Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin

Dou, Leina; Luo, Linpin; Zhang, Xu; Zhang, Wentao; Liu, Lizhi; Yin, Xuechi; Liu, Sijie; Sun, Jing; Zhang, Daohong; Wang, Jianlong

doi:10.1016/j.snb.2020.127956

Cited by 20 publications

(12 citation statements)

References 49 publications

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“…It should be noted that eqn (10) is an approximation because only the first term of the Taylor series in eqn (8) is used. Anyway, eqn (10) is also helpful to explain the near-linear correlations between the light intensity or hue of the colorimetric images and concentration of target analytes in visible regions, 37–45 which are also due to the non-linear response of the CCD in the camera to light intensity (eqn (3)). But the value of G 3 is dependent on the values of G 1 and G 30 .…”

Section: Resultsmentioning

confidence: 99%

A smartphone-based device for simultaneous measurement of ratiometric fluorescence and absorbance demonstrated by the determination of hypochlorous acid

Dou¹,

Shen

Yan

et al. 2022

New J. Chem.

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Section: Resultsmentioning

confidence: 99%

A smartphone-based device for simultaneous measurement of ratiometric fluorescence and absorbance demonstrated by the determination of hypochlorous acid

Dou¹,

Shen

Yan

et al. 2022

New J. Chem.

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“…Bare liposome, 4-NTP@liposome, and Cys@liposome were prepared according to the reported reverse-phase evaporation procedure with minor modification. , In brief, 5 mg of l -α-phosphatidylcholine was dissolved in 1 mL of chloroform. The solvent was completely evaporated to yield a thin lipid film, followed by rehydration with 5 mL of ultrapure water and sonication until the solution became cloudy to obtain bare liposome.…”

Section: Methodsmentioning

confidence: 99%

“…The target DNA-activated trans -cleavage activity of CRISPR/Cas12a was utilized to cleave ssDNA linkers, which was tactfully designed to connect the liposome-amplified platform through the biotin–streptavidin linkage. Within this signal-transducing unit, liposome, possessing spherical lipid bilayer structures with outstanding encapsulation ability, easy surface modification property, and rapid release capability, , was employed to load signal molecules, 4-nitrothiophenol (4-NTP) and cysteine, to achieve dual-mode detection. The dramatically amplified signals were achieved by the triggered breakdown of signal molecule-loaded liposomes.…”

mentioning

confidence: 99%

CRISPR-/Cas12a-Mediated Liposome-Amplified Strategy for the Surface-Enhanced Raman Scattering and Naked-Eye Detection of Nucleic Acid and Application to Food Authenticity Screening

Liu

Chen

et al. 2021

Anal. Chem.

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Surface-enhanced Raman scattering (SERS) has been recognized as a powerful tool for biosensors due to the ultrahigh sensitivity and unique fingerprint information. However, there are some limitations in trace target nucleic acid detection for the restricted signal-transducing and amplification strategies. Inspired by CRISPR/Cas12a with specific target DNA-activated collateral single-strand DNA (ssDNA) cleavage activity and liposome with signal molecule-loading properties, we first proposed a sensitive SERS-based on-site nucleic acid detection strategy mediated by CRISPR/Cas12a with trans-cleavage activity on ssDNA linkers utilized to capture liposomes. Liposomes loading two kinds of signal molecules, 4-nitrothiophenol (4-NTP) and cysteine, could achieve the dual-mode detection of target DNA with SERS and naked eye, respectively. The promptly amplified signals were initiated by the triggered breakdown of signal molecule-loaded liposomes. Emancipated 4-NTP, a biological-silent Raman reporter, would achieve highly selective and sensitive SERS measurement. Released cysteine induced the aggregation of plasmonic gold nanoparticles, leading to an obvious red to blue colorimetric shift to realize portable naked-eye detection. With this strategy, target nucleic acid concentration was dexterously converted into SERS and visualization signals and could be detected as low as 100 aM and 10 pM, respectively. The approach was also successfully applied to determine meat adulteration, achieving the detection of a low adulteration ratio in the complicated food matrix. We anticipate that this strategy will not only be regarded as a universal platform for the on-site detection of food authenticity but also broaden SERS application for the accurate determination of diverse biomarkers.

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“…The constructed surface plasmon resonance (SPR) biosensor recognized E. coli with a detection limit and assay time of 0.57 CFU/ mL and 20 min, respectively. Similarly, in another study by Dou and co-workers, [45] a fluorescence-based biosensor for the recognition of streptolysin O toxin secreted by Streptococcus pyogenes in which a biomimetic liposome membrane was employed to study the specific interaction between streptolysin O and cholesterol. The proposed pathogenic sensor offered a detection limit of 0.33 μg mL À 1 .…”

Section: Biomimetic Receptorsmentioning

confidence: 99%

Principles and Recent Advances in Biosensors for Pathogens Detection

et al. 2021

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Foodborne and waterborne diseases caused by pathogens pose a serious threat to human life, and the detection of such pathogens in food, water, and beverages has gained paramount importance in view of the increasing number of pathogenic diseases in recent times. Standard and traditional analytical techniques employed for the recognition of deadly pathogens, including polymerase chain reaction‐based techniques, immunology‐based assays, culture and colony counting techniques, require several hours or perhaps even a few days for the results. All these techniques, despite being quite sensitive, are time‐intensive. Therefore, several researchers are focusing on the development of rapid pathogen detection techniques. Although the most advanced biosensing technologies exhibit potential approaches, significant research and testing is an urgent need to design innovative biosensors. Novel bioanalytical approaches for the recognition and quantification of target pathogens are being designed and developed to enhance the characteristics of biosensors, including rapid response, selectivity, and sensitivity. In this context, we not only provide an overview of the types of biorecognition elements employed for the detection of pathogens, but also discuss in detail the traditional approaches, biomolecular techniques, the latest advances in the detection and quantification of foodborne and waterborne pathogens, with particular emphasis on biosensors.

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Biomimetic cell model for fluorometric and smartphone colorimetric dual-signal readout detection of bacterial toxin

Cited by 20 publications

References 49 publications

A smartphone-based device for simultaneous measurement of ratiometric fluorescence and absorbance demonstrated by the determination of hypochlorous acid

A smartphone-based device for simultaneous measurement of ratiometric fluorescence and absorbance demonstrated by the determination of hypochlorous acid

CRISPR-/Cas12a-Mediated Liposome-Amplified Strategy for the Surface-Enhanced Raman Scattering and Naked-Eye Detection of Nucleic Acid and Application to Food Authenticity Screening

Principles and Recent Advances in Biosensors for Pathogens Detection

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