Biometric analysis on three offspring of transgenic mutiara catfish (Clarias gariepinus)

Abstract

This research was conducted at the hatchery and the Laboratory of Biotechnology of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran from October to December 2021, with the aim of detecting deformities and phenotypic character abnormalities in G1, G2, and G3 transgenic mutiara catfish (C. gariepinus). The study used a completely randomized design with four treatments (G1, G2, G3 as transgenic mutiara catfish, and non-transgenic mutiara catfish as controls) with three replications. Each treatment used 10 fish (test sample). The results showed the phenotypic characters of body weight (BW), total length (TL), standard length (SL), head length (HL), head width (HW), dorsal length (DL), pectoral length (PCL), and caudal length. (CL) inheritance in G1, G2 and G3 was asymmetric (SD ˃ 1.0). All biometric characters in growth control fish were symmetrical, indicated by SD values < 1.0. Qualitative biometric analysis showed that abnormal fish (body defects) were only found in two fish in G2 and one fish in G3 from a total sample of 10 fish. However, 1 non-transgenic (control) fish was also found. Overall, these defects were only found on the pectoral fins, both in transgenic and non-transgenic catfish, and had little effect on fish growth (still normal).

Growth, Protein Efficiency Ratio and Protein Retention of G6 Transgenic Mutiara Catfish With Mixed Feeding at Different Ratio of Commercial Feed and Rebon Shrimp Flour

“…The results of this research are proven by research by Muhamad (2020) which states that Sangkuriang catfish only has one GH and has a protein retention of 10.8% with feed containing 37.4% protein, which is different from transgenic mutiara catfish which has a protein retention of 42.9%. and in research by Buwono et al (2023) which states that sangkuring fish has a protein retention of 8.49% which has a feed content of 39% while in transgenic mutiara catfish it is 35.8%. If protein retention is below 20%, indicating slow growth like treatment B*, this is the impact of the fish not having exogenous GH gene insertions in non-transgenic fish.…”

“…As a consequence, the absolute growth of transgenic fish is higher than that of non-transgenic fish, both in terms of body weight and absolute length. The results of research conducted by Buwono et al (2015) stated that transgenic mutiara catfish had a growth speed of 2.76 to 3.53 times the size of non-transgenic catfish in G1 and 3 to 4 times in G2 (Buwono et al, 2019).…”

Instant broth powder of mutiara catfish (Clarias gariepinus) using the spray drying method as a natural flavor enhancer

“…The data shown in Table 3 shows that the formula four (F4) broth product complies with SNI standards. The determination of the water content in the instant Mutiara catfish broth powder sample was to find out the limit or maximum range of water content in the sample as the water content affects the purity or product resistance to contaminants (Joesidawati et al 2022;Khalifah et al 2022;Buwono et al 2022). The water content in the sample was 4.6%.…”

“…The essential amino acids in catfish have many benefits for the growth and development of children, for example bone growth and the absorption of calcium. Besides, they are also needed to balance nitrogen in the body and maintain children's body mass so that they are not too fatty (Oonincx and Finke, 2021;Buwono et al 2022;Iswanto et al 2015). "Dumbo" catfish is a type of catfish that is quite commonly consumed and cultivated by fish farmers, it also includes mutiara catfish (Clarias gariepinus) with the advantages of fast growth, size uniformity, disease resistance, salinity and relatively high tolerance to the environment (Jovantheo et al 2022).…”