Biomarkers for patients with interstitial cystitis/bladder pain syndrome

Liu, Hsin-Tzu; Kuo, Hann‐Chorng

doi:10.1016/j.urols.2015.02.002

Cited by 14 publications

(16 citation statements)

References 57 publications

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“…Studies investigating the expression of these 4 cytokines in humans with IC/PBS have not been reported. Although we did not detect significant differences in other serum cytokines in FIC cats compared to controls, increased serum or plasma concentrations of IL‐1β, IL‐6, CXCL8 (IL‐8), and TNF‐α have been reported in people with IC/PBS compared to healthy controls . Factors responsible for differences in cytokine profiles between acute FIC and IC/PBS are unknown, but may be related to differences in chronicity of disease, pathophysiologic mechanisms, and analytical performance of cytokine assays.…”

Section: Discussioncontrasting

confidence: 59%

Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Parys

Yuzbasiyan‐Gurkan

Kruger

2018

Veterinary Internal Medicne

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BackgroundFeline idiopathic cystitis (FIC) is a common lower urinary tract disorder of domestic cats that resembles interstitial cystitis/painful bladder syndrome (IC/PBS) in humans. Diagnosis of FIC is based on clinical signs and exclusion of other disorders because of a lack of specific pathologic findings or other objective biomarkers. Cytokines are potential noninvasive biomarkers to define the presence, severity, and progression of disease, and response to treatment.ObjectivesThe objective of this pilot study was to determine concentrations of selected cytokines in serum from healthy cats and cats with acute FIC.AnimalsSerum samples from 13 healthy cats and from 12 cats with nonobstructive acute FIC were utilized.MethodsMultiplex analysis of 19 cytokines (CCL2, CCL5, CXCL1, CXCL12, CXCL8, Flt3L, GM‐CSF, IFN‐γ, IL‐12 (p40), IL‐13, IL‐18, IL‐1β, IL‐2, IL‐4, IL‐6, PDGF‐BB, SCF, sFas, and TNF‐α) was performed with a commercially available feline‐specific multiplex bead‐based assay.ResultsMean serum concentrations of IL‐12 (p40; P < 0.0001), CXCL12 (P = 0.002), IL‐18 (P = 0.032), and Flt3L (P = 0.0024) were significantly increased in FIC cats compared to healthy cats. GM‐CSF, IL‐1b, IL‐2, and PDGF‐BB were undetectable or detected in an insufficient number of cats to allow meaningful comparisons.Conclusions and Clinical ImportanceWe have identified increased serum concentrations of pro‐inflammatory cytokines and chemokines CXCL12, IL‐12, IL‐18, and Flt3L in FIC‐affected cats. These findings suggest potential candidates for noninvasive biomarkers for diagnosis, staging, and therapeutic outcome monitoring of affected cats and provide additional insight into the etiopathogenesis of FIC.

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Section: Discussioncontrasting

confidence: 59%

Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Parys

Yuzbasiyan‐Gurkan

Kruger

2018

Veterinary Internal Medicne

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“…Due to the penetration of urine through the affected areas, interstitial tissues were irritated resulting in a decrease in the protective properties of the mucous membrane. Chronic inflammation leads to fibrosis of the bladder wall and decrease in the bladder capacity-as a result, wrinkled tissues do not function at full strength, their accumulative and excretory functions are impaired eventually (3,16). Our results are comparable with data from the study by Steers and Tuttle (14).…”

