Biomolecular evidence of anti-inflammatory effects by Clematis mandshurica Ruprecht root extract in rodent cells

Lee, Chul Won; Park, Sang Mi; Kim, Youn Sook; Jegal, Kyung Hwan; Lee, Jong Rok; Cho, Il Je; Ku, Sae Kwang; Lee, Ji Yeon; Ahn, Yongtae; Son, Yonghae; Ju, Seong A.; Kim, Sang Chan; An, Won Gun

doi:10.1016/j.jep.2014.06.048

Cited by 29 publications

(18 citation statements)

References 53 publications

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“…In macrophages, lipopolysaccharide (LPS), a well-known endotoxin, induces the release of numerous pro-inflammatory mediators such as inducible nitric oxide synthase (iNOS) and inflammatory cytokines, including interleukin-1β (IL-1β) and interleukin-6 (IL-6), which play a major role in the pathogenesis of various inflammatory disorders and serve as significant biomarkers for the assessment of the inflammatory process [3–6]. iNOS is a protein whose expression is regulated by activation of NF-κB, which contribute to the production of NO [7, 8]. Furthermore, mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinases (ERK1/2), c-Jun NH 2 -terminal kinases (JNK) and p38, play important roles in regulation of the inflammatory response by mediators.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas

Zhao

Chen

Deng³

et al. 2017

BMC Complement Altern Med

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Background Ophiopogon japonicas (L.f) Ker-Gawl has been used as a traditional Chinese medicine to cure acute and chronic inflammation and cardiovascular diseases including thrombotic diseases for thousands of years. Previous phytochemical studies showed that O. japonicus contained compounds with anti-inflammatory activity. The aim of this study was to identify and isolate compounds with anti-inflammatory activity from the rhizome of O. japonicas.MethodsCompounds were isolated by various column chromatography and their structures were identified in terms of nuclear magnetic resonance spectrum (NMR) and mass spectrum (MS). To measure the anti-inflammatory effects of thirteen compounds in LPS-induced RAW 264.7 macrophage cells, we used the following methods: cell viability assay, nitric oxide assay, enzyme-linked immunosorbent assay, quantitative real-time PCR analysis and western blotting analysis.ResultsOne new and twelve known compounds (mainly homoisoflavonoids) were extracted from O. japonicas, in which 4′-O-Demethylophiopogonanone E (10) was considered as a new compound, additionally, compounds 4-O-(2-Hydroxy-1- hydroxymethylethyl)-dihydroconiferyl alcohol (2) and 5,7-dihydroxy-6-methyl-3-(2′, 4′-dihydroxybenzyl) chroman-4-one (12) were isolated from the rhizome of O. japonicas for the first time. The isolated compounds Oleic acid (3), Palmitic acid (4), desmethylisoophiopogonone B [5,7-dihydroxy-3-(4′-hydroxybenzyl)-8- methyl- chromone] (5), 5,7-dihydroxy-6-methyl-3-(4′-hydroxybenzyl) chromone (7) and 10 significantly suppressed the production of NO in LPS-induced RAW 264.7 cells. Especially compound 10 showed the strongest effect against the production of the pro-inflammatory cytokine IL-1β and IL-6 with the IC50 value of 32.5 ± 3.5 μg/mL and 13.4 ± 2.3 μg/mL, respectively. Further analysis elucidated that the anti-inflammatory activity of compound 10 might be exerted through inhibiting the phosphorylation of ERK1/2 and JNK in MAPK signaling pathways to decrease NO and pro-inflammatory cytokines production.ConclusionsOur results indicated that 4′-O-Demethylophiopogonanone E can be considered as a potential source of therapeutic medicine for inflammatory diseases.

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Section: Introductionmentioning

confidence: 99%

Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas

Zhao

Chen

Deng³

et al. 2017

BMC Complement Altern Med

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“…Immunoreactivity of the proinflammatory cytokines, IL-1β and TNF-α [60,61], was assessed. Briefly, endogenous peroxidase activity was blocked by incubating tissue sections in methanol and 0.3% H 2 O 2 for 30 min, and non-specific binding of immunoglobulin was blocked with horse serum blocking solution for 1 h in a humidity chamber, after epitope retrieval by pretreatment of trypsin (Sigma-Aldrich, St. Louise, MO, USA) and 2 N HCl on unstained sections, as described previously [52,56].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Anti-Osteoarthritic Effects of a Mixture of Dried Pomegranate Concentrate Powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) in a Surgically Induced Osteoarthritic Rabbit Model

