Biomimetic coating with phosphoserine‐tethered poly(epsilon‐lysine) dendrons on titanium surfaces enhances Wnt and osteoblastic differentiation

Galli, Carlo; Piemontese, Marilina; Meikle, Steve; Santin, Matteo; Macaluso, Guido Maria; Passeri, Giovanni

doi:10.1111/clr.12075

Cited by 18 publications

(15 citation statements)

References 31 publications

(31 reference statements)

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“…To conclude, findings of this study shows that G3 PSL-PL can act as stimulation for initiation of osteogenic signalling cascades in-vitro and stimulate differentiation of mesenchymal cells into osteoblasts. Thus, in vivo testing might help to use these films as novel coatings to enhance the process of osseointegration and prevent implant failures [106].…”

Section: Methodsmentioning

confidence: 99%

The potential of dendrimer in delivery of therapeutics for dentistry

Bapat

Dharmadhikari

Chaubal

et al. 2019

Heliyon

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Dendrimers are hyperbranched nanoparticle structures along with its surface modifications can to be used in dental biomaterials for biomimetic remineralisation of enamel and dentin. The review highlights the therapeutic applications of dendrimers in the field of dentistry. It addresses the possible mechanisms of enhancement of mechanical properties of adhesives and resins structure. Dendrimers due to its unique construction of possessing inner hydrophobic and outer hydrophilic structure can act as drug carrier for delivery of antimicrobial drugs for treatment of periodontal diseases and at peripheral dental implant areas. Dendrimers due to its hyperbranched structures can provides a unique drug delivery vehicle for delivery of a drug at specific site for sustained release for therapeutic effects. Thus, dendrimers can be one of the most important constituents which can be incorporated in dental biomaterials for better outcomes in dentistry.

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Section: Methodsmentioning

confidence: 99%

The potential of dendrimer in delivery of therapeutics for dentistry

Bapat

Dharmadhikari

Chaubal

et al. 2019

Heliyon

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show abstract

“…It also stimulates its downstream pathways that result in affecting several osteogenic processes including not only osteoblast attachment and differentiation but also cell maturation and apoptosis [ 48 ]. Moreover, the pathway has also been reported to promote both mineral deposition in ECM and the repair of tissue defect [ 49 , 50 ]. The proteins of β-Catenin and Gsk3β are key proteins in the canonical Wnt/β-catenin pathway.…”

Section: Discussionmentioning

confidence: 99%

Lanthanum phosphate/chitosan scaffolds enhance cytocompatibility and osteogenic efficiency via the Wnt/β-catenin pathway

Zhao

Liu

et al. 2018

J Nanobiotechnol

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BackgroundFabrication of porous scaffolds with great biocompatibility and osteoinductivity to promote bone defect healing has attracted extensive attention.MethodsIn a previous study, novel lanthanum phosphate (LaPO4)/chitosan (CS) scaffolds were prepared by distributing 40- to 60-nm LaPO4 nanoparticles throughout plate-like CS films.ResultsInterconnected three dimensional (3D) macropores within the scaffolds increased the scaffold osteoconductivity, thereby promoting cell adhesion and bone tissue in-growth. The LaPO4/CS scaffolds showed no obvious toxicity and accelerated bone generation in a rat cranial defect model. Notably, the element La in the scaffolds was found to promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through the Wnt/β-catenin signalling pathway and induced high expression of the osteogenesis-related genes alkaline phosphatase, osteocalcin and Collagen I (Col-I). Moreover, the LaPO4/CS scaffolds enhanced bone regeneration and collagen fibre deposition in rat critical-sized calvarial defect sites.ConclusionThe novel LaPO4/CS scaffolds provide an admirable and promising platform for the repair of bone defects.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0411-9) contains supplementary material, which is available to authorized users.

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“…To achieve this goal, it has been proposed to coat biomaterials with molecular cues that are specific to cell adhesion or tissue repair. 19 The ECM is the three-dimensional scaffold that contains cells and supplies biochemical signals that are essential to regulate their behavior and function. [20][21][22][23] The ECM is composed of structural proteins such as collagen, and signaling molecules such as growth factors sequestered in the matrix.…”

Section: Discussionmentioning

confidence: 99%

“…Endosseous biomaterials must integrate into healing bone and promote the formation of sound tissue in contact with the implanted device. To achieve this goal, it has been proposed to coat biomaterials with molecular cues that are specific to cell adhesion or tissue repair . The ECM is the three‐dimensional scaffold that contains cells and supplies biochemical signals that are essential to regulate their behavior and function .…”

Section: Discussionmentioning

confidence: 99%

Periostin improves cell adhesion to implantable biomaterials and osteoblastic differentiation on implant titanium surfaces in a topography-dependent fashion

Galli

Piergianni

Piemontese

et al. 2013

J. Biomed. Mater. Res.

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Periostin is a matricellular protein highly expressed in periodontal ligament and periostium and has been shown to be required for tissue development and maintenance. We showed that the adhesion of murine osteoblastic MC3T3 cells to thiolated hyaluronic acid/polyethyleneglycol hydrogels was greatly improved by enrichment with periostin. Polished or sand-blasted/acid-etched (SLA) commercially pure titanium surfaces were also coated with this protein and periostin ameliorated cell adhesion and dramatically affected cell morphology on both surfaces, as assessed at fluorescence microscopy, scanning electron microscopy, and chemiluminescence-based viability assay. Moreover, periostin increased the expression of alkaline phosphatase, osteoprotegerin, connective tissue growth factor, collagen 1a1, osteocalcin, Runx2, and osterix transcription factors on smooth surfaces. However, it did not affect, or even decreased, the expression of these genes on SLA discs. Transcript levels for connexin 43 were greatly increased on both surfaces in the presence of periostin. Taken together, these results show that periostin coatings can be a viable approach to improve cell adhesion and differentiation on implantable biomaterials.

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Biomimetic coating with phosphoserine‐tethered poly(epsilon‐lysine) dendrons on titanium surfaces enhances Wnt and osteoblastic differentiation

Abstract: The findings of the present study show that phosphoserine-tethered poly(epsilon-lysine) dendron films act as stimuli for the activation of specific signal cascades and promote the differentiation of adhering progenitor cells into an osteoblastic phenotype.

Cited by 18 publications

References 31 publications

The potential of dendrimer in delivery of therapeutics for dentistry

The potential of dendrimer in delivery of therapeutics for dentistry

Lanthanum phosphate/chitosan scaffolds enhance cytocompatibility and osteogenic efficiency via the Wnt/β-catenin pathway

Periostin improves cell adhesion to implantable biomaterials and osteoblastic differentiation on implant titanium surfaces in a topography-dependent fashion

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