Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds

Zvicer, Jovana; Medic, Ana; Veljović, Djordje; Jevtić, Sanja; Novak, Saša; Obradović, Bojana

doi:10.1016/j.polymertesting.2019.04.004

Cited by 12 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study reported the testing of gellan gum/sol-gel glass composite scaffolds in a perfusion bioreactor under SBF circulation [ 114 ]. The best results in terms of apatite formation on the walls of interior pores were obtained under direct SBF perfusion, while apatite only partially formed on the scaffolds immersed under static mode, with more crystals on the outer surface.…”

Section: The Role Of Testing Mode: Static Vs Dynamic Conditionsmentioning

confidence: 99%

The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges

2020

View full text Add to dashboard Cite

Some special implantable materials are defined as “bioactive” if they can bond to living bone, forming a tight and chemically-stable interface. This property, which is inherent to some glass compositions, or can be induced by applying appropriate surface treatments on otherwise bio-inert metals, can be evaluated in vitro by immersion studies in simulated body fluid (SBF), mimicking the composition of human plasma. As a result, apatite coating may form on the material surface, and the presence of this bone-like “biomimetic skin” is considered predictive of bone-bonding ability in vivo. This review article summarizes the story and evolution of in vitro bioactivity testing methods using SBF, highlighting the influence of testing parameters (e.g., formulation and circulation of the solution) and material-related parameters (e.g., composition, geometry, texture). Suggestions for future methodological refinements are also provided at the end of the paper.

show abstract

Section: The Role Of Testing Mode: Static Vs Dynamic Conditionsmentioning

confidence: 99%

The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges

2020

View full text Add to dashboard Cite

show abstract

“…For instance, biomimetic bioreactors, which imitate physiological conditions in a certain tissue or organ, are primarily being developed for tissue engineering purposes. Still, these bioreactors also provide physiologically relevant studies of novel biomaterials and interactions of biomaterials with cells and tissues [22][23][24]. Thus, by this approach it is possible to address the in vitroin vivo gap that is, discrepancies between results obtained in traditional monolayer cell cultures (in 2D environment) and those found in animal studies [25][26][27].…”

Section: Research In the Field Of Biomaterials Sciencementioning

confidence: 99%

“…Mimicking the physiological conditions is necessary for reliable prediction of biomaterial behavior upon implantation at a certain tissue site. Studies of novel macroporous, composite scaffolds based on gellan gum and bioactive glass particles have shown that the fluid flow in perfusion bioreactors significantly promoted formation of a mineral phase as compared to static conditions, which could be related to scaffold implantation in vascularized tissues [24]. Finally, an integrative approach to optimization of biomaterial properties and operating conditions in biomimetic bioreactors may provide tools for evaluation of new drugs and treatment procedures on 3D models of human tissues, as well as for development of personalized medical therapies, and thus contribute to decrease the level of needed animal experimentation [29].…”

Section: Research In the Field Of Biomaterials Sciencementioning

confidence: 99%

Interdisciplinary crossover for rapid advancements - collaboration between medical and engineering scientists with the focus on Serbia

et al. 2021

Self Cite

View full text Add to dashboard Cite

Over the past decades, development of engineering sciences has incredibly contributed to advancements in medicine by production of numerous devices for diagnostics and treatment. In the middle of the twentieth century, a new scientific field, biomedical engineering (BE), was established, which has developed into an extremely complex scientific discipline requiring a distinctive educational profile. Various study programs in BE have been established at universities around the world but also at several universities in Serbia. Also, intensive research in this field is performed at several scientific institutions in Serbia. In the present paper short summaries of the research results of several groups of engineers and medical doctors are presented as an illustration of the wide field of BE research and possibilities of its application in diagnosis and therapy of various diseases.

show abstract

“…6 Therefore, considerable attention has been given to the development of biomimetic composite scaffolds based on polymers with embedded inorganic calcium phosphate materials. [7][8][9] The most frequently…”

mentioning

confidence: 99%

“…[31][32][33][34] Development of tissue engineering scaffolds, in general, requires comprehensive preclinical characterization that includes physicochemical characterization and cytotoxicity studies, as well as in vivo functionality studies. 9,35 Most studies are focused on investigations of standard physico-chemical properties, such as crystallinity, morphology and chemical composition of scaffold surfaces, porosity and pores size distribution usually at one-time point before and/or after exposure to SBF under static conditions. Scaffold biocompatibility is usually studied in direct contact with cells in monolayers (2D environment), while osteogenic activity and bioactivity of mineral components are evaluated in cultures of cells seeded on scaffolds under static 36 or shaking conditions.…”

mentioning

confidence: 99%

Novel composite scaffolds based on alginate and Mg‐doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions

et al. 2021

Self Cite

View full text Add to dashboard Cite

In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg 2+ were HAP and β-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca 2+ concentration increased while Mg 2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering. K E Y W O R D Sbiomimetic perfusion bioreactor, bone tissue engineering, macroporous scaffolds, β-tricalcium phosphate | INTRODUCTIONBone tissue is reported to be the second most transplanted tissue after blood due to an increasing number of patients with bone defects due to trauma, tumors, infection, and genetic diseases. 1,2 Bone tissue engineering could be a promising strategy to address the shortage of bone transplants by in vitro regeneration of functional tissue equivalents. In recent years, research in this area is focused on developing biomimetic scaffolds with structural similarity to bone tissue and exhibiting bioactive, biocompatible, osteoconductive, and osteoinductive features, as well as suitable mechanical strength, while being cost-effective and easy to handle. [3][4][5] Bone is a composite material comprised of organic components (mostly collagen type I, fibrin, and noncollagenous matrix proteins) reinforced with inorganic calcium phosphate nanostructured crystals. 6 Therefore, considerable attention has been given to the development of biomimetic composite scaffolds based on polymers with embedded inorganic calcium phosphate materials. [7][8][9] The most frequently

show abstract

Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds

Cited by 12 publications

References 37 publications

The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges

The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges

Interdisciplinary crossover for rapid advancements - collaboration between medical and engineering scientists with the focus on Serbia

Novel composite scaffolds based on alginate and Mg‐doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions

Contact Info

Product

Resources

About