Biomineralization of magnetic nanoparticles in stem cells

Fromain, Alexandre; Van de Walle, Aurore; Curé, Guilhem; Péchoux, Christine; Serrano, Aida; Lalatonne, Yoann; Espinosa, Ana; Wilhelm, Claire

doi:10.1039/d3nr00863k

Cited by 3 publications

(3 citation statements)

References 51 publications

(71 reference statements)

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“…Besides hydrogel trapping and/or microfluidic constraint, magnetic levitation has been proposed as an interesting alternative since it allows to move cells in all 3 directions (11). However, magnetic levitation requires the magnetization of cells by labeling with nanoparticles and the use of paramagnetic cell culture media, a process that can be problematic for longterm studies (27,28). In this context, acoustic levitation appears to be a promising alternative strategy, as it requires no cell labeling, no modification of the culture medium, and no mechanical contact.…”

Section: Discussionmentioning

confidence: 99%

Microgravity stimulates network activity of 3D neuronal spheroids in an acoustic trap

Pierre-Ewen,

Guillaume,

Chloé

et al. 2024

Preprint

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Among biological models, cell culture constitutes an important paradigm that allows rapid examination of cell phenotype and behavior. While cell cultures are classically grown on a 2D substrate, the recent development of organoid technologies represents a paradigmatic shift in biological experimentation as they pave the way for the reconstruction of minimalist organs in 3D. Manipulating these 3D cell assemblies represents a considerable challenge. While there is growing interest in studying the behavior of cells and organs in the space environment, manipulating 3D cultures in microgravity remains a challenge. But with cellular research underway aboard the International Space Station (ISS), optimizing techniques for handling 3D cellular assemblies is essential. Here, in order to cultivate 3D models of spheroids in microgravity, we developed and used an acoustic bioreactor to trap levitating cellular organoids in a liquid cell culture medium. Indeed, in a Bulk Acoustic Wave (BAW) resonator, spherical objects, such as cells, can be maintained in an equilibrium position, inside a resonant cavity, away from the walls. In the acoustic levitation plane, gravity is counterbalanced by the acoustic radiation force (ARF) making it possible to maintain an object even in weightlessness. A dedicated setup was designed and built to perform live calcium imaging during parabolic flights. During a parabolic flight campaign, we were able to monitor the calcium activity of 3D neural networks trapped in an acoustic field during changes in gravity during different parabolas. Our results clearly indicate a change in calcium activity associated with variations in gravity.

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Section: Discussionmentioning

confidence: 99%

Microgravity stimulates network activity of 3D neuronal spheroids in an acoustic trap

Pierre-Ewen,

Guillaume,

Chloé

et al. 2024

Preprint

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“…It involves a transformation from Fe 2+ to Fe 3+ ions, culminating in the formation of ferrihydrite, alongside a minor magnetic phase. The implications of such biomineralization on cell viability and physiological stability are profound, warranting further exploration [ 269 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Magnetogenetics as a promising tool for controlling cellular signaling pathways

Latypova,

Yaremenko,

Pechnikova

et al. 2024

J Nanobiotechnol

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Magnetogenetics emerges as a transformative approach for modulating cellular signaling pathways through the strategic application of magnetic fields and nanoparticles. This technique leverages the unique properties of magnetic nanoparticles (MNPs) to induce mechanical or thermal stimuli within cells, facilitating the activation of mechano- and thermosensitive proteins without the need for traditional ligand-receptor interactions. Unlike traditional modalities that often require invasive interventions and lack precision in targeting specific cellular functions, magnetogenetics offers a non-invasive alternative with the capacity for deep tissue penetration and the potential for targeting a broad spectrum of cellular processes. This review underscores magnetogenetics’ broad applicability, from steering stem cell differentiation to manipulating neuronal activity and immune responses, highlighting its potential in regenerative medicine, neuroscience, and cancer therapy. Furthermore, the review explores the challenges and future directions of magnetogenetics, including the development of genetically programmed magnetic nanoparticles and the integration of magnetic field-sensitive cells for in vivo applications. Magnetogenetics stands at the forefront of cellular manipulation technologies, offering novel insights into cellular signaling and opening new avenues for therapeutic interventions.

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“…Moreover, under specific conditions, human cells might be able to use the iron ions released over the biodegradation for the production of new magnetic nanoparticles, fully biological 218,219 that appear to arise upon weeks of cell culture. 220 This biomineralization of magnetic nanoparticles could become advantageous for repeated treatment due to their potential long-term persistence. A protective coating avoiding the dissolution of the magnetic core could also be considered.…”

Section: Biocompatibility and Biological Fate Of Magnetic Nanoparticlesmentioning

confidence: 99%

Emergence of magnetic nanoparticles in photothermal and ferroptotic therapies

Van de Walle,

Figuerola,

Espinosa

et al. 2023

Mater. Horiz.

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Biomineralization of magnetic nanoparticles in stem cells

Abstract: Iron is one of the most common metals in the human body, with an intrinsic metabolism including proteins involved in its transport, storage, and redox mechanisms. A less explored singularity...

Cited by 3 publications

References 51 publications

Microgravity stimulates network activity of 3D neuronal spheroids in an acoustic trap

Microgravity stimulates network activity of 3D neuronal spheroids in an acoustic trap

Magnetogenetics as a promising tool for controlling cellular signaling pathways

Emergence of magnetic nanoparticles in photothermal and ferroptotic therapies

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