Biooxidation of ferrous iron and sulphide at low temperatures: Implications on acid mine drainage and bioleaching of sulphide minerals

Escobar, Beatriz; Buccicardi, S.; Morales, Gladys; Wiertz, Jacques V.

doi:10.1016/j.hydromet.2010.03.027

Cited by 20 publications

(9 citation statements)

References 21 publications

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“…Furthermore, it was documented that a mixed culture was enriched at 5°C with ferrous sulfate as a sole energy and sequences close to Sb. montserratensis were detected at an abundance >10% ( Escobar et al, 2010 ). These results indicated that there might be novel Sulfobacillus species existing in the low-temperature bioleaching culture.…”

Section: Resultsmentioning

confidence: 99%

Bioleaching and Electrochemical Behavior of Chalcopyrite by a Mixed Culture at Low Temperature

et al. 2021

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Low-temperature biohydrometallurgy is implicated in metal recovery in alpine mining areas, but bioleaching using microbial consortia at temperatures <10°C was scarcely discussed. To this end, a mixed culture was used for chalcopyrite bioleaching at 6°C. The mixed culture resulted in a higher copper leaching rate than the pure culture of Acidithiobacillus ferrivorans strain YL15. High-throughput sequencing technology showed that Acidithiobacillus spp. and Sulfobacillus spp. were the mixed culture’s major lineages. Cyclic voltammograms, potentiodynamic polarization and electrochemical impedance spectroscopy unveiled that the mixed culture enhanced the dissolution reactions, decreased the corrosion potential and increased the corrosion current, and lowered the charge transfer resistance and passivation layer impedance of the chalcopyrite electrode compared with the pure culture. This study revealed the mechanisms via which the mixed culture promoted the chalcopyrite bioleaching.

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Section: Resultsmentioning

confidence: 99%

Bioleaching and Electrochemical Behavior of Chalcopyrite by a Mixed Culture at Low Temperature

et al. 2021

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“…In cold environments, like those found at low and high latitudes and at high altitudes, low temperature becomes a limiting factor ( Dopson et al, 2007 ). In Chile currently, there are a number of mining companies located in the Andes Mountains, where the temperature remains near or below 0°C for 6 months or more in the year ( Escobar et al, 2010 ; Gentina and Acevedo, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

et al. 2022

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The bioleaching process is carried out by aerobic acidophilic iron-oxidizing bacteria that are mainly mesophilic or moderately thermophilic. However, many mining sites are located in areas where the mean temperature is lower than the optimal growth temperature of these microorganisms. In this work, we report the obtaining and characterization of two psychrotolerant bioleaching bacterial strains from low-temperature sites that included an abandoned mine site in Chilean Patagonia (PG05) and an acid rock drainage in Marian Cove, King George Island in Antarctic (MC2.2). The PG05 and MC2.2 strains showed significant iron-oxidation activity and grew optimally at 20°C. Genome sequence analyses showed chromosomes of 2.76 and 2.84 Mbp for PG05 and MC2.2, respectively, and an average nucleotide identity estimation indicated that both strains clustered with the acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans. The Patagonian PG05 strain had a high content of genes coding for tolerance to metals such as lead, zinc, and copper. Concordantly, electron microscopy revealed the intracellular presence of polyphosphate-like granules, likely involved in tolerance to metals and other stress conditions. The Antarctic MC2.2 strain showed a high dosage of genes for mercury resistance and low temperature adaptation. This report of cold-adapted cultures of the At. ferrooxidans species opens novel perspectives to satisfy the current challenges of the metal bioleaching industry.

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“…Psychrotolerant bacterium A. ferrivorans has been successfully characterized due to its ability to oxidize Fe 2+ more efficiently than A. ferrooxidans at 5 °C (Kupka et al 2007;Escobar et al 2010;Hallberg et al 2010;Ccorahua-Santo et al 2017). Compared to other Acidithiobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of two novel plasmids from Acidithiobacillus ferrivorans strain PQ33

Ccorahua

Eca

Ramírez³

et al. 2021

Rev peru biol

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Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regulation (CopG), chaperone DnaJ, and a virulence gene (vapD). Furthermore, the plasmids contain sequences similar to phosphate-selective porins O and P and a diguanylate cyclase-phosphodiesterase protein. The presence of these genes suggests the possibility of horizontal transfer, a regulatory system of plasmid maintenance, and adhesion to substrates for A. ferrivorans species and PQ33. This is the first report of plasmids in this strain.

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Biooxidation of ferrous iron and sulphide at low temperatures: Implications on acid mine drainage and bioleaching of sulphide minerals

Cited by 20 publications

References 21 publications

Bioleaching and Electrochemical Behavior of Chalcopyrite by a Mixed Culture at Low Temperature

Bioleaching and Electrochemical Behavior of Chalcopyrite by a Mixed Culture at Low Temperature

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

Comparative genomic analysis of two novel plasmids from Acidithiobacillus ferrivorans strain PQ33

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