Bioluminescence Analysis of Antibacterial Photodynamic Therapy Using Methylene Blue Mediated by Low-Intensity Level Laser Against Cariogenic Biofilms

Florez, Fernando Luis Esteban; Oliveira, Marina Reis; Júnior, Osmir Batista de Oliveira; Hiers, Rochelle Denise; Khajotia, Sharukh S.; Pretel, Hermes

doi:10.1089/pho.2017.4326

Cited by 14 publications

(10 citation statements)

References 42 publications

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“…The systematic review showed that among the cariogenic microorganisms listed in the selected studies, the most studied microorganism was Streptococcus mutans (82%) [12,14,15,16,17,18,19,20,21,23,24,25,26,27,28,29,31,32,33,34,35,37,39,40,41,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…The success of PDT depends on factors such as the administrated dose of light in the target cells and the time of exposure to light [46]. Considering these factors, we found that the most widely used light source was the red LED (32%) with wavelengths ranging from 625 to 670 nm [12,15,16,17,18,21,24,29,30,32,36,40], the most commonly used PS was methylene blue [13,16,18,21,23,24,27,28,29,30,32,33,36,38,39,42], and the most widely used pre-irradiation time was 5 min [12,14,15,16,17,19,20,21,22,27,28,30,31,32,33,39,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the ability to reduce the number of viable bacteria, most articles showed less than three logs of reduction [13,17,20,21,22,25,27,28,29,31,32,35,37,38,40,42,44,45]. The most commonly used control group was a negative control with no intervention.…”

Section: Resultsmentioning

confidence: 99%

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Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

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The aim of this study was to perform a systematic review of the literature followed by a meta-analysis about the efficacy of photodynamic therapy (PDT) on the microorganisms responsible for dental caries. The research question and the keywords were constructed according to the PICO strategy. The article search was done in Embase, Lilacs, Scielo, Medline, Scopus, Cochrane Library, Web of Science, Science Direct, and Pubmed databases. Randomized clinical trials and in vitro studies were selected in the review. The study was conducted according the PRISMA guideline for systematic review. A total of 34 articles were included in the qualitative analysis and four articles were divided into two subgroups to perform the meta-analysis. Few studies have achieved an effective microbial reduction in microorganisms associated with the pathogenesis of dental caries. The results highlight that there is no consensus about the study protocols for PDT against cariogenic microorganisms, although the results showed the PDT could be a good alternative for the treatment of dental caries.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

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“…The photosensitizer SAPYR (2-((4-pyridinyl)methyl)-1H-phenalen-1-one chloride) destroyed bacteria, embedded in extracellular polymeric substance to an extent that is regarded as disinfection [38]. In addition, methylene blue in combination with a low-intensity laser was successfully used to control cariogenic-like biofilms formed by Streptococcus mutans [40].…”

Section: Introductionmentioning

confidence: 99%

What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

et al. 2019

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Due to the increasing development of antibiotic resistances in recent years, scientists search intensely for new methods to control bacteria. Photodynamic treatment with porphyrins such as chlorophyll derivatives is one of the most promising methods to handle bacterial infestation, but their use is dependent on illumination and they seem to be more effective against Gram-positive bacteria than against Gram-negatives. In this study, we tested chlorophyllin against three bacterial model strains, the Gram-positive Bacillus subtilis 168, the Gram-negative Escherichia coli DH5α and E. coli strain NR698 which has a deficient outer membrane, simulating a Gram-negative “without” its outer membrane. Illuminated with a standardized light intensity of 12 mW/cm2, B. subtilis showed high sensitivity already at low chlorophyllin concentrations (≤105 cfu/mL: ≤0.1 mg/L, 106–108 cfu/mL: 0.5 mg/L), whereas E. coli DH5α was less sensitive (≤105 cfu/mL: 2.5 mg/L, 106 cfu/mL: 5 mg/L, 107–108 cfu/mL: ineffective at ≤25 mg/L chlorophyllin). E. coli NR698 was almost as sensitive as B. subtilis against chlorophyllin, pointing out that the outer membrane plays a significant role in protection against photodynamic chlorophyllin impacts. Interestingly, E. coli NR698 and B. subtilis can also be inactivated by chlorophyllin in darkness, indicating a second, light-independent mode of action. Thus, chlorophyllin seems to be more than a photosensitizer, and a promising substance for the control of bacteria, which deserves further investigation.

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“…Studies have demonstrated that antimicrobial photodynamic therapy (aPDT), have also been developed for the treatment of dental caries. [22–27] The photodynamic action occurs when a photosensitizing agent absorbs photons from the light source and its electrons enter an excited singlet state. [28] Different classes of chemical compounds, such as phenothiazines, phthalocyanines, and porphyrins, which have photoactive properties, have been effective in aPDT.…”

Section: Introductionmentioning

confidence: 99%

The effect of antimicrobial photodynamic therapy on infected dentin in primary teeth

et al. 2019

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Background: Antimicrobial photodynamic therapy (aPDT) has been used for the treatment of dental caries. Papacarie is a gel composed of papain and chloramine employed for the partial removal of carious tissue, effective against bacteria, however, some studies report that this antibacterial action is not quite so evident. The aim of this study is to evaluate the clinical effect of aPDT on infected dentin in dental caries lesion in primary teeth. Methods: Thirty-two primary molars with deep occlusal dental caries will be selected and divided in 2 groups: G1 – caries removal with a low-speed drill and G2 – application of aPDT with PapacarieMBlue. After treatment, all the teeth will be restored with glass ionomer cement and followed up clinically and radiographically, with evaluations at 3, 6, and 12 months. Dentin samples before and after treatment will be analyzed microbiologically. The data will be submitted to descriptive statistical analysis of the association between the categorical variables and both age and gender using the chi-square test and Fisher exact text. The Student t test and analysis of variance will be used for the comparison of mean signs and symptoms of reversible pulpitis. Pearson correlation coefficients will be calculated for the analysis of correlations among the continuous variables. Discussion: Adding methylene blue dye to the formula of PapacarieMBlue might potentiate the antimicrobial action of aPDT and work more effectively on the infected dentin combined with a conservative, minimally invasive treatment. Trial registration: NCT02734420 on 10 march 2016.

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Bioluminescence Analysis of Antibacterial Photodynamic Therapy Using Methylene Blue Mediated by Low-Intensity Level Laser Against Cariogenic Biofilms

Abstract: This study demonstrated that aPDT has promising potential to be used as an additional method to control oral cariogenic biofilms.

Cited by 14 publications

References 42 publications

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

The effect of antimicrobial photodynamic therapy on infected dentin in primary teeth

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