Biology of aging: Oxidative stress and RNA oxidation

Hemagirri, Manisekaran; Sasidharan, Sreenivasan

doi:10.1007/s11033-022-07219-1

Cited by 12 publications

(6 citation statements)

References 155 publications

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“…Aging is a complex process that is influenced not only by genetic factors but also by factors such as the environment and lifestyle. Organisms’ ability to regulate internal and external damage gradually weakens during their life cycle, leading to a physiological reduction of various biological activities ( Hemagirri and Sasidharan, 2022 ). It is an inevitable and irreversible process of organism degeneration with age.…”

Section: Discussionmentioning

confidence: 99%

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans

Liu

et al. 2023

Front. Pharmacol.

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Curcumin is well known as a potent antioxidant and free radical scavenger and has great potential for anti-aging applications. In this study, we investigate the molecular mechanism of curcumin in prolonging the lifespan of C. elegans. Four concentrations of curcumin (10, 25, 50, and 100 µM) were administered, and the optimal treatment concentration was determined by analyzing the nematode lifespan, physiology, and biochemistry. Additionally, RNA-seq and qRT-PCR were performed to explore the antioxidant effect of curcumin and its underlying mechanism. Results revealed that curcumin could significantly improve the survival capacity of C. elegans without influencing its growth. Curcumin was observed to significantly decrease the levels of reactive oxygen species (ROS) under extreme conditions such as heat stress and paraquat stress. In addition, curcumin increased the amount of nematode mitochondrial DNA (mtDNA) replication. RNA-seq results revealed that the underlying mechanism of curcumin in C. elegans is related to the mitogen-activated protein kinase (MAPK) pathway. qRT-PCR results confirmed that the expression of oxidative stress-related genes (sod-1, sod-2, sod-3, gst-4) was increased, and the expression of MAPK signaling pathway-related genes (sek-1, pmk-1, nsy-1) was significantly downregulated. Furthermore, the administration of curcumin extended the lifespan of nematodes, potentially through the enhancement of oxidative stress resistance and the downregulation of the MAPK signaling pathway. These findings improve our understanding of both lifespan extension and the potential mechanism of curcumin in C. elegans.

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Section: Discussionmentioning

confidence: 99%

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans

Liu

et al. 2023

Front. Pharmacol.

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“…Among the techniques proposed to delay aging and related diseases is that of targeting ROS-producing senescent cells (52). According to Denham Harman's 1956 free radical theory of aging (FRTA), the loss of function that occurs in old age results from the cellular accumulation of free radicals (62). In addition to having confirmed this theory, more recent studies have highlighted that oxidative stress can also play a role in pathologies whose frequency increases with age, such as cancer, neurodegeneration, arthritis, atherosclerosis, and metabolic pathologies such as diabetes and obesity (63)(64)(65).…”

Section: Ros and Rns Role In Agingmentioning

confidence: 99%

The janus face of oxidative stress in health and disease: The cause or the cure?

Francati,

Fiore,

Ferraguti

2023

Biomedical Reviews

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Reactive oxygen species (ROS), reactive nitrogen species (RNS) and diffusible reactive species (DRS), under certain concentrations, play an important role at the physiological level. The role of antioxidant molecules, endogenous and exogenous, is to maintain adequate amounts of ROS and RNS. The increase in ROS and RNS due to an overproduction of these species or a decrease in antioxidant molecules leads to the phenomenon called oxidative stress. Oxidative stress is involved in the physiological process of aging but is implicated in pathologies such as some types of tumors, neurodegenerative and autoimmune disorders, male infertility, cardiovascular disorders such as atrial fibrillation, and lung disorders such as chronic obstructive pulmonary disorders and pulmonary hypertension. This involvement has aroused widespread interest, especially because many studies try to exploit it at a therapeutic level. The purpose of this review is to discuss pathologies in which oxidative stress has an important role and for this reason, it can be targeted for a therapeutic intervention to improve or cure the pathology; sometimes modulation of oxidative stress can be used to improve the effect of the therapy.

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“…Reactive oxygen species (ROS) and oxidative stress are crucial in both senescence phases since they modify the catalytic activity and conformation of proteins and alter protein-protein and protein-deoxyribonucleic acid (DNA) interactions and affect different specific signaling mechanisms. These events could favor cell cycle arrest and the senescent characteristic secretion of proinflammatory cytokines, chemokines, growth factors, matrix metalloproteinases, and insoluble protein components of the extracellular matrix [7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells

García-Trejo,

Gómez-Sierra,

Eugenio-Pérez

et al. 2024

Antioxidants

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D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to the enhanced production of reactive oxygen species (ROS), such as aging and senescence. This study aimed to evaluate the potential protective effect of curcumin on senescence and oxidative stress and endoplasmic reticulum stress induced by D-galactose treatment in Lilly Laboratories Culture-Porcine Kidney 1 (LLC-PK1) and human kidney 2 (HK-2) proximal tubule cell lines from pig and human, respectively. For senescence induction, cells were treated with 300 mM D-galactose for 120 h and, to evaluate the protective effect of the antioxidant, cells were treated with 5 µM curcumin for 24 h and subsequently treated with curcumin + D-galactose for 120 h. In LLC-PK1 cells, curcumin treatment decreased by 20% the number of cells positive for senescence-associated (SA)-β-D-galactosidase staining and by 25% the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and increased by 40% lamin B1 expression. In HK-2 cells, curcumin treatment increased by 60% the expression of proliferating cell nuclear antigen (PCNA, 50% Klotho levels, and 175% catalase activity. In both cell lines, this antioxidant decreased the production of ROS (20% decrease for LLC-PK1 and 10 to 20% for HK-2). These data suggest that curcumin treatment has a moderate protective effect on D-galactose-induced senescence in LLC-PK1 and HK-2 cells.

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Biology of aging: Oxidative stress and RNA oxidation

Cited by 12 publications

References 155 publications

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans

The janus face of oxidative stress in health and disease: The cause or the cure?

Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells

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