Biopartitioning micellar chromatography: an in vitro technique for predicting human drug absorption

Mixed micellar liquid chromatography methods: modelling quantitative retention–activity relationships of angiotensin converting enzyme inhibitors

“…Table 5 summarizes the statistical analysis of the second-order polynomial models using BMC Brij35 and BMC Brij35:SDS=85:15 systems. In all cases, the equations and statistics for the QRAR models are adjusted to the format recommended by Sagrado and Cronin (Balon et al, 1999;Molero-Monfort et al, 2001;Escuder-Gilabert et al, 2004). The p-values were less than 0.01, which indicated that there were statistically significant relationships between these parameters and log K values obtained in the BMC Brij35 and BMC Brij35:SDS=85:15 systems at the 99% confidence level, for V d , CL and IC50 models with mixed micellar solution at pH 6.5 and 7.4 and the T 1/2 model with the same mobile phase at pH 7.4; the p-values were less than for those models with pure Brij35 solution.…”

“…With amphipathic properties and the capability to mimic the biomembrane, surfactants with unique structures are often used in the field of drug separation and evaluation of drug membranetransport. Biopartitioning micellar chromatography (BMC), an MLC system optimized in order to describe the biological behavior of drugs, comprises a hydrophobic stationary phase and saline solutions of Brij35 micelles as mobile phase, and has been shown to be useful for describing and predicting the biological activities of different pharmacological kinds of drugs (Quiñones-Torrelo et al, 1999Escuder-Gilabert et al, 2000), and permeability across the intestinal barrier (Balon et al, 1999;Molero-Monfort et al, 2001), blood-brain barrier and cornea (Escuder-Gilabert et al, 2004).…”

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…192 Biopartitioning micellar chromatography with polyoxyethylene (23) lauryl ether (Brij35) as the micelle forming agent was used for the prediction of human drug absorption. 193 Calculations based on linear solvation energy relationship were found to be useful to find "open windows" for internal standards in RP-HPLC chromatograms of various compounds, among others steroids 194 and these calculations were used to find suitable internal standard for the determination of estradiol and levonorgestrel in transdermal drug delivery formulations. 195 …”

Imitation of artificial membrane system via mobile phases with Tween-80 and cholic acid in biopartitioning micellar chromatography

“…The membrane proteins stipulate the existence of pores and canals in the structure of membrane, therefore the decomposition of continuity of lipid bilayer takes place, which results in the complication of structure of cell membrane (Pasinsky, 1963;Goodman, 1994). The analogy of surface of cell membrane may be created in the chromatographic column using a mobile phase modified with nonionic surfactant together with C 18 -type stationary phase (Molero-Monfort et al, 2001;Martinez-Pla et al, 2001). The resemblance to the structure of lipid bilayer to erythrocyte and cytoplasmatic membranes may be reinforced by addition of cholic acid to the mobile phase (Kalous and Pavlicek, 1985).…”