Bioluminescence Imaging In Vivo Confirms the Viability of Pancreatic Islets Transplanted into the Greater Omentum

Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats

“…Transplanting pancreatic islets into the omentum was shown to be a promising alternative method that was as effective as the conventional method of transplanting pancreatic islets into the liver [ 23 ]. The omentum has a large surface area that is easily accessible for surgery.…”

“…Isolated pancreatic islets in ECM skeletons were transplanted into the greater omentum ( n = 4) as described in our previous study [ 23 ]. Briefly, after a laparotomy the greater omentum was pulled out of the abdominal cavity and spread out on a sterile field.…”

“…This approach could provide a rich vascular supply but would avoid direct contact with the blood [ 21 ]. Experimental data [ 2 , 22 , 23 ] and first clinical experiences [ 24 , 25 ] have suggested that this approach has potential, but to date, it has not replaced hepatic placement. Therefore, we reasoned that pancreatic ECM skeletons, with accurate microstructure and biochemical composition, might provide an optimal tissue-specific environment for isolated islets.…”

Poly(4-Styrenesulfonic Acid-co-maleic Anhydride)-Coated NaGdF4:Yb,Tb,Nd Nanoparticles with Luminescence and Magnetic Properties for Imaging of Pancreatic Islets and β-Cells

“…To reverse diabetes, the islet cell transplantation has emerged as a promising alternative to traditional insulin therapy, which is potentially equally effective but much safer than whole pancreas transplantation, reducing severe hypoglycemic episodes and hemoglobin levels. However, in diabetes diagnostics, it is difficult to quantify the mass of β-cells or at least provide a suitable in vivo nanoparticle-based monitoring of the transplantation in early stages to detect islet graft rejection. , Hence, different imaging modalities for in vivo pancreatic β-cell and islet visualization have been suggested, ,, including MRI, although no method is so far routinely applicable in clinical practice. To visualize pancreatic islets and β-cells, not only magnetic iron oxide nanoparticles but also other types of materials including lanthanide-based, manganese and zinc, silver, fluorine, and gold particles have been developed. − Their major drawbacks consist in the toxicity and low in vivo sensitivity and specificity due to the absence of correlation between the particle concentration and 1 H NMR signal .…”

“…However, in diabetes diagnostics, it is difficult to quantify the mass of β-cells 14 or at least provide a suitable in vivo nanoparticle-based monitoring of the transplantation in early stages to detect islet graft rejection. 12,15 Hence, different imaging modalities for in vivo pancreatic β-cell and islet visualization have been suggested, 12,16,17 including MRI, 18 although no method is so far routinely applicable in clinical practice. To visualize pancreatic islets and β-cells, not only magnetic iron oxide nanoparticles 19 but also other types of materials including lanthanide-based, 20 manganese and zinc, 21 silver, 22 fluorine, 23 and gold particles have been developed.…”