Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi-Ag-NPs): in vitro studies on antioxidant and antibacterial applications

Abstract

Metal-based nanoparticles with potential antioxidant and antimicrobial properties have led to their incorporation of biomaterials for human health care importance. The present investigation is to study on the silver nanoparticles grafted chitosan (Chi-Ag-NPs) composite materials due to their potential applications. Chi-Ag-NPs were synthesized by chemical reduction of silver nitrate salts and biodegradable chitosan. The obtained Chi-Ag-NPs were characterised for Fouriertransform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Transmission electron microscopy (TEM), X-ray powder diffraction, antioxidant and antibacterial properties. UV-visible spectra showed the configuration proficiency of Ag-NPs and chitosan. FTIR spectra indicated that the functional groups of chitosan have particular interactions with Ag-NPs. Particle size and TEM analyses showed that Chi-Ag-NPs were equivalently dispersed in matrix with an average size of 10-230 nm. The surface morphological structures of chitosan has played important role in influencing the adsorption of Ag-NPs. Chi-Ag-NPs were tested for their bioactive properties and results exhibited higher antioxidant and antibacterial activities by the addition of Ag-NPs. Chi-Ag-NPs composites were non-toxic to L929 fibroblast cells. The present investigation suggested that chitosan will be a good source for the preparation of bionanocomposite materials and also could be useful in different industrial and biomedical applications.

Biological Performances of Plasmonic Biohybrids Based on Phyto-Silver/Silver Chloride Nanoparticles

“…Biohybrid Complex I (Ag/AgClNPs–CTS) with the proposed interaction model between the chitosan matrix and silver/silver chloride nanoparticles is shown schematically in Figure 8 . The silver ions’ interaction takes place through the amino and hydroxyl groups of chitosan [ 43 ]. Moreover, it could be assumed that the formation of the chitosan-capped Ag/AgClNPs was confirmed by zeta potential measurements and by SAXS, SEM, and AFM studies.…”

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

“…The spectral bands arising at wavenumbers 1644 and ~1561 cm −1 of CNPs and ACNPs can be assigned to amide I and amide II bands. These amide I and II bands derive from carbonyl (CO) and C–N stretch vibrations 9 . The amide II of ACNPs represents the benzopyran aromatic ring and CC stretching vibrations of anthocyanin 21 .…”

“…Chitosan is a polycationic, biocompatible, non‐toxic, and biodegradable biopolymer. These properties of chitosan make it a versatile candidate for various biomedical and industrial applications, including drug delivery, tissue regeneration, wound healing, semipermeable membranes, purification systems, and as an integral component of nutritional supplements 9 . He et al .…”

Dynamic Mechanical Analysis and Thermal Expansion of Lignin-Based Biopolymers

“…All these three maxims are attributed to the presence of lignin and cellulose in the structure of the composite biopolymer; Another strong band is recorded at 1084 cm −1 , corresponding to deformation of C–O–C anti-symmetrical stretching of polysaccharide [ 46 ] cellulose belonging to this category. Additionally, at 1181 cm −1 , a strong absorption band belonging to the C-O-C ester group is recorded [ 44 ]; Additionally, the presence of lignin and cellulose, respectively, is highlighted by the peaks from 1452 cm −1 and 1382 cm −1 , which are assigned to the asymmetric and symmetric bending deformation vibrations of δasCH3 and δsCH3, [ 43 , 44 , 47 ]. At 1361 cm −1 , a deformation vibration of δOH is visible and [ 44 ] is attributed to the connections made between the biopolymer matrix and the embedded aramid fibers; The absorption band at 1313 −1 indicates the incorporation of the cellulose in to the lignin matrix [ 48 ], and from 1043 cm −1 , according to the scientific literature, coincides with the presence of hemicellulose and pectin [ 11 ]; The low-intensity peaks from 1640 cm −1 (stretching vibration of –C=O, amide I band), 1512 cm −1 and 1542 cm −1 (curved vibration of –N–H) are closely related with the incorporation of aramid fibers into the lignin matrix [ 49 , 50 , 51 , 52 , 53 ]; In the region between 3000 cm −1 and 2850 cm −1 , wavelengths there are a series of four absorption bands (2852 cm −1 , 2875 cm −1 , 2945 cm −1 and 2996 cm −1 ) that are associated with aliphatic C–H stretching [ 43 , 44 , 45 , 54 ] due to the incorporation of natural additives in the Arboform ® LV3 Nature biopolymer.…”

“…All these three maxims are attributed to the presence of lignin and cellulose in the structure of the composite biopolymer; -Another strong band is recorded at 1084 cm −1 , corresponding to deformation of C-O-C anti-symmetrical stretching of polysaccharide [46] cellulose belonging to this category. Additionally, at 1181 cm −1 , a strong absorption band belonging to the C-O-C ester group is recorded [44]; -Additionally, the presence of lignin and cellulose, respectively, is highlighted by the peaks from 1452 cm −1 and 1382 cm −1 , which are assigned to the asymmetric and symmetric bending deformation vibrations of δasCH3 and δsCH3, [43,44,47]. At 1361 cm −1 , a deformation vibration of δOH is visible and [44] is attributed to the connections made between the biopolymer matrix and the embedded aramid fibers; -…”