Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds

Kudryasheva, Nadezhda S.; Kovel, Ekaterina S.; Sachkova, Anna S.; Vorobeva, Anna A.; Isakova, Viktoriya G.; Чурилов, Г. Н.

doi:10.1111/php.12639

Cited by 21 publications

(28 citation statements)

References 37 publications

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“…Changes in general toxicity and oxidative toxicity of aqueous media under exposure to humic substances were studied previously in [47][48][49]. Later [41,[50][51][52], the toxicity and antioxidant activity of a series of fullerenols (i.e., carbon nanostructures, water-soluble derivatives of fullerenes, perspective pharmaceutical agents) were evaluated and compared. Effects of MNPs and MNPs with modified surface on bacterial enzyme reactions have not been studied yet.…”

Section: Introductionmentioning

confidence: 99%

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

et al. 2020

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Current paper presents biological effects of magnetite nanoparticles (MNPs). “Relations of MNP’ characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus.”. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc.

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Section: Introductionmentioning

confidence: 99%

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

et al. 2020

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“…Decrease of both general and oxidative toxicities under addition of humic substances (products of natural decomposition of organic matter in soil and bottom sediments) was studied in [37] , [38] , [39] . Description of the enzyme bioluminescent technique to evaluate antioxidant activity and toxicity of bioactive compounds is presented in [40] . It should be noted that, similar to reactive oxygen species, active chlorine species can also produce toxic effects [41] .…”

Section: Introductionmentioning

confidence: 99%

On mechanism of antioxidant effect of fullerenols

Sachkova

Kovel

Чурилов

et al. 2017

Biochemistry and Biophysics Reports

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Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10−5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10−8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.

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“…Figure 11. Antioxidant activity of humic substances was studied in [80][81][82][83][84] and fullerenols -in [84][85][86][87]. Cellular and enzymatic assays were used to evaluate a General Toxicity (GT) under conditions of the oxidative stress; this stress was modeled in the solutions of exogenous organic or inorganic oxidizers (1,4-benzoquinone or potassium ferricianide, respectively).…”

Section: Luminescence Bioassays As Tools For Study Low-dose Radiationmentioning

confidence: 99%

“…The latter appears in the presence of Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 21 August 2019 doi:10.20944/preprints201908.0221.v1exogenous oxidizers, depending on their redox potential and concentration[25]. Justification for GTand OxT application to evaluate toxicity of bioactive compounds is presented in[80][81][82][83][84][85][86][87].Principle of antioxidant efficiency evaluation is presented inFigure forbacteria-based (upper line) and enzyme-based (lower line) bioassays; humic substances are chosen here as an example of an antioxidant agent.…”

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Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

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Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

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Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds

Cited by 21 publications

References 37 publications

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

On mechanism of antioxidant effect of fullerenols

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

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