Biomimetic mesoporous vectors enabling the efficient inhibition of wild-type isocitrate dehydrogenase in multiple myeloma cells

Cauda, Valentina; Xu, Teng Teng; Nunes, Inês Cristina Camelo; Mereu, Elisabetta; Villata, Simona; Bergaggio, Elisa; Labrador, Maria; Limongi, Tania; Susa, Francesca; Chiodoni, Angelica; Cumerlato, Michela; Rosso, Giada; Stefanìa, Rachele; Piva, Roberto

doi:10.1016/j.micromeso.2021.111320

Cited by 8 publications

(9 citation statements)

References 37 publications

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“…Data are in agreement with previously published works, demonstrating that the lipidic bilayer offers a valid shell to (1) retain the encapsulated molecule and (2) prevent the rapid drug leakage and release from the NP pores, maximizing the drug accumulation in the intracellular compartments. 20,24,26…”

Section: Resultsmentioning

confidence: 99%

“…22 This approach offers several advantages: besides guaranteeing the colloidal stability of nanoparticles in biological media, it increases their biocompatibility and biomimicking features and improves the interaction with the cell membrane, facilitating the endocytosis process. 23 The lipidic bilayer also offers a valid barrier to the rapid drug leakage out of the nanoparticle pores, 20,24 avoiding off-target release, while assuring the drug delivery only intracellularly. Indeed, literature reports have shown the proficient use of lipid bilayer-coated nanoparticles for effective biostabilization in biological media 25 and for efficient cell internalization.…”

Section: Introductionmentioning

confidence: 99%

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Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Sancho-Albero,

Rosso,

De Cola

et al. 2023

Nanoscale

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Sancho-Albero,

Rosso,

De Cola

et al. 2023

Nanoscale

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“…It is then foreseen to load the artificial EVs with active compounds, enabling an advanced function in terms of imaging reporter, diagnostic, or therapeutic activities. Examples of these various functions can derive from the already-reported literature concerning both natural EVs or liposomes, proposing the incorporation of drugs, , genetic materials, organic dyes, aptamers, peptides, and more in general proteins ranging from enzyme to antibodies, and even solid-state nanoparticles of various nature, size, porosities, properties, and functions. − …”

Section: A Novel Practical Strategymentioning

confidence: 99%

Biomimicking Extracellular Vesicles with Fully Artificial Ones: A Rational Design of EV-BIOMIMETICS toward Effective Theranostic Tools in Nanomedicine

Rosso

Cauda

2022

ACS Biomater. Sci. Eng.

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Extracellular Vesicles (EVs) are the protagonists in cell communication and membrane trafficking, being responsible for the delivery of innumerable biomolecules and signaling moieties. At the moment, they are of paramount interest to researchers, as they naturally show incredibly high efficiency and specificity in delivering their cargo. For these reasons, EVs are employed or inspire the development of nanosized therapeutic delivery systems. In this Perspective, we propose an innovative strategy for the rational design of EV-mimicking vesicles (EV-biomimetics) for theranostic scopes. We first report on the current state-of-the-art use of EVs and their byproducts, such as surface-engineered EVs and EV-hybrids, having an artificial cargo (drug molecule, genetic content, nanoparticles, or dye incorporated in their lumen). Thereafter, we report on the new emerging field of EV-mimicking vesicles for theranostic scopes. We introduce an approach to prepare new, fully artificial EV-biomimetics, with particular attention to maintaining the natural reference lipidic composition. We overview those studies investigating natural EV membranes and the possible strategies to identify key proteins involved in site-selective natural homing, typical of EVs, and their cargo transfer to recipient cells. We propose the use also of molecular simulations, in particular of machine learning models, to approach the problem of lipid organization and self-assembly in natural EVs. We also discuss the beneficial feedback that could emerge combining the experimental tests with atomistic and molecular simulations when designing an EV-biomimetics lipid bilayer. The expectations from both research and industrial fields on fully artificial EV-biomimetics, having the same key functions of natural ones plus new diagnostic or therapeutic functions, could be enormous, as they can greatly expand the nanomedicine applications and guarantee ondemand and scalable production, off-the-shelf storage, high reproducibility of morphological and functional properties, and compliance with regulatory standards.

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“…4−6 One of the major trends includes the use of nanosystems incorporating imaging moieties and carrying antitumoral drugs, 7 such as polymeric nanoparticles (NPs), 8−11 liposomes, 12−14 or mesoporous silica NPs. 15,16 However, a main drawback of these devices is the inability to precisely control their release location, leading to an unwanted leakage of their cargo before correctly reaching the tumor environment.…”

Section: Introductionmentioning

confidence: 99%

“…Theranostic nanosystems are acquiring more and more relevance in nanomedicine . The potential advantages offered by the administration of a site-specific therapy while constantly monitoring the patient conditions are actually enormous in the context of cancer, as for all other diseases which are very difficult to treat. , Several prototypes of smart nanosized particles able to perform both these tasks can be found in the literature. − One of the major trends includes the use of nanosystems incorporating imaging moieties and carrying anti-tumoral drugs, such as polymeric nanoparticles (NPs), − liposomes, − or mesoporous silica NPs. , However, a main drawback of these devices is the inability to precisely control their release location, leading to an unwanted leakage of their cargo before correctly reaching the tumor environment.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Phenomena between Iron-Doped ZnO Nanoparticles and Shock Waves Exploited against Pancreatic Cancer Cells

Carofiglio

Conte

Racca

et al. 2022

ACS Appl. Nano Mater.

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We propose the use of iron-doped zinc oxide nanoparticles (Fe:ZnO NPs) showing theranostic capabilities and being synergistically active against pancreatic ductal adenocarcinoma once combined with mechanical pressure waves, such as shock waves. Fe:ZnO NPs are synthesized by employing oleic acid as a capping agent and are functionalized with amino-propyl groups. We first report their superior characteristics with respect to undoped ZnO NPs in terms of magnetic properties, colloidal stability, cytocompatibility, and internalization into BxPC-3 pancreatic cancer cells in vitro. These Fe:ZnO NPs are also cytocompatible toward normal pancreatic cells. We then perform a synergistic cell treatment with both shock waves and Fe:ZnO NPs once internalized into cells. We also evaluate the contribution to the synergistic activity of the NPs located in the extracellular space. Results show that both NPs and shock waves, when administered separately, are safe to cells, while their combination provokes an enhanced cell death after 24 h. Various mechanisms are then considered, such as dissolution of NPs, production of free radicals, and cell membrane disruption or permeation. It is understood so far that iron-doped ZnO NPs can degrade intracellularly into zinc cations, while the use of shock waves produce cell membrane permeabilization and possible rupture. In contrast, the production of reactive oxygen species is here ruled out. The provoked cell death can be recognized in both apoptotic and necrotic events. The proposed work is thus a first proof-of-concept study enabling promising future applications to deep-seated tumors such as pancreatic cancer, which is still an unmet clinical need with a tremendous death rate.

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Biomimetic mesoporous vectors enabling the efficient inhibition of wild-type isocitrate dehydrogenase in multiple myeloma cells

Cited by 8 publications

References 37 publications

Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Biomimicking Extracellular Vesicles with Fully Artificial Ones: A Rational Design of EV-BIOMIMETICS toward Effective Theranostic Tools in Nanomedicine

Synergistic Phenomena between Iron-Doped ZnO Nanoparticles and Shock Waves Exploited against Pancreatic Cancer Cells

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