Biomimetic spinning of silk fibers and in situ cell encapsulation

Cheng, Jie; Park, DoYeun; Jun, Yesl; Lee, Jaeseo; Hyun, Jinho; Lee, Sang Hoon

doi:10.1039/c6lc00488a

Cited by 26 publications

(19 citation statements)

References 39 publications

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“…Among various fiber-form biomaterials 17 – 21 , microfluidic spinning microfibers have been widely used in biomedical applications such as tissue engineering 22 – 25 and wound dressing 26 due to their extraordinary features such as their ability to guide cell growth 27 – 29 , their large surface-to-volume ratios 30 – 32 , and the various ways their surfaces can be modified. Among the diverse approaches for fabricating microfibers, microfluidics has recently attracted much attention because of its advantages in providing simple, rapid, and spatiotemporal control over the composition of the material along the microfiber, and the ability of this method to encapsulate a cell in the microfiber 22 , 24 , 33 , 34 . However, the importance of the mechanical properties of resulting fibers exposed to physiological conditions has been overlooked.…”

Section: Introductionmentioning

confidence: 99%

The use of microfluidic spinning fiber as an ophthalmology suture showing the good anastomotic strength control

Park

Yong

Cho

et al. 2017

Sci Rep

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Adjusting the mechanical strength of a biomaterial to suit its intended application is very important for realizing beneficial outcomes. Microfluidic spinning fiber have been attracting attention recently due to their various advantages, but their mechanical strength has unfortunately not been a subject of concentrated research, and this lack of research has severely limited their applications. In the current work, we showed the mechanical properties of microfibers can be tuned easily and provided a mathematical explanation for how the microfluidic spinning method intrinsically controls the mechanical properties of a microfluidic spinning fiber. But we were also able to adjust the mechanical properties of such fibers in various other ways, including by using biomolecules to coat the fiber or mixing the biomolecules with the primary component of the fiber and by using a customized twisting machine to change the number of single microfiber strands forming the fiber. We used the bundle fiber as an ophthalmology suture that resulted in a porcine eye with a smoother post-operative surface than did a nylon suture. The results showed the possibility that the proposed method can solve current problems of the microfibers in practical applications, and can thus extend the range of applications of these microfibers.

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Section: Introductionmentioning

confidence: 99%

The use of microfluidic spinning fiber as an ophthalmology suture showing the good anastomotic strength control

Park

Yong

Cho

et al. 2017

Sci Rep

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“…The LIVE/DEAD assay confirmed that the encapsulated HepG2 cells kept a high survival rate for more than 7 d . Similarly, Cheng et al introduced a Y‐shaped PDMS‐based microfluidic device to generate cell‐laden hydrogel fibers, using the SF/alginate solution mixed with L929 fibroblast cells as the core flow, the CaCl 2 aqueous solution as the sheath flow (Figure b) . The results of LIVE/DEAD assay showed that cells in the SF/alginate fibers remained alive with a high viability for a long period (>12 d).…”

Section: Microfluidic Spinning Of Nfsmentioning

confidence: 99%

Section: Microfluidic Spinning Of Nfsmentioning

confidence: 99%

Microfluidic Generation of Nanomaterials for Biomedical Applications

Zhao

Bian

Sun

et al. 2019

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As nanomaterials (NMs) possess attractive physicochemical properties that are strongly related to their specific sizes and morphologies, they are becoming one of the most desirable components in the fields of drug delivery, biosensing, bioimaging, and tissue engineering. By choosing an appropriate methodology that allows for accurate control over the reaction conditions, not only can NMs with high quality and rapid production rate be generated, but also designing composite and efficient products for therapy and diagnosis in nanomedicine can be realized. Recent evidence implies that microfluidic technology offers a promising platform for the synthesis of NMs by easy manipulation of fluids in microscale channels. In this Review, a comprehensive set of developments in the field of microfluidics for generating two main classes of NMs, including nanoparticles and nanofibers, and their various potentials in biomedical applications are summarized. Furthermore, the major challenges in this area and opinions on its future developments are proposed.

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“…Despite the existence of many fabrication methods for microfluidic wet spinning devices, none of them can fulfill all the above-mentioned requirements. For instance, soft lithography has been widely used in a laboratory setting for producing microfluidic chips [8][9][10], but involves tedious procedures including mask design and fabrication, plasma bonding, as well as time-consuming slab alignment [11,12]. Alternatively, subtractive manufacturing techniques, such as laser cutting and micro-milling, can shorten the device production procedure [13][14][15][16], but usually require extensive device engineering [14].…”

Section: Introductionmentioning

confidence: 99%

Facile Fabrication of Microfluidic Chips for 3D Hydrodynamic Focusing and Wet Spinning of Polymeric Fibers

et al. 2020

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Microfluidic wet spinning has gained increasing interest in recent years as an alternative to conventional wet spinning by offering higher control in fiber morphology and a gateway for the development of multi-material fibers. Conventionally, microfluidic chips used to create such fibers are fabricated by soft lithography, a method that requires both time and investment in necessary cleanroom facilities. Recently, additive manufacturing techniques were investigated for rapid and cost-efficient prototyping. However, these microfluidic devices are not yet matching the resolutions and tolerances offered by soft lithography. Herein, we report a facile and rapid method using selected arrays of hypodermic needles as templates within a silicone elastomer matrix. The produced microfluidic spinnerets display co-axially aligned circular channels. By simulation and flow experiments, we prove that these devices can maintain laminar flow conditions and achieve precise 3D hydrodynamic focusing. The devices were tested with a commercial polyurethane formulation to demonstrate that fibers with desired morphologies can be produced by varying the degree of hydrodynamic focusing. Thanks to the adaptability of this concept to different microfluidic spinneret designs—as well as to its transparency, ease of fabrication, and cost-efficient procedure—this device sets the ground for transferring microfluidic wet spinning towards industrial textile settings.

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Biomimetic spinning of silk fibers and in situ cell encapsulation

Cited by 26 publications

References 39 publications

The use of microfluidic spinning fiber as an ophthalmology suture showing the good anastomotic strength control

The use of microfluidic spinning fiber as an ophthalmology suture showing the good anastomotic strength control

Microfluidic Generation of Nanomaterials for Biomedical Applications

Facile Fabrication of Microfluidic Chips for 3D Hydrodynamic Focusing and Wet Spinning of Polymeric Fibers

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