Section: Discussionsupporting

confidence: 87%

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

Sholan¹

2020

Haseki

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The aim of this study was to determine the level of nerve growth factor (NGF) and epidermal growth factor (EGF) in an animal model of interstitial cystitis/bladder pain syndrome (IC/ BPS) and identify correlations between them. Methods: IC/BPS modeling was performed on white New Zealand female rabbits divided into six groups depending on the methods of modeling. Urine and blood levels of NGF and EGF were determined by the ELISA method. The correlation between the indicators was calculated by the Pearson correlation coefficient. Results: An increase in the concentration of NGF and EGF was detected in animals of all experimental groups; a significantly high level of these factors in blood and urine was determined in a toxic model created with the introduction of urine into the submucosal layer of the bladder wall. The correlation between NGF and EGF tended to increase by 14 days. There was a strong correlation between these indicators in the urine of animals with IC/BPS created by a 70% alcohol solution (p<0.01). A strong correlation between these indicators was observed in blood of animals with IC/BPS created by the administration of protamine sulfate and in animals of toxic model (p<0.01). Conclusion: An increase in the levels of proliferation markers is likely due to chronic inflammation process and urine toxicity. The presence of a correlation between NGF and EGF and their strengthening as the disease progresses indicates an increase in the proliferation processes. Proliferation markers can be used in the diagnosis and monitoring of IC/BPS.

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“…Наши результаты согласуются с данными других исследований [17]. В ряде сообщений указано на повышение уровня ФРН в тканях мочевого пузыря в экспериментальных моделях ИЦ у крыс [9, [17][18][19]. Ряд исследователей считает, что воспаление повышает экспрессию ФРН и это имеет существенное патогенетическое значение при развитии ИЦ/СБМП [9,19].…”

Section: Discussionunclassified

“…Клинические и экспериментальные данные указывают на повышение уровня ФРН в ткани мочевого пузыря и моче пациентов с ИЦ/СБМП [5,9,18]. Мы также обнаружили повышенный уровень ФРН в моче у животных с различными моделями ИЦ/СБМП, но статистически значимо высокий уровень ФРН отмечался лишь в варианте с введением мочи в стенку мочевого пузыря.…”

Section: Discussionunclassified

The level of nerve growth factor and its relationship with the content of leukocytes and mast cells in experimental interstitial cystitis/painful bladder syndrome

Sholan

оглы²

2019

Urolog vedom

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Relevance. Interstitial cystitis/painful bladder syndrome (IC/PBS) is a debilitating condition of pain and discomfort in the bladder. Due to the limited number of published studies in this area, there is a need for further research. Objective: to evaluate the level of nerve growth factor and its relationship with various types of leukocytes and mast cells of the bladder tissue in animals with experimental models of interstitial cystitis/painful bladder syndrome. Material and methods. IC/PBS modeling was performed on 38 female rabbits. The IC/PBS modeling was created by introducing HCl into the bladder (group 1), urine into the wall of the bladder (group 2), and physiological saline (group 3). Nerve growth factor (NGF) was determined by the ELISA method, and white blood cells along with mast cells in tissues were determined by histological processing. Results. A statistically significant high level of NGF in blood and urine was observed on the 1st day after IC/PBS initiation in groups 13 and in group 2 respectively. Two weeks after the initiation of IC/PBS in animals of group 1 a decrease in the level of FRN in the blood by 29.3% and its increase in urine by 14.3% was observed; in group 2 an increase by 65.5% (p 0.01) in blood and by 52.7% (p 0.01) in urine was observed and in group 3 a decrease by 30.8% (p 0.05) in blood and by 30.5% (p 0.05) in urine was observed. The greatest number of leukocytes was determined in the tissue of the bladder in animals of the 1st group. Mast cell infiltration was observed in groups 1 and 2. Correlation between the NGF in blood and urine and between NGF and the number of leukocytes and mast cells was revealed. Findings. In IC/PBS, the level of NGF in the blood and urine is increased. Indicators of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs when damage to the integrity of the bladder by urinary toxicity. Nerve growth factor correlates with leukocyte and mast cell infiltration in the bladder tissue. Conclusion. During IC/PBS, the level of NGF in the blood and urine is increased. Levels of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs as a response to the damage of the bladder wall integrity caused by urinary toxicity. The level of nerve growth factor correlates with the bladder tissue intensity of infiltration with leukocytes and mast cells.

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Biomarkers for patients with interstitial cystitis/bladder pain syndrome

Cited by 14 publications

References 57 publications

Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Evaluation of the Proliferation Markers in Interstitial Cystitis/Bladder Pain Syndrome: An Experimental Model

The level of nerve growth factor and its relationship with the content of leukocytes and mast cells in experimental interstitial cystitis/painful bladder syndrome

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