Biomimetic Electrospun PLLA/PPSB Nanofibrous Scaffold Combined with Human Neural Stem Cells for Spinal Cord Injury Repair

Dai, Yuan; Wang, Weizhong; Zhou, Xiaojun; li, Linli; Tang, Yuyi; Shao, Minghao; Lyu, Feizhou

doi:10.1021/acsanm.3c00374

Cited by 5 publications

(1 citation statement)

References 89 publications

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“…Nanofibers are attractive as drug depots for astrocytes because their intrinsic material properties can alter astrocyte activation or direct astrocyte growth ( Zuidema et al, 2014 , 2018 ). Improved astrocyte activation outcomes have been demonstrated by employing nanofiber scaffolds alone, incorporating stem cells with nanofibers, and even with conductive nanofibers ( Zhao et al, 2018 ; Shu et al, 2019 ; Yan et al, 2020 ; Dai et al, 2023 ; Xu et al, 2023 ). As a means to further promote neural repair and mitigation of secondary injury, nanofibers can be loaded with APIs that act on astrocytes due to their porous nature ( Zhang et al, 2021 ).…”

Section: Nanomaterials For Api Delivery To Cns Gliamentioning

confidence: 99%

Nanomaterial payload delivery to central nervous system glia for neural protection and repair

Saksena,

Hamilton,

Gilbert

et al. 2023

Front. Cell. Neurosci.

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Central nervous system (CNS) glia, including astrocytes, microglia, and oligodendrocytes, play prominent roles in traumatic injury and degenerative disorders. Due to their importance, active pharmaceutical ingredients (APIs) are being developed to modulate CNS glia in order to improve outcomes in traumatic injury and disease. While many of these APIs show promise in vitro, the majority of APIs that are systemically delivered show little penetration through the blood–brain barrier (BBB) or blood-spinal cord barrier (BSCB) and into the CNS, rendering them ineffective. Novel nanomaterials are being developed to deliver APIs into the CNS to modulate glial responses and improve outcomes in injury and disease. Nanomaterials are attractive options as therapies for central nervous system protection and repair in degenerative disorders and traumatic injury due to their intrinsic capabilities in API delivery. Nanomaterials can improve API accumulation in the CNS by increasing permeation through the BBB of systemically delivered APIs, extending the timeline of API release, and interacting biophysically with CNS cell populations due to their mechanical properties and nanoscale architectures. In this review, we present the recent advances in the fields of both locally implanted nanomaterials and systemically administered nanoparticles developed for the delivery of APIs to the CNS that modulate glial activity as a strategy to improve outcomes in traumatic injury and disease. We identify current research gaps and discuss potential developments in the field that will continue to translate the use of glia-targeting nanomaterials to the clinic.

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Section: Nanomaterials For Api Delivery To Cns Gliamentioning

confidence: 99%

Nanomaterial payload delivery to central nervous system glia for neural protection and repair

Saksena,

Hamilton,

Gilbert

et al. 2023

Front. Cell. Neurosci.

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Electrospun Composite PLLA‐PPSB Nanofiber Nerve Conduits for Peripheral Nerve Defects Repair and Regeneration

Dai,

Lu,

et al. 2024

Adv Healthcare Materials

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Peripheral nerve injury (PNI) is a common clinical problem and regenerating peripheral nerve defects remains a significant challenge. Poly(polyol sebacate) (PPS) polymers have been developed as promising materials for biomedical applications due to their biodegradability, biocompatibility, elastomeric properties, and ease of production. However, the application of PPS‐based biomaterials in nerve tissue engineering, especially in PNI repair, is limited. In this study, we aimed to construct PPS‐based composite nanofibers poly(l‐lactic acid)‐poly(polycaprolactone triol‐co‐sebacic acid‐co‐N,N‐bis(2‐hydroxyethyl)‐2‐aminoethanesulfonic acid sodium salt) (PLLA‐PPSB) through electrospinning and assess their in vitro biocompatibility with Schwann cells (SCs) and in vivo repair capabilities for peripheral nerve defects. For the first time, the biocompatibility and bioactivity of PPS‐based nanomaterial were examined at the molecular, cellular, and animal levels for PNI repair. Electrospun PLLA‐PPSB nanofibers displayed favorable physicochemical properties and biocompatibility, providing an effective interface for the proliferation, glial expression, and adhesion of SCs in vitro. In vivo experiments using a 10‐mm rat sciatic nerve defect model showed that PLLA‐PPSB nanofiber nerve conduits enhanced myelin formation, axonal regeneration, angiogenesis, and functional recovery. Transcriptome analysis and biological validation indicated that PLLA‐PPSB nanofibers might promote SC proliferation by activating the PI3K/Akt signaling pathway. This suggests the promising potential of PLLA‐PPSB nanomaterial for PNI repair.This article is protected by copyright. All rights reserved

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Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

Ozcicek,

Aysit,

Balcikanli

et al. 2024

Macromolecular Bioscience

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Spinal cord injuries are very common worldwide, leading to permanent nerve function loss with devastating effects in the affected patients. The challenges and inadequate results in the current clinical treatments are leading scientists to innovative neural regenerative research. Advances in nanoscience and neural tissue engineering have opened new avenues for SCI treatment. In order for designed nerve guidance conduit to be functionally useful, it must have ideal scaffold properties and topographic features that promote the linear orientation of damaged axons. In this study, we aimed to develop channeled polycaprolactone (PCL)/Poly‐D,L‐lactic‐co‐glycolic acid (PLGA) hybrid film scaffolds, modify their surfaces by IKVAV pentapeptide/gold nanoparticles (AuNPs) or polypyrrole (PPy) and investigate the behavior of motor neurons on the designed scaffold surfaces in vitro under static/bioreactor conditions. We also examined their potential to promote neural regeneration after implantation into the rat spinal cord injury by shaping the film scaffolds modified with neural factors into a tubular form. We show that channeled groups decorated with AuNPs highly promote neurite orientation under bioreactor conditions and also the developed optimal nerve guidance conduit (PCL/PLGA G1‐IKVAV/BDNF/NGF‐AuNP50) highly regenerates spinal cord injury. The results indicated that the designed scaffold could be an ideal candidate for spinal cord regeneration.This article is protected by copyright. All rights reserved

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Biomimetic Electrospun PLLA/PPSB Nanofibrous Scaffold Combined with Human Neural Stem Cells for Spinal Cord Injury Repair

Cited by 5 publications

References 89 publications

Nanomaterial payload delivery to central nervous system glia for neural protection and repair

Nanomaterial payload delivery to central nervous system glia for neural protection and repair

Electrospun Composite PLLA‐PPSB Nanofiber Nerve Conduits for Peripheral Nerve Defects Repair and Regeneration

Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

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