Choi

Kang

Kim³

et al. 2020

Nutrients

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In this study, we aimed to determine the synergistic effects of a formula consisting of dried pomegranate concentrate powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) (PCP:EC:AR) in a surgically induced osteoarthritis (OA) rabbit model. PCP:EC:AR was orally administered once per day. Knee thickness, maximum extension of the knee joint, gross articular defect area, and the histopathological appearance of the cartilage were monitored, along with serum collagen type II C-telopeptide (CTX-II), cartilage oligomeric matrix protein (COMP), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and subchondral IL-1β and TNF-α levels. Roentgenographic images were also evaluated. PCP:EC:AR significantly inhibited the surgically induced increase in knee thickness, maximum extension of both knees, knee thickness after capsule exposure, gross femoral and tibial articular defect areas, loss of the knee joint area, serum and synovial COMP, CTX-II, and MMP expression, and synovial IL-1β, and TNF-α expression. In addition, surgically induced narrowing of the knee bones, loss of the joint area, cartilage damage, and osteophyte formation were reduced. PCP:EC:AR suppressed the surgically induced increases in the Mankin score, and subchondral IL-1β and TNF-α immunolabeled cell numbers. PCP:EC:AR exerted potent OA protective effects in a surgically induced OA rabbit model.

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“…Histological sections were prepared for all sample blocks, which were received as five serial sections in each block. The expression of cyclooxygenase -2 (COX-2) (Cayman, An Arbour, MI, ISA), a key enzyme for the synthesis of prostaglandins, which are chemical mediators of inflammation [25,26] TNF-α (Santa Cruz Biotechnology, Santa Cruz, CA), a pro-inflammatory cytokine [27] , and helper T cell related inflammatory cytokines -IL -4 (Santa Cruz Biotechnology, Santa Cruz, CA), IL-10 (Abcam, Cambridge, UK), and IL 12 [28] on the epidermis and / or dermis of dorsal back skin tissues was evaluated using purified primary antibodies with an ABC and peroxidase substrate kit (Vector Labs, Burlingame, CA, USA), respectively. Briefly, endogenous peroxidase activity was blocked by incubation in methanol and 0.3% H 2 O 2 for 30 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Therapeutic Effects of Bifidobacterium Longum Kacc 91563 on Dncb-Induced Atopic Dermatitis-Like Skin Lesions in Nc / Nga Mice

Lee¹

2018

IJFNS

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Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by severe itch. This study was designed to evaluate the therapeutic effects of oral administration of Bifidobacterium longum, KACC 91563 (BLK) on 2, 4-dinitrochlorobenzene (DNCB) -induced AD-like skin lesions in NC / Nga mice. Three doses of BLK (333, 1667, and 3334 mg / kg / day, 7 times a week) were orally administrated to mice with DNCB-induced AD. The therapeutic effect of BLK was assessed by measuring skin AD score, scratching behavior, serum IgE levels, as well as the expression of COX-2, TNF-α, IL-4, IL-10, and IL-12 by immunohistochemistry. AD score, scratching frequency, serum IgE level, COX-2, TNF-α, IL-4, IL-10, and IL-12 increased in mice with DNCB-induced AD. BLK alleviated AD-like symptoms such as skin severity of AD score and scratching behaviors. In addition, BLK reduced IgE, COX-2, and TNF-α, IL-4, IL-10, and IL-12 expression. These results suggest that oral administration of BLK has beneficial therapeutic effects for AD patients. Citation:Lee, C.K., et al. Therapeutic Effects of Bifidobacterium Longum Kacc 91563 on Dncb-Induced Atopic Dermatitis-Like Skin Lesions in Nc / Nga Mice. (2018) Int J Food Nutr Sci 5(1): 30-37.

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Biomolecular evidence of anti-inflammatory effects by Clematis mandshurica Ruprecht root extract in rodent cells

Cited by 29 publications

References 53 publications

Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas

Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas

Anti-Osteoarthritic Effects of a Mixture of Dried Pomegranate Concentrate Powder, Eucommiae Cortex, and Achyranthis Radix 5:4:1 (g/g) in a Surgically Induced Osteoarthritic Rabbit Model

Therapeutic Effects of Bifidobacterium Longum Kacc 91563 on Dncb-Induced Atopic Dermatitis-Like Skin Lesions in Nc / Nga Mice

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