Biomarker induction in tropical fish species on the Northwest Shelf of Australia by produced formation water

Zhu, Shiqian; King, Sue; Haasch, Mary L.

doi:10.1016/j.marenvres.2007.11.007

Cited by 27 publications

(9 citation statements)

References 37 publications

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“…Biomarker analyses have also shown that fish held in cages close to the PW discharge outlet show a small but detectable induction of Vtg and CYP1A (King et al, 2005;Scott et al, 2006a;Zhu et al, 2008). In addition, a size dependent increase in the levels of Vtg in plasma has been found in wild male cod from the North Sea (Scott et al, 2006b).…”

Section: Environmental Implications Of Our Results Compared To the Simentioning

confidence: 98%

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

Meier

Morton

Nyhammer

et al. 2010

Marine Environmental Research

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Produced water (PW) contains numerous toxic compounds of natural origin, such as dispersed oil, metals, alkylphenols (APs), and polycyclic aromatic hydrocarbons (PAHs). In addition, PW also contains many different chemicals which have been added during the oil production process. In the study described here, cod were exposed to real PW collected from an oil production platform in the North Sea. This was done in order to best recreate the most realistic field-exposure regime in which fish will be affected by a wide range of chemicals. The biological effects found in this study therefore cannot be assigned to one group of chemicals alone, but are the result of exposure to the complex chemical mixture found in real PW. Since APs are well known to cause endocrine disruption in marine organisms, we focused our chemical analysis on APs in an attempt to better understand the long-term effects of APs from PW on the biology of fish. In this study, cod were exposed to several concentrations of real PW and 17β-oestradiol (E(2)), a natural oestrogen, at different developmental stages. Cod were exposed to PW either during the embryo and early larvae stage (up to 3 months of age) or during the early juvenile stage (from 3 to 6 months of age). Results showed that, in general, APs bioconcentrate in fish tissue in a dose and developmental stage dependent manner during PW exposure. However, juveniles appeared able to effectively metabolise the short chain APs. Importantly, PW exposure had no effect on embryo survival or hatching success. However, 1% PW clearly interfered with the development of normal larval pigmentation. After hatching most of the larvae exposed to 1% PW failed to begin feeding and died of starvation. This inability to feed may be linked to the increased incidence of jaw deformities seen in these larvae. In addition, cod exposed to 1% PW, had significantly higher levels of the biomarkers vitellogenin and CYP1A in plasma and liver, respectively. No similar effects were seen in cod exposed to either 0.1% or 0.01% PW.

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Section: Environmental Implications Of Our Results Compared To the Simentioning

confidence: 98%

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

Meier

Morton

Nyhammer

et al. 2010

Marine Environmental Research

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“…exposed trout to 5 % WAF, 96 h, and exposed wild trout) appears to down-regulate CYP2K1. More studies are needed to understand if down-regulation of liver CYP2K1 and CYP2M1 and gill CYP2K1 were caused by the disruption of physiologic homeostatic processes in which CYP2K1 and CYP2M1 enzymes participate (Zhu et al 2008, Browne et al 2010, or by alterations in the metabolism of estrogenic hormones (testosterone, progesterone, estradiol) and fatty acids, which could lead to the inhibition of CYP2K1 and CYP2M1 transcript expression, for example, by estradiol , Sovadinová 2006. Although the results in the WAF experiment are not conclusive, differences among time response of CYP2 expression could be useful as complementary indicators of pollution by crude oil.…”

Section: Discussionmentioning

confidence: 99%

“…CYP2K1 has benzphetamine N-demethylase and steroid hydroxylase activities, and both CYP2K1 and CYP2M1 are involved in lauric acid metabolism and some longer fatty acids in rainbow trout (Yang et al 1998 and both are involved in the metabolism of estrogenic hormones Wang-Buhler 1998, Buhler et al 2000). A tropical reef fish exposed to oily water showed an induction of CYP2K1 and CYP2M1 proteins (Zhu et al 2008). Thus, anomalous expression of both CYP2 could impact on steroid and fatty acid homeostasis in rainbow trout (e.g.…”

Section: Introductionmentioning

confidence: 99%

CYPS MOLECULAR BIOMARKERS IN RAINBOW TROUT Oncorhynchus mykiss TO ASSESS OIL CONTAMINATION IN A PATAGONIAN STREAM

Leggieri

Anna²,

Cerón³

et al. 2017

Rev. Int. Contam. Ambie.

