Biomarkers for the study of catecholamine and serotonin genetic diseases

Ormazábal, Aída; Molero-Luís, Marta; García‐Cazorla, Àngels; Artuch, Rafael

doi:10.1016/b978-0-12-802896-4.00012-2

Cited by 2 publications

(5 citation statements)

References 83 publications

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“…CSF metabolomic analysis is a good analytical tool for the study of the neurometabolic conditions stated here [14]. Furthermore, in such diseases, the quantification of these metabolites in blood/urine is not reliable, because they usually display normal or even paradoxical results [25].…”

Section: Discussionmentioning

confidence: 99%

“…Biogenic amines (5-hydroxyindoleacetic (5-HIAA) and homovanillic (HVA) acids) and pterins (biopterin and neopterin) were analysed as biomarkers of serotonin and dopamine deficiencies (and in the case of neopterin, also as a biomarker of neuroinflammatory conditions) by HPLC with electrochemical and fluorescence detection as previously reported [22]. The vitamins thiamine, thiamine-diphosphate (TDP), 5-methyltetrahydrofolate (5-MTHF) and pyridoxal 5´-phosphate (PLP) were analysed by HPLC with fluorescence detection as reported [14, 22, 24]. Typical chromatograms of these procedures are presented in Additional file 3: Figure S2.…”

Section: Methodsmentioning

confidence: 99%

“…CSF metabolomic investigations have been demonstrated to be a powerful tool for studying specific neurometabolic pathways and related diseases and for exploring metabolic transport from the blood into the brain [14]. Several neurogenetic conditions are caused by specific disturbances in these processes (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…Several neurogenetic conditions are caused by specific disturbances in these processes (Table 1). In recent decades, targeted metabolomic approaches have been used for the study of these neurogenetic conditions [14]. Owing to the important differences in the metabolite concentrations between blood and CSF, contamination of CSF with blood may cause dramatic effects in the measured concentrations of most of the above-mentioned metabolites [15–18].…”

Section: Introductionmentioning

confidence: 99%

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Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins

Batllori

Casado

Sierra

et al. 2019

Fluids Barriers CNS

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BackgroundCerebrospinal fluid (CSF) metabolomic investigations are a powerful tool for studying neurometabolic diseases. We aimed to assess the effect of CSF contamination with blood on the concentrations of selected biomarkers.MethodsCSF samples were spiked in duplicate with increasing volumes of whole blood under two conditions: (A) pooled CSF spiked with fresh whole blood and frozen to cause red blood cell (RBC) lysis; (B) pooled CSF spiked with fresh blood and centrifuged (the supernatant with no RBCs was frozen until the moment of analysis). CSF concentrations of amino acids, biogenic amines, pterins, and vitamins were analysed by HPLC coupled with tandem mass spectrometry, electrochemical and fluorescence detection.ResultsAspartate, glutamate, taurine, ornithine, glycine, citrulline, pyridoxal 5´-phosphate, 5-methyltetrahydrofolate, and thiamine showed higher values when RBCs were lysed when compared with those of CSF with no RBC, while arginine, 5-hydroxyindoleacetic and homovanillic acids showed lower values. When RBCs were removed from CSF, only some amino acids, thiamine and pyridoxal 5´-phosphate showed moderately higher values when compared with the non-spiked CSF sample.ConclusionsCSF-targeted metabolomic analysis is feasible even when substantial RBC contamination of CSF has occurred since CSF centrifugation to remove RBC prior to freezing eliminated most of the interferences observed.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins

Batllori

Casado

Sierra

et al. 2019

Fluids Barriers CNS

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“…Kennedy et al observed an excellent correlation between salivary catecholamine metabolite levels and plasma metabolite levels (r = 0.63) and noted that metabolite-to-catecholamine ratios were higher in saliva than in blood-a feature attributed to more rapid penetration of these metabolites into saliva from the bloodstream. One disadvantage of using these compounds as biomarkers is their low abundance and low stability due to rapid decomposition (Ormazabal et al, 2017). Catecholamines are metabolized with dopamine being converted into homovanillic acid (HVA), noradrenaline into normetanephrine, vanillylmandelic acid (VMA), and 3methoxy-4-hydroxyphenylglycol (MHPG), and adrenaline into metanephrine, VMA, and MHPG.…”

Section: Catecholamines and Metabolitesmentioning

confidence: 99%

Biomarkers and biosensors for the diagnosis of noncompliant pH, dark cutting beef predisposition, and welfare in cattle

Nelis

Bose

Broadbent

et al. 2022

Comp Rev Food Sci Food Safe

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Meat quality can be affected by stress, exhaustion, feed composition, and other physical and environmental conditions. These stressors can alter the pH in postmortem muscle, leading to high pH and low‐quality dark cutting (DC) beef, resulting in considerable economic loss. Moreover, the dark cutting prediction may equally provide a measure for animal welfare since it is directly related to animal stress. There are two needs to advance on‐site detection of dark cutters: (1) a clear indication that biomarker (signature compounds) levels in cattle correlate with stress and DC outcome; and (2) measuring these biomarkers rapidly and accurately on‐farm or the abattoir, depending on the objectives. This critical review assesses which small molecules and proteins have been identified as potential biomarkers of stress and dark cutting in cattle. We discuss the potential of promising small molecule biomarkers, including catecholamine/cortisol metabolites, lactate, succinate, inosine, glucose, and β‐hydroxybutyrate, and we identify a clear research gap for proteomic biomarker discovery in live cattle. We also explore the potential of chemical‐sensing and biosensing technologies, including direct electrochemical detection improved through nanotechnology (e.g., carbon and gold nanostructures), surface‐enhanced Raman spectroscopy in combination with chemometrics, and commercial hand‐held devices for small molecule detection. No current strategy exists to rapidly detect predictive meat quality biomarkers due to the need to further validate biomarkers and the fact that different biosensor types are needed to optimally detect different molecules. Nonetheless, several biomarker/biosensor combinations reported herein show excellent potential to enable the measurement of DC potential in live cattle.

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Biomarkers for the study of catecholamine and serotonin genetic diseases

Cited by 2 publications

References 83 publications

Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins

Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins

Biomarkers and biosensors for the diagnosis of noncompliant pH, dark cutting beef predisposition, and welfare in cattle

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