed biomechanically for strength of repair and histologically for quality of healing.ResultsOFM supported tenocyte growth in vitro and did not cause an immunogenic response. Augmentation with OFM improved the quality of healing of the repaired tendon, with no evidence of excessive inflammatory response. However, there was no biomechanical advantage of augmentation.ConclusionsThe ideal rotator cuff tendon augment has not yet been identified or clinically implemented. ECM scaffolds offer a promising solution to a difficult clinical problem. Here, we have shown improved histological healing with OFM augmentation. Identifying materials that offset the poorer mechanical properties of the rotator cuff post-injury/repair and enhance organised tendon healing will be paramount to incorporating augmentation into surgical treatment of the rotator cuff.

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Section: Discussionmentioning

confidence: 99%

Augmentation with an ovine forestomach matrix scaffold improves histological outcomes of rotator cuff repair in a rat model

et al. 2015

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“…Type I collagen is also important in products made from animal skin, or related tissues, such as leather and extracellular matrix scaffolds for surgical applications. 1,2 Type II collagen has a fairly similar fibril structure to type I collagen, although with more branching and cross-linking, and is the main structural component of tissues, such as cartilage, therefore parallels may be drawn between type I and type II collagens. The mechanical properties of collagen-based materials are central to the natural and industrial uses of these materials and have been studied in a variety of tissues.…”

Section: Introductionmentioning

confidence: 99%

Poisson's ratio of collagen fibrils measured by small angle X-ray scattering of strained bovine pericardium

Wells

Sizeland

Kayed

et al. 2015

Journal of Applied Physics

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Type I collagen is the main structural component of skin, tendons, and skin products, such as leather. Understanding the mechanical performance of collagen fibrils is important for understanding the mechanical performance of the tissues that they make up, while the mechanical properties of bulk tissue are well characterized, less is known about the mechanical behavior of individual collagen fibrils. In this study, bovine pericardium is subjected to strain while small angle X-ray scattering (SAXS) patterns are recorded using synchrotron radiation. The change in d-spacing, which is a measure of fibril extension, and the change in fibril diameter are determined from SAXS. The tissue is strained 0.25 (25%) with a corresponding strain in the collagen fibrils of 0.045 observed. The ratio of collagen fibril width contraction to length extension, or the Poisson's ratio, is 2.1 ± 0.7 for a tissue strain from 0 to 0.25. This Poisson's ratio indicates that the volume of individual collagen fibrils decreases with increasing strain, which is quite unlike most engineering materials. This high Poisson's ratio of individual fibrils may contribute to high Poisson's ratio observed for tissues, contributing to some of the remarkable properties of collagen-based materials.

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“…31,32 Structural components include elastin, fibronectin, and glycosaminoglycans. Structural studies have shown that CECM biomaterial is relatively strong and elastic and retains the complex collagen architecture of native tissue ECM.…”

Section: Discussionmentioning

confidence: 99%

Leg Ulcer Treatment Outcomes with New Ovine Collagen Extracellular Matrix Dressing

Bohn¹,

Gass²

2014

Advances in Skin &Amp; Wound Care

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Biophysical characterization of ovine forestomach extracellular matrix biomaterials

Cited by 33 publications

References 35 publications

Augmentation with an ovine forestomach matrix scaffold improves histological outcomes of rotator cuff repair in a rat model

Augmentation with an ovine forestomach matrix scaffold improves histological outcomes of rotator cuff repair in a rat model

Poisson's ratio of collagen fibrils measured by small angle X-ray scattering of strained bovine pericardium

Leg Ulcer Treatment Outcomes with New Ovine Collagen Extracellular Matrix Dressing

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