Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits

Khruengsai, Sarunpron; Pripdeevech, Patcharee; Tanapichatsakul, Chutima; Srisuwannapa, Chanin; DʼSouza, Priya; Panuwet, Parinya

doi:10.7717/peerj.11242

Cited by 19 publications

(8 citation statements)

References 42 publications

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“…According to the literature, VOCs previously detected for D. eschscholtzii GU11N include alcohols, chromones, and other aromatic compounds, dienes, ketones, aldehydes, and sesquiterpenes. 38,39,52 Among the volatile compounds that have shown statistical significance, we found α-cubebene (18), α-bisabolene, and guaizulene. These terpenes can cause damage to hyphal membranes and suppress conidial formation in A. niger.…”

Section: ■ Introductionmentioning

confidence: 83%

Differential Volatile Organic Compound Expression in the Interaction of Daldinia eschscholtzii and Mycena citricolor

Escudero-Leyva,

Quirós-Guerrero,

Vásquez-Chaves

et al. 2023

ACS Omega

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Fungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (also known as endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (Coffea arabica). Published results from research performed in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American leaf spot of coffee (Mycena citricolor). One of these isolates (GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii (Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor were investigated by analyzing its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspace solid-phase microextraction (HS-SPME) gas chromatography-mass spectrometry (GC-MS) analysis. Additionally, we used ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) to identify nonvolatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than those by the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M. citricolor through the release of VOCs containing a combination of 1,8-dimethoxynapththalene and terpene compounds affecting M. citricolor pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against M. citricolor pseudopilei; scanning electron microscopy (SEM) photographs confirmed structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds. However, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that D. eschscholtzii has potential as a biocontrol agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen’s reproductive structures.

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Section: ■ Introductionmentioning

confidence: 83%

Differential Volatile Organic Compound Expression in the Interaction of Daldinia eschscholtzii and Mycena citricolor

Escudero-Leyva,

Quirós-Guerrero,

Vásquez-Chaves

et al. 2023

ACS Omega

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“…Fungal endophytes from the genus Daldinia inhibit the growth of the plant pathogens Colletotrichum acutatum and Sclerotium rolfsii [ 78 , 79 ]. Daldinia eschscholtzii isolated from ginger, Zingiber officinale , and Stemona root, Stemona tuberosa , was found to produce 60 identifiable compounds, the major ones being elemicin (24%), benzaldehyde dimethyl acetal (8%), ethyl sorbate (7%), methyl geranate (6%), trans-sabinene hydrate (5%) and 3,5-dimethyl-4-heptanone (5%) [ 79 ]. Elemicin is reported as an effective antifungal against Colletotrichum gloeosporoides , C. nymphaeae and C. musae [ 79 ].…”

Section: Fungal Endophytes and Their Effects On Fungal Pathogensmentioning

confidence: 99%

“…Daldinia eschscholtzii isolated from ginger, Zingiber officinale , and Stemona root, Stemona tuberosa , was found to produce 60 identifiable compounds, the major ones being elemicin (24%), benzaldehyde dimethyl acetal (8%), ethyl sorbate (7%), methyl geranate (6%), trans-sabinene hydrate (5%) and 3,5-dimethyl-4-heptanone (5%) [ 79 ]. Elemicin is reported as an effective antifungal against Colletotrichum gloeosporoides , C. nymphaeae and C. musae [ 79 ]. Daldinia cf.…”

Section: Fungal Endophytes and Their Effects On Fungal Pathogensmentioning

confidence: 99%

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

Grabka

d’Entremont

Adams

et al. 2022

Plants

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Virtually all examined plant species harbour fungal endophytes which asymptomatically infect or colonize living plant tissues, including leaves, branches, stems and roots. Endophyte-host interactions are complex and span the mutualist–pathogen continuum. Notably, mutualist endophytes can confer increased fitness to their host plants compared with uncolonized plants, which has attracted interest in their potential application in integrated plant health management strategies. In this review, we report on the many benefits that fungal endophytes provide to agricultural plants against common non-insect pests such as fungi, bacteria, nematodes, viruses, and mites. We report endophytic modes of action against the aforementioned pests and describe why this broad group of fungi is vitally important to current and future agricultural practices. We also list an extensive number of plant-friendly endophytes and detail where they are most commonly found or applied in different studies. This review acts as a general resource for understanding endophytes as they relate to potential large-scale agricultural applications.

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“…This institute uses these PGPB to form a biofertilizer commercially available in the local market with the trade name of Faslon ka Jraseemi Teka (FKJT). The inhibition potential of PGPB strains against N. rubicola was evaluated using in-vitro dual culture techniques (Khruengsai et al, 2021). In this experiment, a solidified PDA Petri plate was an experimental replicate/unit.…”

Section: Isolation and Identification Of Neocosmospora Rubicolamentioning

confidence: 99%

A Bacterial Consortium and Synthetic Fertilizer Based Biocontrol Approach Against Potato Rot Disease “Neocosmospora rubicola”

et al. 2022

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Conventional management of stem rot disease of potato, caused by Neocosmospora rubicola, through fungicide application is an environmentally unfavorable practice that calls for an alternative biocontrol approach. Plant growth-promoting bacteria (PGPB) are known to not only promote plant growth but also control diseases caused by various fungi. The study was designed to evaluate the potential of three strains of PGPB and synthetic fertilizer to manage stem rot of potatoes. In the first experiment, PGPB strains Azotobacter chroococcum, Azospirillum lipoferum, and Pseudomonas putida and their combinations were evaluated on PDA medium against N. rubicola using the dual culture technique. All three bacterial strains were found effective in reducing the radial growth of the fungus maximum up to 91%. In the second experiment, in the presence of half and full recommended doses of fertilizer nitrogen (N) and phosphorus (P), the potato growing medium was inoculated with N. rubicola alone, and with combinations of N. rubicola and PGPB strains (bacterial formulation; BF). N. rubicola increased stem and tuber rot, and decreased tuber weight by 11% compared to the control. On the other hand, sole inoculation with BF significantly increased tuber weight. In addition, a combined inoculation of N. rubicola and BF, or N. rubicola inoculation a week prior to BF inoculation did not affect tuber weight compared to control. However, inoculation of BF a week prior to N. rubicola, controlled rot symptoms and increased tuber weight by 32%. An increase in P application favored the PGPB strains in controlling rot in tubers. The interaction effect of fertilizer N with the inoculation combinations was non-significant; however, the main impact of N was to increase rot in tubers and decrease in potato stems. Hence a prerequisite application of PGPB formulation proved to be an effective tool against N. rubicola infestation in potatoes.

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Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits

Cited by 19 publications

References 42 publications

Differential Volatile Organic Compound Expression in the Interaction of Daldinia eschscholtzii and Mycena citricolor

Differential Volatile Organic Compound Expression in the Interaction of Daldinia eschscholtzii and Mycena citricolor

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

A Bacterial Consortium and Synthetic Fertilizer Based Biocontrol Approach Against Potato Rot Disease “Neocosmospora rubicola”

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