Biomimetic Chemistry: Radical Reactions in Vesicle Suspensions

Chatgilialoglu, Chryssostomos; Ferreri, Carla

doi:10.5772/8774

Cited by 7 publications

(7 citation statements)

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“…[25,26] These radicals, such as superoxide, singlet oxygen, and hydroxyl radicals may be generated as a result of oxygen metabolism during the course of normal cellular activity [26] and may even be helpful in certain settings such as inside a phagocytic vesicle where free radicals may assist in the destruction of invading microorganisms. [27] Elevated levels of free radicals generated during periods of stress due to infection or exposure to toxic compounds may result in damage to host tissues. [25,26] There is, indeed, a significant positive correlation between antioxidative and antitumor activities, suggesting that oxidative mechanisms lay in the basis of proliferation of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

“…Free radicals, continuously derived from either essential metabolic processes or external sources, are associated with the appearance of oxidative stress, which represent a major pathomechanism of numerous diseases, including heart disease, cancer etc [25,26] . These radicals, such as superoxide, singlet oxygen, and hydroxyl radicals may be generated as a result of oxygen metabolism during the course of normal cellular activity [26] and may even be helpful in certain settings such as inside a phagocytic vesicle where free radicals may assist in the destruction of invading microorganisms [27] . Elevated levels of free radicals generated during periods of stress due to infection or exposure to toxic compounds may result in damage to host tissues [25,26] .…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Rašeta

Popović

Knežević

et al. 2020

Chemistry & Biodiversity

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Medicinal mushrooms have tremendous potential in production of bioactive compounds with diverse bioactivities while the biochemical potential of some specific mushroom strains (autochthonous for the region) in production of specific bioactive agents may be of the main importance in a continuous search for novel strains with supreme activities all over the world. In this study, the ethanolic (EtOH) and water (H 2 O) extracts of wild-growing polypore mushroom species were investigated: Trametes versicolor (L.) Lloyd and Stereum subtomentosum Pouzar. This study was designed to determine total phenol (TP), flavonoid (TF) and protein content (TPR) as well as LC/MS/MS phenolic profile related to in vitro antioxidant, antiproliferative (MTT assay) (AP) and DNA fragmentation properties. The H 2 O extracts expressed better antioxidant scavenging potential than EtOH showing the highest activity for the T. versicolor (IC 50 = 5.6 μg/mL, IC 50 = 0.6 μg/mL for DPPH * and OH * , respectively) while O 2 *À activity achieved the best activity for S. subtomentosum (IC 50 = 4.1 μg/mL). In contrary, the highest AP activity was obtained for the EtOH extracts of S. subtomentosum (IC 50 = 141.1 μg/mL). The EtOH extracts of both species showed the highest TP, TF and TPR content. Obtained results of DNA degradation indicate genotoxicity potential of the extracts at high concentration. The LC/MS/MS detection showed that the majority of analyzed extracts contained phenolic acids, p-hydroxybenzoic and protocatechuic acid. The obtained results suggest that analyzed medicinal mushroom species, T. versicolor and S. subtomentosum, could be of potential interest as new sources of strong natural antioxidants as well as antiproliferative agents in the future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Rašeta

Popović

Knežević

et al. 2020

Chemistry & Biodiversity

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“…Initially, the nucleophilic attack of the tertiary amine on the peroxide bond of the BPO molecule followed by a redox reaction generates a benzoyloxy and an anilinomethyl radical (Zoller, Gigmes, & Guillaneuf, ). We presume that the anilinomethyl radical formed during the decomposition of BPO undergoes radical reaction on the sulfur of methionine, which on further desulfurization give the possible product (Chatgilialoglu & Ferreri, ), Therefore, the interaction studies demonstrate the strong binding affinity of the methionine with the residual free radicals in ABC (Figure c).…”

Section: Resultsmentioning

confidence: 99%

Detoxification of poly(methyl methacrylate) bone cement by natural antioxidant intervention

Choppadandi

Kapusetti

2019

J Biomedical Materials Res

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Poly(methyl methacrylate) (PMMA) bone cement is the most widely used grouting material in the joint arthroplasties and vertebroplasties. The present investigation has been carried out to scavenge the radicals and monomer by addition of an antioxidant to minimize the toxicity of bone cement (BC). The in silico studies were employed to determine the potent natural antioxidant at physiological conditions. The antioxidant methionine demonstrated a strong binding affinity with free radicals and methyl methacrylate (MMA) monomer than cysteine. The designated amount of methionine was optimized by various assay methods and >2% methionine shows strong scavenging capacity in BC. Moreover, the antioxidant‐loaded BC (ABC) demonstrated similar handling, physicochemical and mechanical properties to pristine bone cement. Significantly, the developed formulation shows superior biological characteristics such as cell proliferation (2 ± 1 BC and 6 ± 1 ABC), adhesion (0.32 ± 0.02 BC and 0.54 ± 0.01 ABC), and cell viability (81 ± 2% BC and 93 ± 1% ABC) toward human osteoblast‐like cells (MG‐63). Therefore, the novel antioxidant bone cement is a potential candidate for various orthopedic applications to eliminate the adverse effects, related to residual toxic radical and monomer in bone cement.

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“…In our experiments large unilamellar vesicles are obtained by extrusion technique (LUVETs, Fig. 1) with polycarbonate filter of 100 nm diameter, that form an almost transparent suspension, which is also suitable for studies under photolytic conditions [16]. The aqueous and lipid phases are the two distinct compartments of this non-homogeneous system.…”

Section: Methods Of Thiyl Radical Generation: Photochemical Thermal mentioning

confidence: 99%

Reactivity of Thiyl Radicals Generated from Thiomethoxide and Dimethyldisulfide in Microheterogeneous Media

Barata‐Vallejo

Yerien

Postigo

2012

COC

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Sodium thiomethoxide and dimethyldisulfide will be shown to be convenient sources of methyl thiyl radicals for effecting the cis-trans double bond isomerization of oleic acid to elaidic acid residues arranged in phospholipid bilayers of the microheterogeneous aqueous environment of liposomes. The methods used for methyl thiyl radical generation encompass radiolytic and photochemical methods. The convenience of these methyl thiyl radical sources will be interpreted in terms of the turn-over numbers in the radical chain cistrans isomerization reaction of unsaturated lipids.

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Biomimetic Chemistry: Radical Reactions in Vesicle Suspensions

Cited by 7 publications

References 64 publications

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Bioactive Phenolic Compounds of Two Medicinal Mushroom Species Trametes versicolor and Stereum subtomentosum as Antioxidant and Antiproliferative Agents

Detoxification of poly(methyl methacrylate) bone cement by natural antioxidant intervention

Reactivity of Thiyl Radicals Generated from Thiomethoxide and Dimethyldisulfide in Microheterogeneous Media

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