Biologically Induced Hydrogen Production Drives High Rate/High Efficiency Microbial Electrosynthesis of Acetate from Carbon Dioxide

Jourdin, Ludovic; Lü, Yang; Flexer, Victoria; Keller, Jürg; Freguia, Stefano

doi:10.1002/celc.201500530

Cited by 129 publications

(103 citation statements)

References 71 publications

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“…The discovery of metals such as iron and nickel on the electrode surface could be concentrated enzymes, but microbial or pH induced precipitation of the metals as (hydr) oxides, sulfides, carbonates, phosphates, perhaps even as nanoparticles cannot be ruled out. The latter has been hypothesized by Jourdin et al who observed copper concentrated on the surface of a microbial electroacetogenic cathode 47 . Whether precipitated metals, extracellular enzymes, or cell envelope-bound components, it is clear that the redox active components and electrochemical activity are inseparable from activity by the biofilm community.…”

mentioning

confidence: 81%

Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Marshall¹,

Ross²,

Weisenhorn³

et al. 2016

Preprint

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Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. We assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. These molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.A microbial electrosynthesis system (MES) is a bioelectrochemical device that employs microbes to generate, or synthesize, valuable products from CO 2 and electrons from the cathode 1 . This technology has potential for the renewable generation of biofuels and commodity chemicals, therefore understanding which microbes and which metabolic pathways are involved on biocathodes is critical to improving the performance of MESs. Furthermore, this work has broader environmental implications including understanding ecological aspects of one carbon metabolism and extracellular electron transfer relevant to global biogeochemical cycling.Advances in microbial electrosynthesis and related biocathode-driven processes have primarily focused on the production of three compounds: hydrogen 2 , methane 3 , and acetate 4 . However, microbial electrosynthesis can be used to generate value-added multi-carbon compounds such as alcohols and various short-chain fatty acids 5 . To manipulate (and optimize) these systems it is valuable to understand the metabolic pathways and extracellular electron transfer (EET) enzymes or molecules involved in cathode oxidation, and we must determine how these cathodic electron transport components are coupled to energy conservation by the microbial cell. While an increasingly robust body of literature has been established relating to microbial communities metabolizing with an anodic electron acceptor 6-8 , much is still unclear about the diverse metabolic capabilities of microorganisms and communities growing on a cathode 9 . Recently, a multi-omics evaluation of a mixed-species, carbon

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confidence: 81%

Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Marshall¹,

Ross²,

Weisenhorn³

et al. 2016

Preprint

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“…These images are consistent with other investigations that found inorganic and organic materials deposited on the electrode surface after being exposed to conditions meant to inhibit or remove biofilms (Jourdin et al 2016b;Yates, Siegert & Logan 2014). …”

Section: Inorganic and Organic Microbial Surface Modification Involvesupporting

confidence: 92%

“…These findings support previous research which hypothesises that an electroactive biofilm can activate its electrode surface, enhancing the catalysis of HER even after the biomass has been killed (Yates, Siegert & Logan 2014;Jourdin et al 2016b). …”

Section: Cathodic Biofilm Activates Electrode Surface and Achieves Efsupporting

confidence: 91%

“…Firstly, the pH was decreased to 3 for 4 hours and the electrodes were removed and air-dried for a further 3 hours. Once the exposure to low pH and the air-drying process were completed, the electrodes were placed again in the reactor, and subsequently the BES and the recirculation tank were autoclaved and new tubes were used to prevent contamination following the procedure used by Jourdin et al 2016b. A new chronoamperometry test coupled with the TOGA sensor was done at the same cathode potential (-1.1 V) for 3 hours.…”

Section: Effect Of the Biofilm On Electron Fluxesmentioning

confidence: 99%

“…There was an increase in Na, P, Cl, O and K in the sterilised cathode compared to bare electrode controls. Those elements are believed not to play a role in HER (Jourdin et al 2016b). The organic content of the surface, based on protein quantification, was also assessed.…”

Section: Inorganic and Organic Microbial Surface Modification Involvementioning

confidence: 99%

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Bio-Electrochemical process for Metal and Sulfur Recovery from Acid Mine Drainage

Zamora¹,

Alonso²

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Water recycling and resource recovery from acid mine drainage (AMD) are increasingly being regarded as desirable practices with direct benefits for the environment and the operational and economic viability of the resources sector.This thesis proves the concept of a novel bioelectrochemical system (BES) for the direct electrode-driven resource recovery and practically permanent AMD treatment. The technology consists of a two-cell bioelectrochemical setup to enable the removal of sulfate from the ongoing reduction-oxidation sulfur cycle, thereby also reducing salinity, without external addition of chemicals.In particular, the goals of this thesis are: (i) to enrich a sulfate reducing bacterial community capable of directly utilising a carbon based cathode as electron donor for autotrophic sulfate reduction, or via bioelectrochemically-produced H 2 ; (ii) to elucidate the electron flux pathways of autotrophic sulfate reduction and microbial interactions in cathodic mixed cultures; (iii) to develop a high-rate sulfate reducing bioelectrochemical reactor based on high surface area electrode materials; (iv) to design and implement a combined chemical-free bioelectrochemical process that enables the sulfur, metal and water recovery from AMD.In order to elucidate whether cathodes can effectively release electrons and act as the only electron donor to support sulfate reduction process, the effect of cathode potential and inoculum source were evaluated using electrochemical tools, including the recording of chronoamperometry and cyclic/linear sweep voltammetry (Chapter 5). Electrochemical and off-gas analysis coupled to liquid phase sampling was carried out to determine the electron fluxes from the electrode to the final electron acceptor (sulfate) during autotrophic sulfate reduction (Chapter 6). Fluorescence in situ hybridization (FISH) and digital image analysis (DAIME) of the microbial communities in z-stack confirmed the microbial stratification. After obtaining a well-functioning biocathode for autotrophic sulfate reduction, its performance was experimentally optimised in terms of high-surface area electrode materials, like multi-wall carbon nanotubes on reticulated vitreous carbon (MWCNT-RVC) and carbon granules (CG) (Chapter 7). Finally, a novel BES was tested for AMD treatment , outcompeting methanogens and homoacetogens for the hydrogen without the need to add chemical inhibitors.The findings of this thesis show that inexpensive CG can achieve higher current-tosulfide efficiencies at lower power consumption than the nano-modified three-dimensional MWCNT-RVC. Sequencing analysis of the 16S rRNA gene on day 58 revealed that MWCNT-RVC retained the bacteria and archaea population from the inoculum, while CG electrode surface was able to select for bacteria over archaea.The feasibility to integrate a two-stage BES was proven with a lab-scale setup for AMD treatment. Using AMD, the BES operation enhanced the sulfate reduction rate ( The solid metal-sludge was 2 times less voluminous and 9 times more readily s...

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Principle and Product Overview of Bioelectrosynthesis

Zhang

Wei

2020

Bioelectrosynthesis

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Biologically Induced Hydrogen Production Drives High Rate/High Efficiency Microbial Electrosynthesis of Acetate from Carbon Dioxide

Cited by 129 publications

References 71 publications

Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Bio-Electrochemical process for Metal and Sulfur Recovery from Acid Mine Drainage

Principle and Product Overview of Bioelectrosynthesis

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