Biomarker of pyrethrum exposure

Leng, G.; Gries, Wolfgang; Selim, Sami

doi:10.1016/j.toxlet.2005.09.030

Cited by 48 publications

(24 citation statements)

References 18 publications

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“…The main metabolite of permethrin employed as marker of permethrin intake through food is the 3-phenoxybenzoic acid (PBA). As reported in literature, the range of PBA in human urine is wide, and it can be included between 0.0012 lM and 23 lM/L urine/24 h [9][10][11]. Hence, in our study, we chose the levels of permethrin to be in the range of 5-20 lM, which was sufficient to induce damage to the heart cells.…”

Section: Discussionmentioning

confidence: 99%

“…This value corresponds to 4-9 lM/day of permethrin. To identify the environmental pesticide level, some authors monitorize the urinary excretion of its metabolites [9][10][11]. The main metabolite of permethrin is 3-phenoxybenzoic acid (PBA) which ranges from 0.0012 to 23 lM/L urine/ 24 h [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…To identify the environmental pesticide level, some authors monitorize the urinary excretion of its metabolites [9][10][11]. The main metabolite of permethrin is 3-phenoxybenzoic acid (PBA) which ranges from 0.0012 to 23 lM/L urine/ 24 h [8][9][10]. If we consider that the volume of urine excreted daily by human is about 1.8 L/day, the amount of PBA excreted daily is found within a range of 0.00216-41.4 lM [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

2010

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Pollutants including insecticides have been recently reported to be a risk factor involved in various diseases. Permethrin, a member of the family of synthetic pyrethroids, is widely used as insecticide in agriculture and other domestic applications. To investigate possible cardiotoxicity, we had examined different concentrations of permethrin on the freshly isolated rat heart cells using the alkaline comet assay. A significant difference in % tail DNA between all concentrations of permethrin (5, 10, 20 microM) and vehicle (control) without enzymes and with Fpg-treated cells were measured. The results indicated that permethrin induced oxidative damage to purine bases in the heart cells. Pyrimidines oxidation was evaluated using Endonuclease III (Endo III), but the results did not reveal any significant changes. After permethrin exposure, cells were studied to evaluate their DNA repair capacity. A complete DNA repair at 10 and 20 microM was measured after 30 and 60 min of repair intervals. Significant change in plasma membrane fluidity at different depths of bilayer was measured following permethrin treatment. Membrane fluidity in the hydrophilic-hydrophobic region was reduced, while the hydrophobic inner resulted more fluid following permethrin treatment of heart cells. This work points to standardize conditions applicable to ex vivo cells following in vivo treatment in order to study the cardiotoxicity of insecticide.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

2010

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“…Several methods exist for the measurement of synthetic pyrethroid metabolites in human urine. (Leng et al, 1997;Aprea et al 1997;Maroni et al, 2000c;Smith et al, 2002;Schettgen et al, 2002;Leng et al, 2006;Fortin et al,2008). The metabolites 3-…”

Section: Pyrethroids Metabolitesmentioning

confidence: 99%

Biological Markers of Human Exposure to Pesticides

Araoud¹

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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“…Urine samples were hydrolyzed and derivatized using 1,1,1,3,3,3-hexafluoroisopropanol and N, N-diisopropylcarbodiimide; the concentration of 3-PBA was then quantified by gas chromatography-mass spectrometry equipped with the electroionization system (Agilent 5975 MDS system; Agilent, Santa Clara CA) using 8 lg/ml of 2-PBA as an internal standard. The analytical method used in this study has been detailed elsewhere [30]. Urine specimens collected and pooled from healthy volunteers were used for quality control.…”

Section: Determination Of Urinary Metabolitesmentioning

confidence: 99%

Relationship between dietary habits and urinary concentrations of 3-phenoxybonzoic acid in a middle-aged and elderly general population in Japan

Kimata

Kondo

Ueyama

et al. 2009

Environ Health Prev Med

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Objectives The ingestion of pesticides in the daily diet is assumed to be the main modality of pesticide exposure for most people. A widely used class of pesticides in agricultural or residential settings is pyrethroid. We have examined the relationship between the intake frequency of selected items of vegetables and fruits and urinary metabolites of pyrethroid pesticides in a healthy general population. Methods A total of 535 residents (184 men and 351 women) who attended a healthcare checkup program conducted in a rural area of Hokkaido, Japan, in August 2005 provided informed consent for their spot urine samples to be used for the determination of 3-phenoxybenzoic acid (3-PBA) levels. They also completed a self-administered questionnaire regarding the intake frequency of 12 food items. The concentrations of creatinine-corrected 3-PBA were predicted by the intake frequency of each item, using analysis-of-covariance models to adjust for age, sex, body mass index, and drinking and smoking status. Results Both a significant association between the 3-PBA concentration and the frequency of tomato consumption and a significant positive linear trend was found in female subjects. In contrast, no such association was found in the male subjects. Conclusions The frequency of tomato consumption was confirmed to strongly predict the urinary pyrethroid metabolite levels in the general population-presumably because tomatoes are most often consumed raw and unpeeled (more so than all other vegetables and fruits analyzed in the current study). However, it should be noted that the 3-PBA levels, even among those subjects with the highest consumption of tomatoes, were far below the levels of toxicological significance, although the health consequences from long-term low-level exposure to pyrethroid requires further exploration.

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Biomarker of pyrethrum exposure

Cited by 48 publications

References 18 publications

Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

Purine Bases Oxidation and Repair Following Permethrin Insecticide Treatment in Rat Heart Cells

Biological Markers of Human Exposure to Pesticides

Relationship between dietary habits and urinary concentrations of 3-phenoxybonzoic acid in a middle-aged and elderly general population in Japan

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