Biomimetic mineralized microenvironment stiffness regulated BMSCs osteogenic differentiation through cytoskeleton mediated mechanical signaling transduction

Chen, Lu; Wu, Chengheng; Wei, Dan; Chen, Suping; Xiao, Zhu; Zhu, Hua; Luo, Hongrong; Sun, Jing; Fan, Hongsong

doi:10.1016/j.msec.2020.111613

Cited by 23 publications

(30 citation statements)

References 74 publications

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“… [ 155 ] Stem cells Bone MSCs Enzymatic mineralizable collagen hydrogel 12.9–525.5 kPa To investigate the effect of matrix stiffness on osteogenic differentiation of bone MSCs The mineralized hydrogel with higher stiffness promoted the osteogenic differentiation of MSCs through inducing cytoskeletal assembly, which then enhanced the expression and nuclear colocalization of YAP and RUNX2. [ 156 ] Human MSCs Polyacrylamide hydrogel 1.6–40 kPa To study the effects of matrix elasticity and cell density on human MSCs differentiation Interplays between cell-matrix and cell-cell interactions contributed to MSCs differentiation. The promotion of osteogenic differentiation on hard matrix was mediated through the Ras pathway which is not sufficient for osteogenesis.…”

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

“…On the stiff substrate, MSCs also tend to exhibit a polygonal morphology with a large spreading area, good proliferation [ 160 ], and increased expression of osteogenic differentiation markers through a stiffness-sensing mechanism that is mediated by integrin engagement ( e.g. , α 2 , β 1 , and α 5 β 1 integrin), myosin-based contraction of the cytoskeleton [ 156 ]. Chen et al and Hwang et al have demonstrated that stiffer substrate could significantly promote the osteogenic differentiation of MSCs via increasing the nuclear translocation of YAP and TAZ for cytoskeletal assembly [ 156 , 165 ].…”

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

“…, α 2 , β 1 , and α 5 β 1 integrin), myosin-based contraction of the cytoskeleton [ 156 ]. Chen et al and Hwang et al have demonstrated that stiffer substrate could significantly promote the osteogenic differentiation of MSCs via increasing the nuclear translocation of YAP and TAZ for cytoskeletal assembly [ 156 , 165 ]. To further illustrate the relevant mechanisms, Yuan et al have revealed that MSCs on stiffer substrate could increasingly activate AKT and upregulate expression of YAP target genes and the Runx2 gene.…”

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

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An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

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Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

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An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Liu

2022

Bioactive Materials

123

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“…For example, collagen scaffolds mineralized by PILP using carboxymethyl chitosan as a negatively charged polymer showed a higher modulus of elasticity than scaffolds mineralized traditionally by immersion in SBF [ 30 ]. The improved mechanical properties then had supportive effects on cell adhesion and spreading, on the assembly of the actin cytoskeleton and particularly on osteogenic cell differentiation [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…The osteoconductive and osteoinductive performance of biomineralized scaffolds is therefore better than in scaffolds with artificially admixed mineral fillers (for a review, see [ 29 , 30 , 31 ]). Biomineralized collagen can be processed by advanced techniques, such as 3D printing together with cells [ 10 , 31 ], and can be used for gene delivery [ 32 ] and for osteogenic differentiation of stem cells [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation

et al. 2022

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Collagen, as the main component of connective tissue, is frequently used in various tissue engineering applications. In this study, porous sponge-like collagen scaffolds were prepared by freeze-drying and were then mineralized in a simulated body fluid. The mechanical stability was similar in both types of scaffolds, but the mineralized scaffolds (MCS) contained significantly more calcium, magnesium and phosphorus than the unmineralized scaffolds (UCS). Although the MCS contained a lower percentage (~32.5%) of pores suitable for cell ingrowth (113–357 μm in diameter) than the UCS (~70%), the number of human-osteoblast-like MG-63 cells on days 1, 3 and 7 after seeding was higher on MCS than on UCS, and the cells penetrated deeper into the MCS. The cell growth in extracts prepared by eluting the scaffolds for 7 days in a cell culture medium was also markedly higher in the MCS extracts, as indicated by real-time monitoring in the sensory xCELLigence system for 7 days. From this point of view, MCS are more promising for bone tissue engineering than UCS. However, MCS evoked a more pronounced inflammatory response than UCS, as indicated by the production of tumor necrosis factor-alpha (TNF-α) in macrophage-like RAW 264.7 cells in cultures on these scaffolds.

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Formation Theory and Printability of Photocurable Hydrogel for 3D Bioprinting

Sun

Liu

et al. 2023

Adv Funct Materials

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Bio-ink has gradually transited from ionic-crosslinking to photocrosslinking due to photocurable bio-hydrogel having good formability and biocompatibility. It is very important to understand and quantify the crosslinking process of photocurable hydrogels, otherwise, bioprinting cannot be standardized and scalable. However, there are few studies on hydrogel formation process and its photocrosslinking behavior which cannot be accurately predicted. Herein, the photoinitiated radical polymerized bio-hydrogels are taken as an example to establish the formation theory. Three typical crosslinking reactions are first distinguished. It is further proposed that not all double-bonds consumed during crosslinking contributeequally to polymerization. Then the concept of effective double-bond conversion (EDBC) is elicited. Deriving from EDBC, several important formation indices are defined. According to theory, it is predicted that slow crosslinking can improve the crosslinking degree. Furthermore, based on the slow crosslinking effect, a new strategy of projectionbased 3D printing (PBP) is proposed, which significantly improved printing quality and efficiency. Overall, this work will fill the gap in hydrogel's formation theory, making it possible to accurately quantify the formation process.

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Biomimetic mineralized microenvironment stiffness regulated BMSCs osteogenic differentiation through cytoskeleton mediated mechanical signaling transduction

Cited by 23 publications

References 74 publications

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation

Formation Theory and Printability of Photocurable Hydrogel for 3D Bioprinting

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