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Cytochrome P450 (CYP) gene expressions were studied by semi-quantitative polymerase chain reaction (PCR) to assess oil pollution using liver and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream (Bariloche, Argentina), up and downstream of a discharge of petroleum spill from an unproductive wellbore. Data was complemented with laboratory exposure to water accommodated fraction (WAF) from the oil spill. Oil exposure induced gill and liver CYP1A transcription (1.2-1.4-fold), which was used as reference biomarker. In both organs, CYP2K1 transcript was induced after 5 % WAF exposure, 24 h (1.8-2.0-fold), and it was down-regulated in more extended time (1.2-1.5-fold). CYP2M1 transcript levels were down-regulated in liver of impacted wild trout and in trout exposed to 5 % WAF during 96 h (1.5-1.7-fold). CYP2K1 and CYP2M1 are involved in the metabolism of fatty acids and estrogenic hormones, and its down-regulation would indicate metabolic alterations. CYP3A27 levels were only induced in liver of wild trout (1.6-fold), possibly related to crosstalk between aryl hydrocarbon receptor and pregnane X receptor signaling pathway. While CYP1A mRNA expression appears to be a more robust biomarker for detecting the effects of crude oil under different dose and exposure time, the expression of CYP2K1, CYP2M1 and CYP3A27 mRNAs would indicate different time exposure to oil pollution, particularly under high oil concentration in water. Hence, patterns of multiple CYP genes expression in rainbow trout gills and liver could be especially valuable for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems. L.R. Leggieri et al. 682Palabras clave: CYP450, derrame de petróleo, peces, arroyo contaminado, Patagonia RESUMEN Se estudió la expresión génica de citocromos P450 (CYP) mediante reacción semicuantitativa en cadena de la polimerasa (PCR, por sus siglas en inglés), en muestras de hígado y branquias de trucha arcoíris Oncorhynchus mykiss juveniles capturadas en un arroyo que es afectado por un derrame de petróleo. El estudio se completó con exposiciones in vivo a la fracción soluble del petróleo del vertedero (WAF). El CYP1A, usado como biomarcador de referencia, fue inducido por el petróleo (1.2-1.4-veces) en branquias e hígado. En ambos órganos, el CYP2K1 fue inducido después de 24 h con 5 % WAF (1.8-2.0-veces), y subregulado en tiempos más extensos (1.2-1.5-veces). CYP2M1 fue subregulado en el hígado de truchas contaminadas y de aquellas expuestas a 96 h con 5 % (1.5-1.7-veces). Dado que CYP2K1 y CYP2M1 participan del metabolismo de ácidos grasos y de hormonas estrogénicas, la subregulación indicaría alteraciones en estos metabolismos. CYP3A27 fue inducido sólo en el hígado de las truchas silvestres afectadas por petróleo, posiblemente por efectos cruzados entre las señalizaciones de los receptores de hidrocarburos de arilo y X de pregnano. Mientras que la expresión de CYP1A mRNA pareciera ser un biomarcador más robusto para detectar los efectos d...

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“…Due to the toxicity of the PAH metabolites the oil industry in some areas of the world (e.g. North Sea, Mediterranean Sea, Australian Northwest Shelf, Gulf of Mexico) are required to monitor their discharges to the aquatic environment [46][47][48][49].In the North Sea this is taken care of through the Water Column Monitorin programs [48]. For monitoring purposes the 16 EPA compounds has been chosen as the most important PAHs to analyze for.…”

Section: Oil As a Source Of Polycyclic Aromatic Hydrocarbons To The Amentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Pampanin¹,

Sydnes²

2013

Hydrocarbon

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Biomarker induction in tropical fish species on the Northwest Shelf of Australia by produced formation water

Cited by 27 publications

References 37 publications

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

CYPS MOLECULAR BIOMARKERS IN RAINBOW TROUT Oncorhynchus mykiss TO ASSESS OIL CONTAMINATION IN A PATAGONIAN STREAM

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

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