Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects

Hajji, Sawssen; Khedir, Sameh Ben; Hamza-Mnif, Ibtissem; Hamdi, Marwa; Jedidi, Inès; Kallel, Rim; Boufi, Sami; Nasri, Monçef

doi:10.1016/j.bbagen.2018.10.010

Cited by 114 publications

(44 citation statements)

References 69 publications

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“…The results of XRD are in agreement with other studies which reported that a strong reflection was exhibited around 33°and 40°of the silver nanoparticles (Govindan et al 2012;Hajji et al 2019;Suteewong et al 2018). Therefore, this gives a clear evidence for the preparation of Ch-AgNPs in the current study.…”

Section: Preparation and Characterization Of Ch-agnpssupporting

confidence: 92%

Preparation and antibacterial activity of chitosan-silver nanoparticles for application in preservation of minced meat

2019

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Background: In recent years, the development of efficient and green route to synthesis metal nanoparticles has gained considerable attention in various areas of nanotechnology. Among metal nanoparticles, silver nanoparticles (AgNPs) have attracted much interest because of their potential antimicrobial agents and they are widely applied in many biological and medical fields. With respect to Ag-based nanocomposites, chitosan-silver nanoparticles (Ch-AgNPs) represent an emerging group of bio-nanostructured hybrid materials due to their biocompatibility and biodegradability. Ch is considered a non-toxic biopolymer, as well as its antimicrobial and antifungal activities against a wide range of microorganisms. Methods: Colloidal AgNPs were prepared by chemical reduction of silver ions in the presence of Ch giving Ch-AgNPs. Physiochemical characterizations were determined by scanning electron microscopy (SEM), X-ray diffraction (XRD), and zeta potential. In vitro antibacterial activity was evaluated against Escherichia coli and Salmonella typhimurium using nutrient agar dilution method. The in vivo antibacterial activity was also tested against E. coli in minced meat under aerobic conditions at 4°C for 10 days. In addition, different biochemical parameters were determined in minced meat samples. Results: The colloidal AgNPs formed in situ by chemical reduction of Ag ions in the presence of Ch and showed a good stability. SEM and XRD confirmed the formation of nanoparticles. Zeta potential value was decreased by increase the ratio of Ag and was found to be − 0.225, − 0.193, and − 0.0695 mV for Ag ratio 0.1, 0.2, and 0.35, respectively. The in vitro antibacterial activity of Ch and Ch-AgNPs against E. coli and S. typhimurium reveal that the E. coli was more susceptible to these products than S. typhimurium and Ch-AgNPs have more influence with increasing of the silver concentrations. The in vivo antibacterial activity against E. coli in minced meat samples showed that the effect of Ch-AgNPs was a concentration dependent and greater compared with either controls or Ch alone. Conclusions: The results suggest that Ch-AgNPs could be used in food preservation as antimicrobial agents and for shelf-life extension. However, further toxicological studies on mammals are needed.

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Section: Preparation and Characterization Of Ch-agnpssupporting

confidence: 92%

Preparation and antibacterial activity of chitosan-silver nanoparticles for application in preservation of minced meat

2019

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“…The silver ions interaction takes place through the amino and hydroxyl groups of chitosan. The positively charged amine groups of chitosan a molecule which indicates partial protonation improves the electrostatic interaction with the negative charges of silver nanoparticles [6]. The difference in the zeta potential values might be due to the adsorption of chitosan and silver nanoparticle [21].…”

Section: Uv-vis Absorption Spectra and Zeta Potential Of The Chi-ag-npsmentioning

confidence: 99%

“…The antioxidant activity of Chi-Ag-NPs increased with increase in concentration. The results revealed that the strong effects of synthesized Chi-Ag-NPs may act as natural antioxidants to protect against oxidative stress related with tissue engineering, wound healing and degenerative disease [6].…”

