Biomimetic gold nanoparticles for its cytotoxicity and biocompatibility evidenced by fluorescence-based assays in cancer (MDA-MB-231) and non-cancerous (HEK-293) cells

Jeyarani, Samynathan; Vinita, Nadar Manimaran; Patel, Parimal; Senthamilselvi, S.; Umapathy, Devan; Velangani, Antony Joseph; Biruntha, Muniyandi; Pugazhendhi, Arivalagan; Kumar, Ponnuchamy

doi:10.1016/j.jphotobiol.2019.111715

Cited by 96 publications

(50 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, in our case, the viability of HGFs treated with our heparin-based gold and silver nanoparticles at concentrations up to 25 µg/mL was comparable to that of the control cells (untreated HGFs), supporting the fact that capping agents have to be taken into account when synthesizing nanoparticles for biomedical purposes. Other studies of gold nanoparticles also showed a good preservation of cell viability (about 90%) [60] and significant biocompatibility [61], but the concentrations used were as high as 150 µg/mL, in contrast with the lower concentrations used in the present study.…”

Section: Discussioncontrasting

confidence: 58%

Antifungal and Cytotoxic Evaluation of Photochemically Synthesized Heparin-Coated Gold and Silver Nanoparticles

et al. 2020

View full text Add to dashboard Cite

Heparin-based silver nanoparticles (AgHep-NPs) and gold nanoparticles (AuHep-NPs) were produced by a photochemical method using silver nitrate and chloroauric acid as metal precursors and UV light at 254 nm. UV–Vis spectroscopy graphs showed absorption for AgHep-NPs and AuHep-NPs at 420 nm and 530 nm, respectively. TEM revealed a pseudospherical morphology and a small size, corresponding to 10–25 nm for AgHep-NPs and 1.5–7.5 nm for AuHep-NPs. Their antifungal activity against Candida albicans, Issatchenkia orientalis (Candida krusei), and Candida parapsilosis was assessed by the microdilution method. We show that AgHep-NPs were effective in decreasing fungus density, whereas AuHep-NPs were not. Additionally, the viability of human gingival fibroblasts was preserved by both nanoparticle types at a level above 80%, indicating a slight cytotoxicity. These results are potentially useful for applications of the described NPs mainly in dentistry and, to a lesser extent, in other biomedical areas.

show abstract

Section: Discussioncontrasting

confidence: 58%

Antifungal and Cytotoxic Evaluation of Photochemically Synthesized Heparin-Coated Gold and Silver Nanoparticles

et al. 2020

View full text Add to dashboard Cite

show abstract

“…There have been numerous studies stating on the anticancer properties of AuNPs synthesized through biological approach. More studies about the comparative evaluation of AuNPs against cancer and normal cells are needed for further extended research [16].…”

Section: Limitations In the Application Of Nanomaterialsmentioning

confidence: 99%

Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line

R¹,

Iyer²

2020

Egypt Liver Journal

View full text Add to dashboard Cite

Background: Currently, nanomaterials (NMs) research and development is at a fastest pace due to the enhanced implication in different areas of applications. The synthesis of such NMs through biosynthesis methods is gaining much importance because of low cost involved and environment-friendly approach. In this present study, the nanoparticles (NPs) are synthesized using a medicinal plant, with anticancer properties so as to incorporate the therapeutic activity within the NPs, such that the NPs will have the attributes of NMs alongside the phytoactivity. Results: The synthesis of gold nanoparticles (AuNPs) using Graviola, the fruit of Annona muricata (Ramaphal fruit) commonly referred as Mullu seetha fruit (Tamil), was successfully carried out. The initial confirmations of the NPs were using UV-vis spectra, which showed the characteristic peak for the NPs. HRSEM analysis gave an insight on the size and morphology of the NPs. The zeta potential was measured to check the stability of the NPs. The cytotoxicity was carried out in VERO cell line and anticancer study in Hep2 liver cancer cell line. The surface plasmon resonance (SPR) band showed the characteristic peak at 536 nm for the AuNPs. In SEM micrographs, the size was ranging between 20 and 30 nm, on an average of 15 nm with spherical morphology. On the various tested concentrations in VERO cell line, the nanoparticles were non-toxic to the cells. The anticancer study gave an IC50 value at 10.94 μg/ml. Conclusions:The NPs showed anticancer activity in treated Hep2 liver cancer cell line and as well as commendable non-toxic effect on normal VERO cell line. The results pose a positive impact to expand further studies in the development of potential drug molecules to tackle the disease of interest.

show abstract

“…Transition metals and their compounds, for example, silver and silver salts, are among the most studied alternatives to fight sensitive and resistant bacteria 6,7 . Metal nanoparticles, especially silver nanoparticles, have been used for a variety of biomedical applications 8 like antimicrobial, antibiofilm activity, larvicidal and insecticidal effects, and anticancer activity [9][10][11][12] . Nevertheless, the use of such metals in therapeutic agents is limited by their toxicity on eukaryotic cells.…”

mentioning

confidence: 99%

Antimicrobial activity of a silver-microfibrillated cellulose biocomposite against susceptible and resistant bacteria

Garza-Cervantes

Mendiola-Garza

Melo

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Antibiotic Microbial Resistance (AMR) is a major global challenge as it constitutes a severe threat to global public health if not addressed. To fight against AMR bacteria, new antimicrobial agents are continually needed, and their efficacy must be tested. Historically, many transition metals have been employed, but their cytotoxicity is an issue and hence must be reduced, typically by combination with organic polymers. Cellulose of natural origin, especially those derived from unavoidable residues in the food supply chain, appears to be a good capping agent for the green synthesis of silver nanoparticles. Herein, we describe a green synthesis method to produce a novel biocomposite, using ascorbic acid as reducing agent and microfibrillated cellulose as a capping agent and demonstrate this material to be an efficient antimicrobial agent. Silver nanoparticles were obtained in the cellulose matrix with an average size of 140 nm and with antimicrobial activity against both sensitive and resistant Gram positive (using 1500 ppm) as well as sensitive and resistant Gram negative (using 125 ppm) bacteria. Also, an inverted disk-diffusion methodology was applied to overcome the low-solubility of cellulose compounds. This novel silver nanoparticle-cellulose biocomposite synthesized by a green methodology shows the potential to be applied in the future development of biomedical instruments and therapeutics.

show abstract

Biomimetic gold nanoparticles for its cytotoxicity and biocompatibility evidenced by fluorescence-based assays in cancer (MDA-MB-231) and non-cancerous (HEK-293) cells

Cited by 96 publications

References 23 publications

Antifungal and Cytotoxic Evaluation of Photochemically Synthesized Heparin-Coated Gold and Silver Nanoparticles

Antifungal and Cytotoxic Evaluation of Photochemically Synthesized Heparin-Coated Gold and Silver Nanoparticles

Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line

Antimicrobial activity of a silver-microfibrillated cellulose biocomposite against susceptible and resistant bacteria

Contact Info

Product

Resources

About