Biomineralization of electrospun poly(l-lactic acid)/gelatin composite fibrous scaffold by using a supersaturated simulated body fluid with continuous CO2 bubbling

Mineralization on polylactide/gelatin composite nanofibers using simulated body fluid containing amino acid

“…Therefore, it was an effective approach to explain the adsorption effect of organic molecule on the growth of inorganic crystal by adding amino acids into SBFs in vitro. We prepared and used the PLLA/gelatin composite nanofibers as substrates for biomineralizaiton because they were able to nucleate HA on their surfaces by being soaked in SBFs [27,39]. Type B gelatin, whose isoelectric point is pH 4.5-5.5, was used in the present study.…”

“…Herein, nanofibers consisting of poly(l-lactide) (PLLA, 50 wt%) and gelatin (50 wt%) were electrospun and immersed in 2.5 times SBF (2.5SBF) at 37 • C for different times in this study. The non-woven PLLA/gelatin nanofibers were used because they had been proven hydrophilic and able to remain fiber morphology during SBF soaking in our previous work [26,27]. Amino acids (glycine, aspartic acid, or arginine) with different charge characteristics were added into the 2.5SBF to explore their roles in nucleation and transformation of CaP minerals in simulated biomineralization.…”

Preparation of biomimetic hydroxyapatite by biomineralization and calcination using poly(l-lactide)/gelatin composite fibrous mat as template

“…5SBF was prepared as in Ref. [8], in which no buffer was added and only HCl acid was used to adjust the initial solution pH to 6.4. For comparison, a c-5SBF was prepared by additionally using the conventional Tris as the buffer to stabilize the solution pH.…”

“…To mimic the natural mineralization process, polymeric or metallic templates with suitable surface features had been used [7]. In a previous study, the electrospun poly(L-lactide)(PLLA)/gelatin composite fibers were proven good templates to induce mineralizaton [8]. In most cases, however, the deposited mineralites from SBF-soaking were in a sort of flaky-like structure, which was different from that of the natural bone minerals.…”

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

“…Recently, Cai et al reported biomineralization of electrospun poly(L-lactic acid)/ gelatin composite fibrous scaffold by using a supersaturated simulated body fluid with continuous CO 2 bubbling [39]. They found that the mineralites could be formed heterogeneously in the 5Â SBF with CO 2 bubbling.…”