Bioluminescence screening for bacteriuria

Kolbeck, J C; Padgett, R A; Estevez, E G; Harrell, Lizzie J.

doi:10.1128/jcm.21.4.527-530.1985

Cited by 19 publications

(7 citation statements)

References 13 publications

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“…The present data, together with other data (2), suggest the necessity to determine a RLU interval to distinguish infected from noninfected urine specimens, instead of using a single breakpoint value, as suggested by other authors (7,9,11,12).…”

Section: Discussionsupporting

confidence: 72%

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Reliability of a bioluminescence ATP assay for detection of bacteria

et al. 1992

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The reliability of bioluminescence assays which employ the luciferin-luciferase ATP-dependent reaction to evaluate bacterial counts was studied, both in vitro and on urine specimens. Bioluminescence and cultural results for the most common urinary tract pathogens were analyzed. Furthermore, the influence of the culture medium, of the assaying method, and of the phase of growth on bioluminescence readings was studied. Results show that Proteus, Providencia, and Morganella strains are not correctly detected, neither in vitro nor in urine samples, by the standard assaying method. The analysis of assaying parameters demonstrated that some modifications to the extraction procedure of bacterial ATP could improve the reliability of this technique. Rapid methods to evaluate bacterial counts in biological specimens have been continuously investigated in the last 20 years (1, 4, 6, 14). The calibrated loop or the serial dilution technique is used in most cases, though they are timeconsuming (7). Methods employing the measurement of bacterial ATP by the luciferin-luciferase, ATP-dependent reaction have been developed and marketed. They were shown to be sufficiently sensitive to detect bacteria in urine specimens containing 105 CFU/ml (2, 7, 9, 11, 12). Studies performed on clinical specimens suggested different relative light unit (RLU) values (ranging from 146 to 700) as cutoff values to separate infected from noninfected urine samples. Some authors (1, 4, 7) reported 2 to 4% false-negative tests among specimens infected by Proteus mirabilis or mixed flora, but these authors did not investigate the causes of such false-negative results or evaluate the correlation between CFU-per-milliliter and RLU values on pure cultures of different bacterial species. This paper presents the results of an investigation of the causes of false-negative bioluminescence assays and the correlation between CFU-per-milliliter and RLU values for the most common urinary tract pathogens.

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Section: Discussionsupporting

confidence: 72%

“…The measurement of bacterial ATP by the luciferin-luciferase reaction is one of the most interesting (5,8,15). Some authors reported a certain incidence of false negatives when tests were performed on specimens containing mixed flora or P. mirabilis (7,10).…”

Section: Discussionmentioning

confidence: 99%

Reliability of a bioluminescence ATP assay for detection of bacteria

et al. 1992

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“…Bioluminescent bacteria are prokaryotes that can produce their own light solely from their unique metabolic activity. Marine luminous bacteria have been studied and extensively utilized in the biotechnological detection of toxic chemicals, pollutants, pesticides, dissolved oxygen, freshness, quorum sensing, ATP, and other physicochemical conditions [17][18][19][20][21][22][23]. Bioluminescence is living light; the partial or complete inhibition depends on the cells' viability, which is affected by the cells' immediate physicochemical condition and/or direct exposure to toxicants.…”

Section: Introductionmentioning

confidence: 99%

An Intact Cell Bioluminescence-Based Assay for the Simple and Rapid Diagnosis of Urinary Tract Infection

Reyes

Fuentes

et al. 2020

IJMS

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Urinary tract infection (UTI) is one of the most common infections, accounting for a substantial portion of outpatient hospital and clinic visits. Standard diagnosis of UTI by culture and sensitivity can take at least 48 h, and improper diagnosis can lead to an increase in antibiotic resistance following therapy. To address these shortcomings, rapid bioluminescence assays were developed and evaluated for the detection of UTI using intact, viable cells of Photobacterium mandapamensis USTCMS 1132 or previously lyophilized cells of Photobacterium leiognathi ATCC 33981™. Two platform technologies—tube bioluminescence extinction technology urine (TuBETUr) and cellphone-based UTI bioluminescence extinction technology (CUBET)—were developed and standardized using artificial urine to detect four commonly isolated UTI pathogens—namely, Escherichia coli, Proteus mirabilis, Staphylococcus aureus, and Candida albicans. Besides detection, these assays could also provide information regarding pathogen concentration/level, helping guide treatment decisions. These technologies were able to detect microbes associated with UTI at less than 105 CFU/mL, which is usually the lower cut-off limit for a positive UTI diagnosis. Among the 29 positive UTI samples yielding 105–106 CFU/mL pathogen concentrations, a total of 29 urine specimens were correctly detected by TuBETUr as UTI-positive based on an 1119 s detection window. Similarly, the rapid CUBET method was able to discriminate UTIs from normal samples with high confidence (p ≤ 0.0001), using single-pot conditions and cell phone-based monitoring. These technologies could potentially address the need for point-of-care UTI detection while reducing the possibility of antibiotic resistance associated with misdiagnosed cases of urinary tract infections, especially in low-resource environments.

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“…Quantitative urine culture is considered the standard procedure for adequate diagnosis of urinary-tract infections (25). Urine cultures represent 40 to 70% of the specimens sent for examination to clinical-microbiology laboratories (7,14). Although the prevalence of urinary infections may vary in different patient populations, approximately 80% of urine cultures are negative (7,14,33).…”

mentioning

confidence: 99%

“…Urine cultures represent 40 to 70% of the specimens sent for examination to clinical-microbiology laboratories (7,14). Although the prevalence of urinary infections may vary in different patient populations, approximately 80% of urine cultures are negative (7,14,33). In an attempt to reduce the cost and time expended in examining these negative cultures, several rapid methods have been developed for characterizing bacteriuria, including microscopic examination, chemical tests, and automated systems (6,7,17).…”

mentioning

confidence: 99%

Simplified Technique for Detection of Significant Bacteriuria by Microscopic Examination of Urine

Cardoso¹,

Muraro

Siqueira³

et al. 1998

J Clin Microbiol

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A comparative study of microscopic examination of 10 μl (simplified loop technique) and 50 μl (traditional drop technique) of uncentrifuged Gram-stained urine specimens for detection of significant bacteriuria was carried out. The results demonstrated that the 10-μl loop technique can be used as an alternative to the 50-μl drop technique for presumptive diagnosis of urinary-tract infection in bacteriological practice, with the advantages of greater rapidity and ease of performance.

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Bioluminescence screening for bacteriuria

Cited by 19 publications

References 13 publications

Reliability of a bioluminescence ATP assay for detection of bacteria

Reliability of a bioluminescence ATP assay for detection of bacteria

An Intact Cell Bioluminescence-Based Assay for the Simple and Rapid Diagnosis of Urinary Tract Infection

Simplified Technique for Detection of Significant Bacteriuria by Microscopic Examination of Urine

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