Biology and Physiology of <I>Colletotrichum acutatum</I> Strains Causing Strawberry’s Anthracnose

Abstract

Seven Colletotrichum acutatum strains isolated from strawberries were cultivated on various culture media and tested in vitro and in vivo on Fragaria x ananassa for anthracnose symptoms. PDA caused an optimum growth of all isolates, MEA, ML and Strawberry allowed a good but not optimal growth. Czapeck, Sabouraud and the organic medium from potato gave the lowest growth rate of all isolates. PDA allowed a good sporulation of isolates follow-up by strawberry, MEA; in contrast, ML, Czapeck and Sabouraud gave a low sporulation. The fungal development is maximal at 25˚C and 27˚C for all the isolates studied. No growth was observed at 5˚C and 37˚C. The studied strains developed at all pH values. They didn't develop at 0.6, 0.65 and 0.7 aw but mycelial growth was perfect at 1 and 0.95 aw. The in vitro test of the pathogenicity caused by C. acutatum strains on strawberry's leaves showed an increasing percentage of infection with time and different infection rates among C. acutatum strains, strain Ca6 having a pathogenic power very high compared to the other isolates. After in vivo inoculation and incubation of the seedlings, all isolates caused severe symptoms related to anthracnose on leaflets and petioles of the studied strawberry plants.

Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits

“…The test was performed in 3 replicates and repeated 3 times (n = 90). The symptoms and morphology of C. acutatum on strawberries were similar with previous studies (Kumvinit & Akarapisan, 2016;Es-Soufi et al, 2018).…”

Morphometric Analysis of Isolated Conidia of Various Species of Colletotrichum sp. from Avocado and Mango in Côte d'Ivoire

“…Similarly, Majumdar and Mandal 35 showed in their study that sporulation is a complex phenomenon controlled by the environment and genetic factors that may vary between isolates. These results differed from those of Leharwan et al 39 and Es-Soufi et al 40 revealed that sporulation of the C. gloeosporioides and C. acutatum was excellent in the PDA medium. Group 1 (KA31, KA14, KA15, KA06, KA20, KA40 and KA04) and group 2 (KA32, KA39, KA37, KA19, KA25, KA22, KA03, KA17 and KA26) are characterized by a dominance of spores with rounded ends (class 0) and a respective size between 14-16×4-5 µm and 15×5 µm.…”

Biocontrol Potential of <i>Bacillus amyloliquefaciens</i> Bc2 and <i>Trichoderma harzianum</i> TR against Strawberry Anthracnose under Laboratory and Field Conditions

“…Colletotrichum spp. require relatively warm temperatures and are mainly [28] [29], and we also found that the favorable temperature for optimal development of isolates of Colletotrichum acutatum is 25˚C ± 2˚C [30], and in this study the onset of symptoms of the disease begins in the month in February but only 2/500 plants were sick in this month. Gray mold can cause losses that can be severe, reaching 25% in the main crop and 37% in the second.…”