Background: The optimal internal fixation strategy for vertical femoral neck fractures (VFNFs) in nongeriatric patients remains uncertain. The purpose of this study was to compare the clinical prognoses and underlying mechanical characteristics of the novel off-axis screw technique with dynamic hip screw (DHS) and traditional three parallel screws. Methods: This study included a clinical investigation and a patient-specific finite element analysis (FEA). In the clinical investigation, VFNF patients were grouped by fixation type: (1) three parallel screws (G-TRI); (2) augmentation with an off-axis screw (G-ALP); and (3) DHS with an anti-rotational screw (G-DHS). Fixation failures (non-union, femoral neck shortening (FNS), varus deformation, screw cut-out) and avascular necrosis (AVN) of the three types were compared. In the FEA, twenty-four fixation models with the three fixation types were created based on the data of eight healthy volunteers. Models were assessed under walking conditions. Stiffness, interfragmentary motion (IFM), and implant stress were evaluated. Results: In the clinical investigation, fixation failure rate was significantly (p<0.05) lower in G-ALP (18.5%) than in G-DHS (37.5%) and G-TRI (39.3%). No significant difference of AVN was observed among three fixation groups. In the FEA, stiffness and implant stress in G-DHS models was significantly (p<0.05) higher, and the IFM of G-ALP was significantly (p<0.05) lower among the groups. Conclusions: Among fixation types for VFNFs, the off-axis screw technique exhibited a better interfragmentary stability (lowest IFM), and lower fixation failure rate (especially, FNS). Analyzing interfragmentary stability in biomechanical experiments is more consistent with clinical prognosis than construct stability for VFNFs, suggesting that internal fixations should aim for this outcome.