Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells

Abstract

Silver nanoparticles (AgNPs) have been undeniable for its antimicrobial activity while its antitumour potential is still limited. Therefore, the present study focused on determining cytotoxic effects of AgNPs on Michigan cancer foundation-7 (MCF-7) breast cancer cells and its corresponding mechanism of cell death. Herein, the authors developed a bio-reduction method for AgNPs synthesis using actinomycetes isolated from marine soil sample. The isolated strain was identified by 16s ribotyping method and it was found to be . Furthermore, the synthesised AgNPs were characterised by various bio-analytical techniques such as ultraviolet-visible spectrophotometer, atomic force microscopy, transmission electron microscopy, Fourier transform infra-red spectroscopy, and X-ray diffraction. Moreover, the results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay reveals 44.51 µg of AgNPs to have profound inhibition of cancer cell growth; furthermore, the inhibition of MCF-7 breast cancer cell line was found to be dose dependent on treatment with AgNPs. Acridine orange and ethidium bromide double staining methods were performed for cell morphological analysis. The present results showed that biosynthesised AgNPs might be emerging alternative biomaterials for human breast cancer therapy.

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

“…One‐pot synthesis of gold and silver NPs, derived from Olax nana extract, has been shown to exert multiple therapeutic actions . Silver NPs has been shown to be treated for wound healing, active against resistant pathogens, and cancer ,. Zinc oxide NPs from B. megaterium was reported to exert oxidative stress mediated toxicity …”

Extracellular biosynthesis of silver nanoparticles using Streptomyces griseoplanus SAI-25 and its antifungal activity against Macrophomina phaseolina , the charcoal rot pathogen of sorghum

“…LK3 from marine sediments (Karthik et al, 2014), Streptomyces sp. 09 PBT 005 from sugarcane rhizosphere soil (Saravanakumar et al, 2014) and Streptomyces atrovirens from marine sediments (Subbaiya et al, 2017). Streptomyces are still being explored from broad range of environments from farm soil (Baygar and Ugur, 2017;Gupta et al, 2017) to amazon rainforest (Silva-Vinhote et al, 2017) and acid forest (Składanowski et al, 2017), for silver nanoparticles synthesis and also explored for broad range of applications including antipathogenicity, antioxidants and sensors.…”

Anti-cancer green bionanomaterials: present status and future prospects

“…Notably, the exact mechanism of biosynthesis of metal NPs is not well understood yet (25). However, it is believed that some enzymes play a role in bio-reducing metal ions to form metal NPs (26,27). Figure 2 shows the schematic mechanism of microbial-mediated synthesis of metal NPs (11).…”