Biomimetic copper single-atom nanozyme system for self-enhanced nanocatalytic tumor therapy

Hybrid biomimetic assembly enzymes based on ZIF-8 as “intracellular scavenger” mitigating neuronal damage caused by oxidative stress

“…The SOD@ZIF-8 composites through biomimetic mineralization was constructed according to previous reports ( Fan et al, 2021 ; Zhu et al, 2022 ). Briefly, 2 ml of 2‐methylimidazole (HMeIM) solution (15.68 mg/ml) was mixed with 2 ml of Zn(OAc) 2 solution (8.76 mg/ml), 200 μL of methanol and 1 ml of SOD solution (1 mg/ml).…”

“…These enzyme-MOF composites are able to solve the unfavorable solvent effect of enzymatic structure and limited loading of large enzyme into MOF pores. Unlike other encapsulation approaches such as coprecipitation, enzyme molecules can trigger nucleation of porous crystals by concentrating the MOF building blocks in the process of biomimetic mineralization ( Xie et al, 2010 ; He et al, 2016 ; Jiang et al, 2017 ; Zhu et al, 2022 ). Notably, enzyme-MOF composites obtained from this method can enlarge the bioactive temperature range of enzyme and enhance the stability in a facile manner, demonstrating a great promise in the biomedical application ( Montanari et al, 2012 ; Tabrez et al, 2012 ; Zhu et al, 2012 ; Li et al, 2020 ; Yang et al, 2020 ).…”

Nanocarriers for intracellular co-delivery of proteins and small-molecule drugs for cancer therapy

“…However, it is difficult to guarantee the effective rate and activity release of small molecular drugs and proteins to the tumor site. Recently, it has been reported that nanocarriers are modified by cell membranes or exosomes, these functionalized and biologically recognized nanocarriers greatly improve the membrane permeability and bioavailability, which also provides an idea for the design of co-delivery carriers for proteins and small-molecule drugs ( Fu et al, 2020 ; Zhu et al, 2022 ).…”

CuS nanoparticles and camptothecin co-loaded thermosensitive injectable hydrogel with self-supplied H2O2 for enhanced chemodynamic therapy

“…Further, CDT inhibits energy loss during therapy since it does not require sufficient oxygen (O 2 ) or external energy input ( Feng et al, 2020 ). However, the insufficient concentration of H 2 O 2 in the tumor microenvironment severely limits the therapeutic effect of chemokinetic ( Zhou et al, 2021 ; Zhu et al, 2022c ). At the same time, CDT alone does not effectively trigger immunological responses ( Zhu et al, 2022b ), therefore, it is very necessary to achieve high expression of H 2 O 2 in the tumor microenvironment through a safe and effective method, and to promote the therapeutic effect of CDT through combined therapy, such as radiotherapy and photothermal therapy.…”