Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line

Abstract

Background: Currently, nanomaterials (NMs) research and development is at a fastest pace due to the enhanced implication in different areas of applications. The synthesis of such NMs through biosynthesis methods is gaining much importance because of low cost involved and environment-friendly approach. In this present study, the nanoparticles (NPs) are synthesized using a medicinal plant, with anticancer properties so as to incorporate the therapeutic activity within the NPs, such that the NPs will have the attributes of NMs alongside the phytoactivity. Results: The synthesis of gold nanoparticles (AuNPs) using Graviola, the fruit of Annona muricata (Ramaphal fruit) commonly referred as Mullu seetha fruit (Tamil), was successfully carried out. The initial confirmations of the NPs were using UV-vis spectra, which showed the characteristic peak for the NPs. HRSEM analysis gave an insight on the size and morphology of the NPs. The zeta potential was measured to check the stability of the NPs. The cytotoxicity was carried out in VERO cell line and anticancer study in Hep2 liver cancer cell line. The surface plasmon resonance (SPR) band showed the characteristic peak at 536 nm for the AuNPs. In SEM micrographs, the size was ranging between 20 and 30 nm, on an average of 15 nm with spherical morphology. On the various tested concentrations in VERO cell line, the nanoparticles were non-toxic to the cells. The anticancer study gave an IC50 value at 10.94 μg/ml. Conclusions:The NPs showed anticancer activity in treated Hep2 liver cancer cell line and as well as commendable non-toxic effect on normal VERO cell line. The results pose a positive impact to expand further studies in the development of potential drug molecules to tackle the disease of interest.

Apoptotic and Antioxidant Activity of Gold Nanoparticles Synthesized Using Marine Brown Seaweed: An In Vitro Study

“…The formation of AuNPs was confirmed by the development of colour which is characteristic of AuNPs. The confirmation was done by recording the UV-Vis absorbance was at 350-650 nm which corroborated with the earlier published results [14,32] in which the UV-Vis spectral analysis of AuNP absorbance was measured at 520-560 nm (27). They confirmed the peak around 540 nm and a broader peak between 700 and 800 nm.…”

“…Despite possessing significant advantages, use of AuNPs has its own limitation due to application of various hazardous chemical synthesis methods for preparation. Hence, green synthesis method using plants, microbes, seaweeds, and its respective bioactive metabolites is used to synthesize AuNPs [ 14 ].…”

Development and optimization of curcumin analog nano-bilosomes using 21.31full factorial design for anti-tumor profiles improvement in human hepatocellular carcinoma:in-vitroevaluation,in-vivosafety assay

“…Also, they increase the longevity of vesicles in circulation (Borborema et al., 2016 ). It was reported that nanocarriers smaller than 500 nm can penetrate the bloodstream efficiently (Priya & Iyer, 2020 ). ZP is an important indicator of formulation stability as a higher surface charge produces repulsive forces that prevent particle coalescence and aggregation (Ahmed et al., 2020 ).…”

A study on ER stress-induced apoptosis pathway in cervical cancer HeLa cells treated with biosynthesized gold nanoparticles

“…The optimization of the underlying parameters such as temperature and pH was carried out, which played crucial role in the synthesis (Kamala Priya and Iyer 2014b). The applications of gold nanoparticles (Kamala Priya and Iyer 2015b), in vitro cytotoxicity (Kamala Priya and Iyer 2019a) and anticancer assay (Kamala Priya and Iyer 2014c, 2019b, 2020a and anti-HIV assay (Priya et al 2021) were carried out in different cell lines. The results demonstrated non-toxicity of the nanomaterials to normal cell line.…”