Biomolecular changes that occur in the antennal gland of the giant freshwater prawn (Machrobrachium rosenbergii)

Abstract

In decapod crustaceans, the antennal gland (AnG) is a major primary source of externally secreted biomolecules, and some may act as pheromones that play a major role in aquatic animal communication. In aquatic crustaceans, sex pheromones regulate reproductive behaviours, yet they remain largely unidentified besides the N-acetylglucosamine-1,5-lactone (NAGL) that stimulates male to female attraction. In this study, we used an AnG transcriptome of the female giant freshwater prawn (Macrobrachium rosenbergii) to predict the secretion of 226 proteins, including the most abundantly expressed transcripts encoding the Spaetzle protein, a serine protease inhibitor, and an arthropodial cuticle protein AMP 8.1. A quantitative proteome analysis of the female AnG at intermolt, premolt and postmolt, identified numerous proteins of different abundances, such as the hemocyanin subunit 1 that is most abundant at intermolt. We also show that hemocyanin subunit 1 is present within water surrounding females. Of those metabolites identified, we demonstrate that the NAGL and N-acetylglucosamine (NAG) can bind with high affinity to hemocyanin subunit 1. In summary, this study has revealed components of the female giant freshwater prawn AnG that are released and contribute to further research towards understanding crustacean conspecific signalling.

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

“…Mating of sex-reversed females (neo-males) with normal females (ZW) could increase a higher ratio of females, while mating of sex-reversed males (neo-females) with normal males could produce all-male (ZZ × ZZ) progeny [1,6,7]. Recently, along with the emergence of high-throughput sequencing, RNA-seq as a most powerful tool is available for illustrating the mechanisms of immune response [35], determining the expression pattern of reproduction, growth, and pheromone communication in hepatopancreas, gill, muscle, and antennal gland [36][37][38]. In the present study, we profiled to detect the transcriptomes of gonads in females, super females, and the males using RNA-seq, with the aim of interpreting the molecular mechanism involved in the sex determination, and identifying sex-related candidate genes.…”

Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

“…Otherwise, limited researches of metabolomics were published in M. rosenbergii. Bose et al conducted untargeted metabolomics of the antennal gland (AnG), and identified several metabolites and biosynthetic pathway implicated in endogenous and exogenous transport [18]. Dong et al performed muscle metabolomics of M. rosenbergii by treating with different concentration of ammonia-N (0, 0.108, 0.324, or 0.54 mg L−1) for 20 days.…”

“…rosenbergii . Bose et al conducted untargeted metabolomics of the antennal gland (AnG), and identified several metabolites and biosynthetic pathway implicated in endogenous and exogenous transport [ 18 ]. Dong et al performed muscle metabolomics of M .…”

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

“…Males grow faster and have larger body size than females, so male prawns are generally preferable to female ones in aquaculture as they can bring more economic benefits (Ventura et al, ; Ventura & Sagi, ). As a result of this, considerable research effort has been devoted to unravel the molecular mechanism of sex determination and differentiation of M. rosenbergii , with the hope of achieving monosex production for this species (Bose et al, ; Jin et al, ; Ventura & Sagi, ). Previous studies have demonstrated that sex differentiation and development of M. rosenbergii is regulated by an endocrine organ unique to decapod crustaceans, namely the androgenic gland (AG) (Jin et al, ; Sharabi et al, ).…”