Bioluminescence assay for cell viability

Lomakina, G. Yu.; Modestova, Yulia; Ugarova, N. N.

doi:10.1134/s0006297915060061

Cited by 75 publications

(55 citation statements)

References 62 publications

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“…ATP decrement is usually linked to a cytotoxic effect; nevertheless, Wickersham et al observed that metabolic stressed keratinocytes were more active in the response to bacterial infections. This finding is particularly attractive for our research, in fact, HaCaT knockout for NCSTN after irradiation could be more prone in fighting against infections, an issue that in HS lesions occurs. …”

Section: Resultsmentioning

confidence: 99%

“…[36] ATP decrement is usually linked to a cytotoxic effect; nevertheless, Wickersham et al observed that metabolic stressed keratinocytes were more active in the response to bacterial infections. [37,38] This finding is particularly attractive for our research, in fact, HaCaT knockout for NCSTN after irradiation could be more prone in fighting against infections, an issue that in HS lesions occurs. [39,40] 3.2 | PBM stimulated wound closure and keratinocytes migration without affecting proliferation or influencing cell cycle progression Skin keratinocytes are key cellular component of the epidermis that, after injuries such as wounds, are essential during the process of reepithelialization.…”

Section: Pbm-induced Metabolic Activation Of Hacat Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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Mutations in NCSTN gene (encoding for nicastrin protein) are associated with hidradenitis suppurativa (HS), a chronic inflammatory disease involving hair follicles. HS is clinically handled with drugs but the most severe cases are treated with surgery. Photobiomodulation (PBM) therapy, already used in the treatment of skin diseases such as acne, herpes virus lesions, ultraviolet damage, vitiligo, hypertrophic scar, keloid, burn, psoriasis and diabetic chronic wounds, could be beneficial as an adjuvant supportive treatment to promote and foster the healing process after skin excision in HS. The effects of PBM therapy in promoting the wound closure are evaluated in a HaCaT cells NCSTN-/-, assessing cell metabolism, migration rate, proliferation and cell cycle progression. In our experimental model, PBM exerts a potent action on metabolism of mutated keratinocytes, incrementing adenosine triphosphate (ATP) production at 2 hours, while after 24 hours an increase of metabolism with a decrement of intracellular ATP levels were recorded. Moreover, PBM speeds up the wound closure, inducing cells' migration without affecting their proliferation.Based on our findings, we suggest the use of PBM in HS patients, who undergo major surgery with large skin excision.

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Section: Resultsmentioning

confidence: 99%

Section: Pbm-induced Metabolic Activation Of Hacat Cellsmentioning

confidence: 99%

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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“…The ATP concentration can be quickly and easily determined using bioluminescence with great accuracy, even with an ultralow content of the substance in the sample (as low as 10 −14 ÷ 10 −12 M), using luciferin-luciferase reagents [16,17]. Portable luminometers make measuring the ATP concentration possible both in the laboratory and in situ, providing a method for quickly analyzing the presence of cells in test samples and their energy status.…”

Section: Introductionmentioning

confidence: 99%

A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

et al. 2019

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The possible use of the concentration of intracellular adenosine triphosphate (ATP) as a parameter enabling quick and adequate evaluation of the metabolic activity of methanogenic cells was demonstrated in the work. This approach was used to analyze the effect of introducing potassium humate and fulvic acids (1-10 g/L) into media with four different methanogenic consortia producing biogas. The ATP concentration was analyzed by the bioluminescent luciferin-luciferase method at the beginning and end of the process. During the entire process, the biogas composition, biogas efficiency, and the kinetics of methanogenesis in the presence of humic compounds were determined. The increase in the concentration of potassium humate led to a decrease in the overall energy status of the cells and reduced methanogenesis efficiency. However, fulvic acids introduced into the media stimulated methanogenesis in half of the tested consortia, which was accompanied by an increase in ATP concentration in cell samples. So, a positive correlation between the metabolic activity of cells in biogas formation and the concentration of ATP was observed. ATP concentration control appears to be an attractive tool for finding compounds that suppress methanogenesis in landfills.

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“…Cell viability evaluation by the ATP assay is the fastest, most sensitive and least prone to artefacts than other method. Additionally, the luminescent signal stabilizes 10 min after the addition of the reagent and becomes half quenched after 5 h [54]. Variations into ATP assay sensitivity are much more due to pipetting errors than a result of the chemistry behind the assay.…”

Section: Atp Assaymentioning

confidence: 99%

Challenges and Opportunities in Preclinical Research of Synthetic Cannabinoids for Pain Therapy

et al. 2020

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Cannabis has been used in pain management since 2900 BC. In the 20th century, synthetic cannabinoids began to emerge, thus opening the way for improved efficacy. The search for new forms of synthetic cannabinoids continues and, as such, the aim of this review is to provide a comprehensive tool for the research and development of this promising class of drugs. Methods for the in vitro assessment of cytotoxic, mutagenic or developmental effects are presented, followed by the main in vivo pain models used in cannabis research and the results yielded by different types of administration (systemic versus intrathecal versus inhalation). Animal models designed for assessing side-effects and long-term uses are also discussed. In the second part of this review, pharmacokinetic and pharmacodynamic studies of synthetic cannabinoid biodistribution, together with liquid chromatography–mass spectrometric identification of synthetic cannabinoids in biological fluids from rodents to humans are presented. Last, but not least, different strategies for improving the solubility and physicochemical stability of synthetic cannabinoids and their potential impact on pain management are discussed. In conclusion, synthetic cannabinoids are one of the most promising classes of drugs in pain medicine, and preclinical research should focus on identifying new and improved alternatives for a better clinical and preclinical outcome.

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Bioluminescence assay for cell viability

Cited by 75 publications

References 62 publications

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

Challenges and Opportunities in Preclinical Research of Synthetic Cannabinoids for Pain Therapy

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