Section: β-Carotene-linoleic Acid Bleaching Model Systemmentioning

confidence: 99%

“…The basic co-polymeric structure of chitosan is the sequence of repeated β (1)(2)(3)(4) linked N-acetyl-d-glucosamine and d-glucosamine units with one amino (NH 2 ) group and two hydroxyl (OH) groups [5]. Chitosan is one of the most extensively exploited biopolymers in the fields of pharmaceutical, tissue engineering and biomedical industries due to its biocompatibility, non-antigenicity, biodegradability, non-toxicity, high permeability, antioxidant and antimicrobial properties [6,7]. Studies by Manukumar et al [1] have shown that bactericidal thymol loaded chitosan silver nanoparticles exerted excellent hemocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi-Ag-NPs): in vitro studies on antioxidant and antibacterial applications

Dara

Mahadevan

Digita³

et al. 2020

SN Appl. Sci.

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Metal-based nanoparticles with potential antioxidant and antimicrobial properties have led to their incorporation of biomaterials for human health care importance. The present investigation is to study on the silver nanoparticles grafted chitosan (Chi-Ag-NPs) composite materials due to their potential applications. Chi-Ag-NPs were synthesized by chemical reduction of silver nitrate salts and biodegradable chitosan. The obtained Chi-Ag-NPs were characterised for Fouriertransform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Transmission electron microscopy (TEM), X-ray powder diffraction, antioxidant and antibacterial properties. UV-visible spectra showed the configuration proficiency of Ag-NPs and chitosan. FTIR spectra indicated that the functional groups of chitosan have particular interactions with Ag-NPs. Particle size and TEM analyses showed that Chi-Ag-NPs were equivalently dispersed in matrix with an average size of 10-230 nm. The surface morphological structures of chitosan has played important role in influencing the adsorption of Ag-NPs. Chi-Ag-NPs were tested for their bioactive properties and results exhibited higher antioxidant and antibacterial activities by the addition of Ag-NPs. Chi-Ag-NPs composites were non-toxic to L929 fibroblast cells. The present investigation suggested that chitosan will be a good source for the preparation of bionanocomposite materials and also could be useful in different industrial and biomedical applications.

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“…In particular, Au nanorods exhibit intense bands of optical absorbance in the so-called therapeutic window and support a photothermal conversion that combines with the physicochemical features of a chitosan or composite hydrogel to bind endogenous tissue or release active ingredients. Other authors have proposed to resort to the biochemical features of Ag nanoparticles in many scaffolds including chitosan/polyvinyl alcohol (PVA) physical hydrogels and electrospun fibers for antimicrobial effects [18][19][20][21][22]. Ag nanospheres probably represent the most successful product of nanomedicine for their antimicrobial properties [11,12,[23][24][25][26][27], which is a critical issue in wound healing.…”

Section: Introductionmentioning

confidence: 99%

Optically activated and interrogated plasmonic hydrogels for applications in wound healing

Milanesi

Magni

Centi

et al. 2020

Journal of Biophotonics

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We disclose the use of hybrid materials featuring Au/Ag core/shell nanorods in porous chitosan/polyvinyl alcohol scaffolds for applications in tissue engineering and wound healing. The combination of Au and Ag in a single construct provides synergistic opportunities for optical activation of functions as near infrared laser tissue bonding, and remote interrogation to return parameters of prognostic relevance in wound healing monitoring. In particular, the bimetallic component ensures optical tunability, enhanced shelf life and photothermal stability, serves as a reservoir of germicidal silver cations, and changes in near‐infrared and visible color according to the environmental level of oxidative stress. At the same time, the polymeric blend is ideal to bind connective tissue upon photothermal activation, and to support fabrication processes that provide high porosity, such as electrospinning, thus putting all the premises for cellular repopulation and antimicrobial protection.

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Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects

Cited by 114 publications

References 69 publications

Preparation and antibacterial activity of chitosan-silver nanoparticles for application in preservation of minced meat

Preparation and antibacterial activity of chitosan-silver nanoparticles for application in preservation of minced meat

Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi-Ag-NPs): in vitro studies on antioxidant and antibacterial applications

Optically activated and interrogated plasmonic hydrogels for applications in wound healing

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